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Attenuating activity of the ovary on LH response to GnRH during the follicular phase of the cycle.
Clin Endocrinol (Oxf). 2014 Mar; 80(3):439-43.CE

Abstract

OBJECTIVE

Oestradiol sensitizes the pituitary to GnRH, while gonadotrophin surge attenuating factor (GnSAF) may oppose this action. Using the LH response to GnRH during treatment with FSH as an in vivo bioassay for GnSAF, we tested the hypothesis that the augmented LH response to GnRH in the late follicular phase is related to reduced production of GnSAF from the ovulatory follicle.

DESIGN

Prospective intervention study.

PATIENTS

Ten healthy, normally cycling women.

MEASUREMENTS

The LH response to 10 μg GnRH i.v. (ΔLH) was investigated on days 2 and 3 and on days v (follicle size 16-17 mm) and v + 1 of cycle 1 (control) and cycle 2. On days 2 and v, a single s.c. injection of either normal saline (cycle 1) or 450 IU recombinant FSH (cycle 2) was given after the end of the GnRH experiment.

RESULTS

FSH injection increased both serum oestradiol and inhibin B. In cycle 1, ΔLH remained unchanged from days 2 to 3 but increased significantly from days v to v + 1. In contrast, in cycle 2, ΔLH decreased significantly from days 2 to 3 (P < 0·05) and showed a nonsignificant increase from day v to day v + 1. The percentage difference in ΔLH between cycle 1 and cycle 2 was similar on days 3 (-66·9 ± 17·5%) and v + 1 (-65·2 ± 3·6%).

CONCLUSIONS

These results suggest that during the follicular phase of the menstrual cycle, GnSAF is produced by small antral follicles, while the contribution of the ovulatory follicle is minimal.

Authors+Show Affiliations

Department of Obstetrics and Gynaecology, Faculty of Medicine, School of Health Sciences, University of Thessalia, Larissa, Greece; Department of Obstetrics and Gynaecology, Medical School, University of Thrace, Alexandroupolis, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

23909480

Citation

Dimitraki, Marina, et al. "Attenuating Activity of the Ovary On LH Response to GnRH During the Follicular Phase of the Cycle." Clinical Endocrinology, vol. 80, no. 3, 2014, pp. 439-43.
Dimitraki M, Messini CI, Dafopoulos K, et al. Attenuating activity of the ovary on LH response to GnRH during the follicular phase of the cycle. Clin Endocrinol (Oxf). 2014;80(3):439-43.
Dimitraki, M., Messini, C. I., Dafopoulos, K., Gioka, T., Koutlaki, N., Garas, A., Georgoulias, P., & Messinis, I. E. (2014). Attenuating activity of the ovary on LH response to GnRH during the follicular phase of the cycle. Clinical Endocrinology, 80(3), 439-43. https://doi.org/10.1111/cen.12306
Dimitraki M, et al. Attenuating Activity of the Ovary On LH Response to GnRH During the Follicular Phase of the Cycle. Clin Endocrinol (Oxf). 2014;80(3):439-43. PubMed PMID: 23909480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attenuating activity of the ovary on LH response to GnRH during the follicular phase of the cycle. AU - Dimitraki,Marina, AU - Messini,Christina I, AU - Dafopoulos,Konstantinos, AU - Gioka,Theodora, AU - Koutlaki,Nikoletta, AU - Garas,Antonios, AU - Georgoulias,Panagiotis, AU - Messinis,Ioannis E, Y1 - 2013/08/28/ PY - 2012/12/18/received PY - 2013/03/25/revised PY - 2013/06/12/revised PY - 2013/07/30/accepted PY - 2013/8/6/entrez PY - 2013/8/6/pubmed PY - 2014/10/2/medline SP - 439 EP - 43 JF - Clinical endocrinology JO - Clin Endocrinol (Oxf) VL - 80 IS - 3 N2 - OBJECTIVE: Oestradiol sensitizes the pituitary to GnRH, while gonadotrophin surge attenuating factor (GnSAF) may oppose this action. Using the LH response to GnRH during treatment with FSH as an in vivo bioassay for GnSAF, we tested the hypothesis that the augmented LH response to GnRH in the late follicular phase is related to reduced production of GnSAF from the ovulatory follicle. DESIGN: Prospective intervention study. PATIENTS: Ten healthy, normally cycling women. MEASUREMENTS: The LH response to 10 μg GnRH i.v. (ΔLH) was investigated on days 2 and 3 and on days v (follicle size 16-17 mm) and v + 1 of cycle 1 (control) and cycle 2. On days 2 and v, a single s.c. injection of either normal saline (cycle 1) or 450 IU recombinant FSH (cycle 2) was given after the end of the GnRH experiment. RESULTS: FSH injection increased both serum oestradiol and inhibin B. In cycle 1, ΔLH remained unchanged from days 2 to 3 but increased significantly from days v to v + 1. In contrast, in cycle 2, ΔLH decreased significantly from days 2 to 3 (P < 0·05) and showed a nonsignificant increase from day v to day v + 1. The percentage difference in ΔLH between cycle 1 and cycle 2 was similar on days 3 (-66·9 ± 17·5%) and v + 1 (-65·2 ± 3·6%). CONCLUSIONS: These results suggest that during the follicular phase of the menstrual cycle, GnSAF is produced by small antral follicles, while the contribution of the ovulatory follicle is minimal. SN - 1365-2265 UR - https://www.unboundmedicine.com/medline/citation/23909480/Attenuating_activity_of_the_ovary_on_LH_response_to_GnRH_during_the_follicular_phase_of_the_cycle_ L2 - https://doi.org/10.1111/cen.12306 DB - PRIME DP - Unbound Medicine ER -