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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in Tablets.
Indian J Pharm Sci. 2013 Jan; 75(1):31-5.IJ

Abstract

A simple, accurate, rapid and precise reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of cefpodoxime proxetil and dicloxacillin sodium in tablet. The chromatographic separation was carried out on kromasil C18 analytical column (250×4.6 mm; 5 μm) with a mixture of acetonitrile:methanol:trifloroacetic acid (0.001%) with pH 6.5 (30:50:20, v/v/v) as mobile phase; at a flow rate of 1.0 ml/min. UV detection was performed at 235 nm. The dicloxacillin sodium and cefpodoxime proxetil were eluted at 1.92 and 3.35 min, respectively. The peaks were eluted with better resolution. Calibration plots were linear over the concentration range 0.5-20 μg/ml for cefpodoxime proxetil (r(2)=0.9996) and 5-50 μg/ml for dicloxacillin sodium (r(2)=0.9987). The method was validated for accuracy, precision, linearity and specificity. The method was very sensitive with limit of detection 0.0726, 0.3685 μg/ml and limit of quantification 0.220, 1.116 μg/ml for cefpodoxime proxetil and dicloxacillin sodium, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of cefpodoxime proxetil and dicloxacillin sodium in bulk drug and tablet dosage form.

Authors+Show Affiliations

Center for Health Science Studies, Ganpat University, Ganpat Vidyanagar, Mehsana-384 011, India.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23901158

Citation

Acharya, D R., and Dipti B. Patel. "Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in Tablets." Indian Journal of Pharmaceutical Sciences, vol. 75, no. 1, 2013, pp. 31-5.
Acharya DR, Patel DB. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in Tablets. Indian J Pharm Sci. 2013;75(1):31-5.
Acharya, D. R., & Patel, D. B. (2013). Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in Tablets. Indian Journal of Pharmaceutical Sciences, 75(1), 31-5. https://doi.org/10.4103/0250-474X.113538
Acharya DR, Patel DB. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in Tablets. Indian J Pharm Sci. 2013;75(1):31-5. PubMed PMID: 23901158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in Tablets. AU - Acharya,D R, AU - Patel,Dipti B, PY - 2012/04/23/received PY - 2013/01/06/revised PY - 2013/01/10/accepted PY - 2013/8/1/entrez PY - 2013/8/1/pubmed PY - 2013/8/1/medline KW - Cefpodoxime proxetil KW - dicloxacillin sodium KW - reversed-phase high-performance liquid chromatographic method KW - tablet KW - validation SP - 31 EP - 5 JF - Indian journal of pharmaceutical sciences JO - Indian J Pharm Sci VL - 75 IS - 1 N2 - A simple, accurate, rapid and precise reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of cefpodoxime proxetil and dicloxacillin sodium in tablet. The chromatographic separation was carried out on kromasil C18 analytical column (250×4.6 mm; 5 μm) with a mixture of acetonitrile:methanol:trifloroacetic acid (0.001%) with pH 6.5 (30:50:20, v/v/v) as mobile phase; at a flow rate of 1.0 ml/min. UV detection was performed at 235 nm. The dicloxacillin sodium and cefpodoxime proxetil were eluted at 1.92 and 3.35 min, respectively. The peaks were eluted with better resolution. Calibration plots were linear over the concentration range 0.5-20 μg/ml for cefpodoxime proxetil (r(2)=0.9996) and 5-50 μg/ml for dicloxacillin sodium (r(2)=0.9987). The method was validated for accuracy, precision, linearity and specificity. The method was very sensitive with limit of detection 0.0726, 0.3685 μg/ml and limit of quantification 0.220, 1.116 μg/ml for cefpodoxime proxetil and dicloxacillin sodium, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of cefpodoxime proxetil and dicloxacillin sodium in bulk drug and tablet dosage form. SN - 0250-474X UR - https://www.unboundmedicine.com/medline/citation/23901158/Development_and_Validation_of_RP_HPLC_Method_for_Simultaneous_Estimation_of_Cefpodoxime_Proxetil_and_Dicloxacillin_Sodium_in_Tablets_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23901158/ DB - PRIME DP - Unbound Medicine ER -
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