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Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats.
Acta Physiol (Oxf). 2013 Oct; 209(2):156-66.AP

Abstract

AIMS

This study examined the interaction between reactive oxygen species and nitric oxide (NO) in mediating the decrease in renal blood flow (RBF) evoked by sympathetic renal nerve stimulation (RNS).

METHODS

Groups of male Wistar rats were subjected to RNS at different frequencies prior to, and following, an infusion of: (i) tempol, the superoxide dismutase (SOD) mimetic, (ii) tempol plus the hydrogen peroxide-degrading enzyme catalase (tem + cat), (iii) diethyldithiocarbamic acid (DETC), a SOD inhibitor, (iv) the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine methyl ester (L-NAME) alone, or (v) L-NAME followed by tempol, into the kidney cortico-medullary border (CMB). Blood perfusion within the cortical (CBP) and medullary (MBP) regions of the kidney was measured using Laser-Doppler flowmetry.

RESULTS

Infusion of tempol CMB significantly attenuated RNS-evoked reductions in CBP (by 22% at 8 Hz; P < 0.05), but not MBP. When tempol and catalase were co-infused to reduce both ROS and hydrogen peroxide (H2 O2), respectively, there was a significantly greater attenuation of the RNS-evoked reduction in CBP compared with that of tempol alone. Infusion of either DETC or L-NAME alone did not significantly affect the CBP or MBP responses to RNS. Similarly, RNS following tempol infusion with L-NAME also had no effect on CBP and MBP over and above the group that received tempol alone.

CONCLUSION

These results suggest that reactive oxygen species such as superoxide and H2 O2 have a direct role in reducing renal vascular compliance in response to RNS, rather than indirectly through scavenging NO.

Authors+Show Affiliations

Department of Physiology, University College Cork, Cork, Ireland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23910436

Citation

Ahmeda, A F., et al. "Effect of Reactive Oxygen Species and Nitric Oxide in the Neural Control of Intrarenal Haemodynamics in Anaesthetized Normotensive Rats." Acta Physiologica (Oxford, England), vol. 209, no. 2, 2013, pp. 156-66.
Ahmeda AF, Rae MG, Johns EJ. Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats. Acta Physiol (Oxf). 2013;209(2):156-66.
Ahmeda, A. F., Rae, M. G., & Johns, E. J. (2013). Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats. Acta Physiologica (Oxford, England), 209(2), 156-66. https://doi.org/10.1111/apha.12150
Ahmeda AF, Rae MG, Johns EJ. Effect of Reactive Oxygen Species and Nitric Oxide in the Neural Control of Intrarenal Haemodynamics in Anaesthetized Normotensive Rats. Acta Physiol (Oxf). 2013;209(2):156-66. PubMed PMID: 23910436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats. AU - Ahmeda,A F, AU - Rae,M G, AU - Johns,E J, Y1 - 2013/08/27/ PY - 2012/11/29/received PY - 2013/01/15/revised PY - 2013/07/15/revised PY - 2013/07/24/accepted PY - 2013/8/6/entrez PY - 2013/8/6/pubmed PY - 2014/4/12/medline KW - nitric oxide KW - reactive oxygen species KW - renal blood pressure KW - renal nerve stimulation SP - 156 EP - 66 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 209 IS - 2 N2 - AIMS: This study examined the interaction between reactive oxygen species and nitric oxide (NO) in mediating the decrease in renal blood flow (RBF) evoked by sympathetic renal nerve stimulation (RNS). METHODS: Groups of male Wistar rats were subjected to RNS at different frequencies prior to, and following, an infusion of: (i) tempol, the superoxide dismutase (SOD) mimetic, (ii) tempol plus the hydrogen peroxide-degrading enzyme catalase (tem + cat), (iii) diethyldithiocarbamic acid (DETC), a SOD inhibitor, (iv) the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine methyl ester (L-NAME) alone, or (v) L-NAME followed by tempol, into the kidney cortico-medullary border (CMB). Blood perfusion within the cortical (CBP) and medullary (MBP) regions of the kidney was measured using Laser-Doppler flowmetry. RESULTS: Infusion of tempol CMB significantly attenuated RNS-evoked reductions in CBP (by 22% at 8 Hz; P < 0.05), but not MBP. When tempol and catalase were co-infused to reduce both ROS and hydrogen peroxide (H2 O2), respectively, there was a significantly greater attenuation of the RNS-evoked reduction in CBP compared with that of tempol alone. Infusion of either DETC or L-NAME alone did not significantly affect the CBP or MBP responses to RNS. Similarly, RNS following tempol infusion with L-NAME also had no effect on CBP and MBP over and above the group that received tempol alone. CONCLUSION: These results suggest that reactive oxygen species such as superoxide and H2 O2 have a direct role in reducing renal vascular compliance in response to RNS, rather than indirectly through scavenging NO. SN - 1748-1716 UR - https://www.unboundmedicine.com/medline/citation/23910436/Effect_of_reactive_oxygen_species_and_nitric_oxide_in_the_neural_control_of_intrarenal_haemodynamics_in_anaesthetized_normotensive_rats_ L2 - https://doi.org/10.1111/apha.12150 DB - PRIME DP - Unbound Medicine ER -