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Mechanisms involved in abdominal nociception induced by either TRPV1 or TRPA1 stimulation of rat peritoneum.
Eur J Pharmacol. 2013 Aug 15; 714(1-3):332-44.EJ

Abstract

Abdominal pain is a frequent symptom of peritoneal cavity irritation, but little is known about the role of the receptors for irritant substances, transient receptor potential vanilloid 1 (TRPV1) and ankyrin 1 (TRPA1), in this painful condition. Thus, we investigated the abdominal nociception caused by peritoneal stimulation with TRPV1 (capsaicin) and TRPA1 (allyl isothiocyanate, AITC) agonists and their mechanisms in rats. The intraperitoneal (i.p.) injection of either capsaicin or AITC (0.03-10 mg/kg) induced short-term (up to 20 min) and dose-dependent abdominal nociception, and also produced c-fos expression in spinal afferents of the dorsal horn. TRPV1 antagonism prevented (94 ± 4% inhibition) nociception induced by capsaicin but not by AITC. In contrast, the TRPA1 antagonism almost abolished AITC-induced nociception (95 ± 2% inhibition) without altering the capsaicin response. Moreover, nociception induced by either capsaicin or AITC was reduced by the desensitisation of TRPV1-positive sensory fibres with resiniferatoxin (73 ± 18 and 76 ± 15% inhibitions, respectively) and by the NK1 receptor antagonist aprepitant (56 ± 5 and 53 ± 8% inhibitions, respectively). Likewise, the i.p. injections of capsaicin or AITC increased the content of substance P in the peritoneal fluid. Nevertheless, neither the mast cell membrane stabiliser cromoglycate, nor the H1 antagonist promethazine, nor depletion of peritoneal macrophages affected abdominal nociception induced either by capsaicin or AITC. Accordingly, neither capsaicin nor AITC increased the histamine content in the peritoneal fluid or provoked peritoneal mast cell degranulation in vitro. Collectively, our findings suggest that TRPV1 and TRPA1 stimulation in the peritoneum produces abdominal nociception that is mediated by sensory fibres activation.

Authors+Show Affiliations

Graduate Program in Biological Sciences, Toxicological Biochemistry, Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23911956

Citation

Trevisan, Gabriela, et al. "Mechanisms Involved in Abdominal Nociception Induced By Either TRPV1 or TRPA1 Stimulation of Rat Peritoneum." European Journal of Pharmacology, vol. 714, no. 1-3, 2013, pp. 332-44.
Trevisan G, Rossato MF, Hoffmeister C, et al. Mechanisms involved in abdominal nociception induced by either TRPV1 or TRPA1 stimulation of rat peritoneum. Eur J Pharmacol. 2013;714(1-3):332-44.
Trevisan, G., Rossato, M. F., Hoffmeister, C., Oliveira, S. M., Silva, C. R., Matheus, F. C., Mello, G. C., Antunes, E., Prediger, R. D., & Ferreira, J. (2013). Mechanisms involved in abdominal nociception induced by either TRPV1 or TRPA1 stimulation of rat peritoneum. European Journal of Pharmacology, 714(1-3), 332-44. https://doi.org/10.1016/j.ejphar.2013.07.029
Trevisan G, et al. Mechanisms Involved in Abdominal Nociception Induced By Either TRPV1 or TRPA1 Stimulation of Rat Peritoneum. Eur J Pharmacol. 2013 Aug 15;714(1-3):332-44. PubMed PMID: 23911956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms involved in abdominal nociception induced by either TRPV1 or TRPA1 stimulation of rat peritoneum. AU - Trevisan,Gabriela, AU - Rossato,Mateus F, AU - Hoffmeister,Carin, AU - Oliveira,Sara M, AU - Silva,Cássia R, AU - Matheus,Filipe C, AU - Mello,Gláucia C, AU - Antunes,Edson, AU - Prediger,Rui D S, AU - Ferreira,Juliano, Y1 - 2013/08/01/ PY - 2013/01/22/received PY - 2013/05/21/revised PY - 2013/07/12/accepted PY - 2013/8/6/entrez PY - 2013/8/6/pubmed PY - 2014/5/7/medline KW - Allyl isothiocyanate KW - Capsaicin KW - Mast cells KW - Pain KW - Resiniferatoxin KW - Substance p SP - 332 EP - 44 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 714 IS - 1-3 N2 - Abdominal pain is a frequent symptom of peritoneal cavity irritation, but little is known about the role of the receptors for irritant substances, transient receptor potential vanilloid 1 (TRPV1) and ankyrin 1 (TRPA1), in this painful condition. Thus, we investigated the abdominal nociception caused by peritoneal stimulation with TRPV1 (capsaicin) and TRPA1 (allyl isothiocyanate, AITC) agonists and their mechanisms in rats. The intraperitoneal (i.p.) injection of either capsaicin or AITC (0.03-10 mg/kg) induced short-term (up to 20 min) and dose-dependent abdominal nociception, and also produced c-fos expression in spinal afferents of the dorsal horn. TRPV1 antagonism prevented (94 ± 4% inhibition) nociception induced by capsaicin but not by AITC. In contrast, the TRPA1 antagonism almost abolished AITC-induced nociception (95 ± 2% inhibition) without altering the capsaicin response. Moreover, nociception induced by either capsaicin or AITC was reduced by the desensitisation of TRPV1-positive sensory fibres with resiniferatoxin (73 ± 18 and 76 ± 15% inhibitions, respectively) and by the NK1 receptor antagonist aprepitant (56 ± 5 and 53 ± 8% inhibitions, respectively). Likewise, the i.p. injections of capsaicin or AITC increased the content of substance P in the peritoneal fluid. Nevertheless, neither the mast cell membrane stabiliser cromoglycate, nor the H1 antagonist promethazine, nor depletion of peritoneal macrophages affected abdominal nociception induced either by capsaicin or AITC. Accordingly, neither capsaicin nor AITC increased the histamine content in the peritoneal fluid or provoked peritoneal mast cell degranulation in vitro. Collectively, our findings suggest that TRPV1 and TRPA1 stimulation in the peritoneum produces abdominal nociception that is mediated by sensory fibres activation. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/23911956/Mechanisms_involved_in_abdominal_nociception_induced_by_either_TRPV1_or_TRPA1_stimulation_of_rat_peritoneum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(13)00551-7 DB - PRIME DP - Unbound Medicine ER -