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Czech ethanol-free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens.
J Food Sci 2013; 78(9):M1421-9JF

Abstract

Propolis acts primarily as a biocide against invasive bacteria and fungi in the hive, suggesting its potential for industrial applications. In food application, propolis is considered as a chemical preservative in meat products, extending shelf life of frozen meat and other food. The mechanism of action is still unclear due to the synergy of multiple compounds contained in propolis and due to parallel targeting of multiple pathways within each affected organism. Here, we examined the antimicrobial properties of dimethylsulfoxide (DMSO) Czech propolis extract. Until recently, DMSO was only rarely used in the propolis studies, although the other solvents tested (mostly ethanol) may significantly affect the observed inhibitory effects, notwithstanding the antimicrobial effects of ethanol itself. Here, we provide results of zone inhibition tests against Aspergillus fumigatus, Microsporum gypseum, Microsporum canis, Candida albicans, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis. Although we determined inhibitory effects against all the microorganisms tested, the dose-dependent response curves were not similar to each other. While inhibitory effects against C. albicans or S. aureus were strictly dose-dependent, responses of M. gypseum and E. faecalis displayed plateau across the broad range of concentrations tested. Interestingly, response of E. coli revealed the double-peak dose-dependent curve, and responses of M. canis and L. monocytogenes decreased at the highest concentrations tested. Suggested is evaluation of DMSO propolis extracts in experimental treatment of human and veterinary infections, preferably in multitherapy with antibiotics.

Authors+Show Affiliations

Faculty of Science, Univ. of Hradec Králové, Rokitanského 62, CZ-500 03 Hradec Králové, Czech Republic.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23915150

Citation

Netíková, Ladislava, et al. "Czech Ethanol-free Propolis Extract Displays Inhibitory Activity Against a Broad Spectrum of Bacterial and Fungal Pathogens." Journal of Food Science, vol. 78, no. 9, 2013, pp. M1421-9.
Netíková L, Bogusch P, Heneberg P. Czech ethanol-free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. J Food Sci. 2013;78(9):M1421-9.
Netíková, L., Bogusch, P., & Heneberg, P. (2013). Czech ethanol-free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. Journal of Food Science, 78(9), pp. M1421-9. doi:10.1111/1750-3841.12230.
Netíková L, Bogusch P, Heneberg P. Czech Ethanol-free Propolis Extract Displays Inhibitory Activity Against a Broad Spectrum of Bacterial and Fungal Pathogens. J Food Sci. 2013;78(9):M1421-9. PubMed PMID: 23915150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Czech ethanol-free propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens. AU - Netíková,Ladislava, AU - Bogusch,Petr, AU - Heneberg,Petr, Y1 - 2013/08/05/ PY - 2013/02/19/received PY - 2013/06/24/accepted PY - 2013/8/7/entrez PY - 2013/8/7/pubmed PY - 2014/4/8/medline KW - Apidae KW - anti-infective agents KW - foodborne pathogens KW - microbicides KW - natural products SP - M1421 EP - 9 JF - Journal of food science JO - J. Food Sci. VL - 78 IS - 9 N2 - Propolis acts primarily as a biocide against invasive bacteria and fungi in the hive, suggesting its potential for industrial applications. In food application, propolis is considered as a chemical preservative in meat products, extending shelf life of frozen meat and other food. The mechanism of action is still unclear due to the synergy of multiple compounds contained in propolis and due to parallel targeting of multiple pathways within each affected organism. Here, we examined the antimicrobial properties of dimethylsulfoxide (DMSO) Czech propolis extract. Until recently, DMSO was only rarely used in the propolis studies, although the other solvents tested (mostly ethanol) may significantly affect the observed inhibitory effects, notwithstanding the antimicrobial effects of ethanol itself. Here, we provide results of zone inhibition tests against Aspergillus fumigatus, Microsporum gypseum, Microsporum canis, Candida albicans, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis. Although we determined inhibitory effects against all the microorganisms tested, the dose-dependent response curves were not similar to each other. While inhibitory effects against C. albicans or S. aureus were strictly dose-dependent, responses of M. gypseum and E. faecalis displayed plateau across the broad range of concentrations tested. Interestingly, response of E. coli revealed the double-peak dose-dependent curve, and responses of M. canis and L. monocytogenes decreased at the highest concentrations tested. Suggested is evaluation of DMSO propolis extracts in experimental treatment of human and veterinary infections, preferably in multitherapy with antibiotics. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/23915150/Czech_ethanol_free_propolis_extract_displays_inhibitory_activity_against_a_broad_spectrum_of_bacterial_and_fungal_pathogens_ L2 - https://doi.org/10.1111/1750-3841.12230 DB - PRIME DP - Unbound Medicine ER -