TY - JOUR T1 - Extraction, chemical characterization and biological activity determination of broccoli health promoting compounds. AU - Ares,Ana M, AU - Nozal,María J, AU - Bernal,José, Y1 - 2013/07/16/ PY - 2013/04/30/received PY - 2013/06/25/revised PY - 2013/07/11/accepted PY - 2013/8/1/entrez PY - 2013/8/1/pubmed PY - 2014/3/13/medline KW - 1,2-benzenedithiol KW - 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) KW - 2-(N-morpholino)ethanesulfonic acid) KW - AA KW - AAS KW - ABTS KW - ACN KW - AES KW - Advanced characterization methods KW - AlCl(3) KW - Biological activity assessment KW - Broccoli KW - C(6)H(4)(SH)(2) KW - C(6)H(8)O(7) KW - Cl(3)CH KW - DAD KW - DCM KW - DHAA KW - DTT KW - ECD KW - EDTA KW - ESI KW - EtOAc KW - EtOH KW - Extraction techniques KW - FA KW - FAMEs KW - FCR KW - FES KW - FID KW - FLD KW - FMOC-Cl KW - FRAP KW - FTICR KW - FTIR KW - Fourier transform infrared spectroscopy KW - Fourier transform ion cyclotron resonance KW - GC×GC KW - GLSs KW - GRA KW - H(2)C(2)O(4) KW - H(2)O(2) KW - H(3)BO(3) KW - HAc KW - HCl KW - HClO(4) KW - HNO(3) KW - HSCCC KW - Health promoting compounds KW - IC KW - ICP KW - IEC KW - ITCs KW - KCl KW - KOH KW - LC KW - MAE KW - MECK KW - MES KW - MPA KW - MS/MS KW - MeOH KW - NAA KW - NADPH KW - NH(4)C(2)H(3)O(2) KW - NMR KW - NS KW - Na(2)B(4)O(7) KW - NaC(2)H(3)O(2) KW - NaF KW - NaH(2)PO(4) KW - NaNO(2) KW - NaOH KW - OES KW - ORAC KW - PBS KW - Phytochemicals KW - RPLC KW - RT-PCR KW - SDS KW - SEC KW - SF KW - SFE KW - SPE KW - TCA KW - TCD KW - TCEP KW - TEAC KW - THF KW - TLC KW - TOC KW - Tris–HCl KW - UPLC KW - USDA KW - United States Department of Agriculture KW - X-ray fluorescence KW - XRF KW - acetic acid KW - acetonitrile KW - aluminium chloride KW - ammonium acetate KW - ascorbic acid or vitamin C KW - atomic absorption spectroscopy KW - atomic emission spectroscopy KW - boric acid KW - chloroform KW - citric acid KW - clucosinolates KW - comprehensive two-dimensional gas chromatography KW - dehydroascorbic acid KW - dichloromethane or methylene chloride KW - diode array detector KW - dithiothreitol KW - electrochemical detector KW - electrospray ionization KW - ethanol KW - ethyl acetate KW - ethylenediaminetetraacetic acid KW - fatty acids methyl esters KW - ferric reducing antioxidant power KW - flame emission spectroscopy KW - flame ionization detector KW - fluorenylmethyloxycarbonyl chloride KW - fluorescence detector KW - folin-ciocalteu reagent KW - formic acid KW - glucoraphanin KW - high speed counter current chromatography KW - hydrochloric acid KW - hydrogen peroxide KW - inductively coupled plasma KW - ion chromatography KW - ion-exchange chromatography KW - isothiocyanates KW - liquid chromatographic KW - mercaptoethanol KW - meta-phosphoric acid KW - methanol KW - micellar electrokinetic chromatography KW - microwave assisted extraction KW - neutron activation analysis KW - nicotinamide adenine dinucleotide phosphate KW - nitric acid KW - not specified KW - nuclear magnetic resonance KW - optical emission spectroscopy KW - oxalic acid KW - oxygen radical absorbance capacity KW - perchloric acid KW - phosphate buffer saline KW - potassium chloride KW - potassium hydroxide KW - reverse phase liquid chromatography KW - reverse transcription polymerase chain reaction KW - size exclusion chromatography KW - sodium acetate KW - sodium borate KW - sodium dihydrogen phosphate KW - sodium dodecyl sulfate KW - sodium fluoride KW - sodium hydroxide KW - sodium nitrite KW - solid phase extraction KW - sulforaphane KW - supercritical fluid extraction KW - tandem mass spectrometry KW - tetrahydrofuran KW - thermal conductivity detector KW - thin layer chromatography KW - tocopherol KW - trichloroacetic acid KW - tris(2-carboxyethyl) phosphine KW - tris–hydrochloride KW - trolox equivalent antioxidant capacity KW - ultra performance liquid chromatography KW - β-MET SP - 78 EP - 95 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1313 N2 - Broccoli (Brassica oleracea L. var. Italica) contains substantial amount of health-promoting compounds such as vitamins, glucosinolates, phenolic compounds, and dietary essential minerals; thus, it benefits health beyond providing just basic nutrition, and consumption of broccoli has been increasing over the years. This review gives an overview on the extraction and separation techniques, as well as the biological activity of some of the above mentioned compounds which have been published in the period January 2008 to January 2013. The work has been distributed according to the different families of health promoting compounds discussing the extraction procedures and the analytical techniques employed for their characterization. Finally, information about the different biological activities of these compounds has been also provided. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/23899380/Extraction_chemical_characterization_and_biological_activity_determination_of_broccoli_health_promoting_compounds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(13)01095-9 DB - PRIME DP - Unbound Medicine ER -