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A chronocoulometric aptasensor based on gold nanoparticles as a signal amplification strategy for detection of thrombin.
Anal Biochem. 2013 Oct 15; 441(2):95-100.AB

Abstract

A sensitive chronocoulometric aptasensor for the detection of thrombin has been developed based on gold nanoparticle amplification. The functional gold nanoparticles, loaded with link DNA (LDNA) and report DNA (RDNA), were immobilized on an electrode by thrombin aptamers performing as a recognition element and capture probe. LDNA was complementary to the thrombin aptamers and RDNA was noncomplementary, but could combine with [Ru(NH₃)₆]³⁺ (RuHex) cations. Electrochemical signals obtained by RuHex that bound quantitatively to the negatively charged phosphate backbone of DNA via electrostatic interactions were measured by chronocoulometry. In the presence of thrombin, the combination of thrombin and thrombin aptamers and the release of the functional gold nanoparticles could induce a significant decrease in chronocoulometric signal. The incorporation of gold nanoparticles in the chronocoulometric aptasensor significantly enhanced the sensitivity. The performance of the aptasensor was further increased by the optimization of the surface density of aptamers. Under optimum conditions, the chronocoulometric aptasensor exhibited a wide linear response range of 0.1-18.5 nM with a detection limit of 30 pM. The results demonstrated that this nanoparticle-based amplification strategy offers a simple and effective approach to detect thrombin.

Authors+Show Affiliations

Key Laboratory of Eco-environments in the Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23896460

Citation

Jiao, Xiao Xia, et al. "A Chronocoulometric Aptasensor Based On Gold Nanoparticles as a Signal Amplification Strategy for Detection of Thrombin." Analytical Biochemistry, vol. 441, no. 2, 2013, pp. 95-100.
Jiao XX, Chen JR, Zhang XY, et al. A chronocoulometric aptasensor based on gold nanoparticles as a signal amplification strategy for detection of thrombin. Anal Biochem. 2013;441(2):95-100.
Jiao, X. X., Chen, J. R., Zhang, X. Y., Luo, H. Q., & Li, N. B. (2013). A chronocoulometric aptasensor based on gold nanoparticles as a signal amplification strategy for detection of thrombin. Analytical Biochemistry, 441(2), 95-100. https://doi.org/10.1016/j.ab.2013.07.023
Jiao XX, et al. A Chronocoulometric Aptasensor Based On Gold Nanoparticles as a Signal Amplification Strategy for Detection of Thrombin. Anal Biochem. 2013 Oct 15;441(2):95-100. PubMed PMID: 23896460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A chronocoulometric aptasensor based on gold nanoparticles as a signal amplification strategy for detection of thrombin. AU - Jiao,Xiao Xia, AU - Chen,Jing Rong, AU - Zhang,Xi Yuan, AU - Luo,Hong Qun, AU - Li,Nian Bing, Y1 - 2013/07/26/ PY - 2013/05/11/received PY - 2013/07/08/revised PY - 2013/07/17/accepted PY - 2013/7/31/entrez PY - 2013/7/31/pubmed PY - 2014/3/22/medline KW - Aptasensor KW - Chronocoulometry KW - Gold nanoparticles KW - Thrombin SP - 95 EP - 100 JF - Analytical biochemistry JO - Anal Biochem VL - 441 IS - 2 N2 - A sensitive chronocoulometric aptasensor for the detection of thrombin has been developed based on gold nanoparticle amplification. The functional gold nanoparticles, loaded with link DNA (LDNA) and report DNA (RDNA), were immobilized on an electrode by thrombin aptamers performing as a recognition element and capture probe. LDNA was complementary to the thrombin aptamers and RDNA was noncomplementary, but could combine with [Ru(NH₃)₆]³⁺ (RuHex) cations. Electrochemical signals obtained by RuHex that bound quantitatively to the negatively charged phosphate backbone of DNA via electrostatic interactions were measured by chronocoulometry. In the presence of thrombin, the combination of thrombin and thrombin aptamers and the release of the functional gold nanoparticles could induce a significant decrease in chronocoulometric signal. The incorporation of gold nanoparticles in the chronocoulometric aptasensor significantly enhanced the sensitivity. The performance of the aptasensor was further increased by the optimization of the surface density of aptamers. Under optimum conditions, the chronocoulometric aptasensor exhibited a wide linear response range of 0.1-18.5 nM with a detection limit of 30 pM. The results demonstrated that this nanoparticle-based amplification strategy offers a simple and effective approach to detect thrombin. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/23896460/A_chronocoulometric_aptasensor_based_on_gold_nanoparticles_as_a_signal_amplification_strategy_for_detection_of_thrombin_ DB - PRIME DP - Unbound Medicine ER -