Tags

Type your tag names separated by a space and hit enter

Variation in consumption of human milk oligosaccharides by infant gut-associated strains of Bifidobacterium breve.
Appl Environ Microbiol. 2013 Oct; 79(19):6040-9.AE

Abstract

Human milk contains a high concentration of complex oligosaccharides that influence the composition of the intestinal microbiota in breast-fed infants. Previous studies have indicated that select species such as Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum can utilize human milk oligosaccharides (HMO) in vitro as the sole carbon source, while the relatively few B. longum subsp. longum and Bifidobacterium breve isolates tested appear less adapted to these substrates. Considering the high frequency at which B. breve is isolated from breast-fed infant feces, we postulated that some B. breve strains can more vigorously consume HMO and thus are enriched in the breast-fed infant gastrointestinal tract. To examine this, a number of B. breve isolates from breast-fed infant feces were characterized for the presence of different glycosyl hydrolases that participate in HMO utilization, as well as by their ability to grow on HMO or specific HMO species such as lacto-N-tetraose (LNT) and fucosyllactose. All B. breve strains showed high levels of growth on LNT and lacto-N-neotetraose (LNnT), and, in general, growth on total HMO was moderate for most of the strains, with several strain differences. Growth and consumption of fucosylated HMO were strain dependent, mostly in isolates possessing a glycosyl hydrolase family 29 α-fucosidase. Glycoprofiling of the spent supernatant after HMO fermentation by select strains revealed that all B. breve strains can utilize sialylated HMO to a certain extent, especially sialyl-lacto-N-tetraose. Interestingly, this specific oligosaccharide was depleted before neutral LNT by strain SC95. In aggregate, this work indicates that the HMO consumption phenotype in B. breve is variable; however, some strains display specific adaptations to these substrates, enabling more vigorous consumption of fucosylated and sialylated HMO. These results provide a rationale for the predominance of this species in breast-fed infant feces and contribute to a more accurate picture of the ecology of the developing infant intestinal microbiota.

Authors+Show Affiliations

Departments of Viticulture and Enology.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23892749

Citation

Ruiz-Moyano, Santiago, et al. "Variation in Consumption of Human Milk Oligosaccharides By Infant Gut-associated Strains of Bifidobacterium Breve." Applied and Environmental Microbiology, vol. 79, no. 19, 2013, pp. 6040-9.
Ruiz-Moyano S, Totten SM, Garrido DA, et al. Variation in consumption of human milk oligosaccharides by infant gut-associated strains of Bifidobacterium breve. Appl Environ Microbiol. 2013;79(19):6040-9.
Ruiz-Moyano, S., Totten, S. M., Garrido, D. A., Smilowitz, J. T., German, J. B., Lebrilla, C. B., & Mills, D. A. (2013). Variation in consumption of human milk oligosaccharides by infant gut-associated strains of Bifidobacterium breve. Applied and Environmental Microbiology, 79(19), 6040-9. https://doi.org/10.1128/AEM.01843-13
Ruiz-Moyano S, et al. Variation in Consumption of Human Milk Oligosaccharides By Infant Gut-associated Strains of Bifidobacterium Breve. Appl Environ Microbiol. 2013;79(19):6040-9. PubMed PMID: 23892749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variation in consumption of human milk oligosaccharides by infant gut-associated strains of Bifidobacterium breve. AU - Ruiz-Moyano,Santiago, AU - Totten,Sarah M, AU - Garrido,Daniel A, AU - Smilowitz,Jennifer T, AU - German,J Bruce, AU - Lebrilla,Carlito B, AU - Mills,David A, Y1 - 2013/07/26/ PY - 2013/7/30/entrez PY - 2013/7/31/pubmed PY - 2014/3/8/medline SP - 6040 EP - 9 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 79 IS - 19 N2 - Human milk contains a high concentration of complex oligosaccharides that influence the composition of the intestinal microbiota in breast-fed infants. Previous studies have indicated that select species such as Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum can utilize human milk oligosaccharides (HMO) in vitro as the sole carbon source, while the relatively few B. longum subsp. longum and Bifidobacterium breve isolates tested appear less adapted to these substrates. Considering the high frequency at which B. breve is isolated from breast-fed infant feces, we postulated that some B. breve strains can more vigorously consume HMO and thus are enriched in the breast-fed infant gastrointestinal tract. To examine this, a number of B. breve isolates from breast-fed infant feces were characterized for the presence of different glycosyl hydrolases that participate in HMO utilization, as well as by their ability to grow on HMO or specific HMO species such as lacto-N-tetraose (LNT) and fucosyllactose. All B. breve strains showed high levels of growth on LNT and lacto-N-neotetraose (LNnT), and, in general, growth on total HMO was moderate for most of the strains, with several strain differences. Growth and consumption of fucosylated HMO were strain dependent, mostly in isolates possessing a glycosyl hydrolase family 29 α-fucosidase. Glycoprofiling of the spent supernatant after HMO fermentation by select strains revealed that all B. breve strains can utilize sialylated HMO to a certain extent, especially sialyl-lacto-N-tetraose. Interestingly, this specific oligosaccharide was depleted before neutral LNT by strain SC95. In aggregate, this work indicates that the HMO consumption phenotype in B. breve is variable; however, some strains display specific adaptations to these substrates, enabling more vigorous consumption of fucosylated and sialylated HMO. These results provide a rationale for the predominance of this species in breast-fed infant feces and contribute to a more accurate picture of the ecology of the developing infant intestinal microbiota. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/23892749/Variation_in_consumption_of_human_milk_oligosaccharides_by_infant_gut_associated_strains_of_Bifidobacterium_breve_ L2 - https://journals.asm.org/doi/10.1128/AEM.01843-13?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -