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Behavior of non-O157 Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli strains on alfalfa sprouts.
J Food Prot. 2013 Aug; 76(8):1429-33.JF

Abstract

Data about the behavior of non-O157 Shiga toxin-producing Escherichia coli (non-O157 STEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), and enteropathogenic E. coli (EPEC) on seeds and alfalfa sprouts are not available. The behavior of STEC, EIEC, ETEC, and EPEC was determined during germination and sprouting of alfalfa seeds at 20 ± 2°C and 30 ± 2°C and on alfalfa sprouts at 3 ± 2°C. When alfalfa seeds were inoculated with STEC, EIEC, ETEC, or EPEC strains, all these diarrheagenic E. coli pathotypes (DEPs) grew during germination and sprouting of seeds, reaching counts of approximately 5 and 6 log CFU/g after 1 day at 20 ± 2°C and 30 ± 2°C, respectively. However, when the sprouts were inoculated after 1 day of seed germination and stored at 20 ± 2°C or 30 ± 2°C, no growth was observed for any DEP during sprouting at 20 ± 2°C or 30 ± 2°C for 9 days. Refrigeration reduced significantly (P < 0.0.5) the number of viable DEPs on sprouts after 20 days in storage; nevertheless, these decreases have no practical significance for the safety of the sprouts.

Authors+Show Affiliations

Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Centro Universitario, Carretera Pachuca-Tulancingo Km. 4.5, Mineral de la Reforma, Hidalgo, C.P. 42183, Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23905800

Citation

Gómez-Aldapa, Carlos A., et al. "Behavior of non-O157 Shiga Toxin-producing Escherichia Coli, Enteroinvasive E. Coli, Enteropathogenic E. Coli, and Enterotoxigenic E. Coli Strains On Alfalfa Sprouts." Journal of Food Protection, vol. 76, no. 8, 2013, pp. 1429-33.
Gómez-Aldapa CA, Rangel-Vargas E, Torres-Vitela Mdel R, et al. Behavior of non-O157 Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli strains on alfalfa sprouts. J Food Prot. 2013;76(8):1429-33.
Gómez-Aldapa, C. A., Rangel-Vargas, E., Torres-Vitela, M. d. e. l. . R., Villarruel-López, A., & Castro-Rosas, J. (2013). Behavior of non-O157 Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli strains on alfalfa sprouts. Journal of Food Protection, 76(8), 1429-33. https://doi.org/10.4315/0362-028X.JFP-13-060
Gómez-Aldapa CA, et al. Behavior of non-O157 Shiga Toxin-producing Escherichia Coli, Enteroinvasive E. Coli, Enteropathogenic E. Coli, and Enterotoxigenic E. Coli Strains On Alfalfa Sprouts. J Food Prot. 2013;76(8):1429-33. PubMed PMID: 23905800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Behavior of non-O157 Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli, and enterotoxigenic E. coli strains on alfalfa sprouts. AU - Gómez-Aldapa,Carlos A, AU - Rangel-Vargas,Esmeralda, AU - Torres-Vitela,M Del Refugio, AU - Villarruel-López,Angélica, AU - Castro-Rosas,Javier, PY - 2013/8/3/entrez PY - 2013/8/3/pubmed PY - 2014/1/1/medline SP - 1429 EP - 33 JF - Journal of food protection JO - J Food Prot VL - 76 IS - 8 N2 - Data about the behavior of non-O157 Shiga toxin-producing Escherichia coli (non-O157 STEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), and enteropathogenic E. coli (EPEC) on seeds and alfalfa sprouts are not available. The behavior of STEC, EIEC, ETEC, and EPEC was determined during germination and sprouting of alfalfa seeds at 20 ± 2°C and 30 ± 2°C and on alfalfa sprouts at 3 ± 2°C. When alfalfa seeds were inoculated with STEC, EIEC, ETEC, or EPEC strains, all these diarrheagenic E. coli pathotypes (DEPs) grew during germination and sprouting of seeds, reaching counts of approximately 5 and 6 log CFU/g after 1 day at 20 ± 2°C and 30 ± 2°C, respectively. However, when the sprouts were inoculated after 1 day of seed germination and stored at 20 ± 2°C or 30 ± 2°C, no growth was observed for any DEP during sprouting at 20 ± 2°C or 30 ± 2°C for 9 days. Refrigeration reduced significantly (P < 0.0.5) the number of viable DEPs on sprouts after 20 days in storage; nevertheless, these decreases have no practical significance for the safety of the sprouts. SN - 1944-9097 UR - https://www.unboundmedicine.com/medline/citation/23905800/Behavior_of_non_O157_Shiga_toxin_producing_Escherichia_coli_enteroinvasive_E__coli_enteropathogenic_E__coli_and_enterotoxigenic_E__coli_strains_on_alfalfa_sprouts_ DB - PRIME DP - Unbound Medicine ER -