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Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia.
Neurosci Lett. 2013 Sep 27; 552:92-7.NL

Abstract

Both TRPV1 and TRPA1 are non-selective cation channels. They are co-expressed, and interact in sensory neurons such as dorsal root ganglia (DRG) and trigeminal ganglia (TG), and are involved in nociception, being activated by nociceptive stimuli. Immunohistological localization of TRPV1 in vestibular ganglion (VG) neurons has been reported. Although TRPA1 is co-expressed with TRPV1 in DRG and TG neurons, it is unclear whether TRPA1 channels are expressed in VG neurons. Moreover, it is unknown whether TRPV1 and TRPA1 channels are functional in VG neurons. We investigated the expression of TRPV1 and TRPA1 in rat VG neurons by RT-PCR, in situ hybridization, immunohistochemistry, and Ca(2+) imaging experiments. Both TRPV1 and TRPA1 RT-PCR products were amplified from the mRNA of rat VG neurons. In situ hybridization experiments showed TRPV1 and TRPA1 mRNA expression in the majority of VG neurons. Immunohistochemistry experiments confirmed TRPV1 protein expression. In Ca(2+) imaging experiments, capsaicin, a TRPV1 agonist, induced a significant increase in intracellular calcium ion concentration ([Ca(2+)]i) in rat primary cultured VG neurons, which was almost completely blocked by capsazepine, a TRPV1-specific antagonist. Cinnamaldehyde, a TRPA1 agonist, also caused an increase in [Ca(2+)]i, which was completely inhibited by HC030031, a TRPA1-specific antagonist. Moreover, in some VG neurons, a [Ca(2+)]i increase was evoked by both capsaicin and cinnamaldehyde in the same neuron. In summary, our histological and physiological studies reveal that TRPV1 and TRPA1 are expressed in VG neurons. It is suggested that TRPV1 and TRPA1 in VG neurons might participate in vestibular function and/or dysfunction such as vertigo.

Authors+Show Affiliations

Department of Otorhinolaryngology - Head and Neck Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; Department of Neuroscience and Cell Biology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23916509

Citation

Kamakura, Takefumi, et al. "Functional Expression of TRPV1 and TRPA1 in Rat Vestibular Ganglia." Neuroscience Letters, vol. 552, 2013, pp. 92-7.
Kamakura T, Ishida Y, Nakamura Y, et al. Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia. Neurosci Lett. 2013;552:92-7.
Kamakura, T., Ishida, Y., Nakamura, Y., Yamada, T., Kitahara, T., Takimoto, Y., Horii, A., Uno, A., Imai, T., Okazaki, S., Inohara, H., & Shimada, S. (2013). Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia. Neuroscience Letters, 552, 92-7. https://doi.org/10.1016/j.neulet.2013.07.019
Kamakura T, et al. Functional Expression of TRPV1 and TRPA1 in Rat Vestibular Ganglia. Neurosci Lett. 2013 Sep 27;552:92-7. PubMed PMID: 23916509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia. AU - Kamakura,Takefumi, AU - Ishida,Yusuke, AU - Nakamura,Yukiko, AU - Yamada,Takahiro, AU - Kitahara,Tadashi, AU - Takimoto,Yasumitsu, AU - Horii,Arata, AU - Uno,Atsuhiko, AU - Imai,Takao, AU - Okazaki,Suzuyo, AU - Inohara,Hidenori, AU - Shimada,Shoichi, Y1 - 2013/08/03/ PY - 2012/12/10/received PY - 2013/06/15/revised PY - 2013/07/15/accepted PY - 2013/8/7/entrez PY - 2013/8/7/pubmed PY - 2014/5/16/medline KW - Ca(2+) imaging experiment KW - In situ hybridization KW - TRPA1 KW - TRPV1 KW - Vestibular ganglia SP - 92 EP - 7 JF - Neuroscience letters JO - Neurosci Lett VL - 552 N2 - Both TRPV1 and TRPA1 are non-selective cation channels. They are co-expressed, and interact in sensory neurons such as dorsal root ganglia (DRG) and trigeminal ganglia (TG), and are involved in nociception, being activated by nociceptive stimuli. Immunohistological localization of TRPV1 in vestibular ganglion (VG) neurons has been reported. Although TRPA1 is co-expressed with TRPV1 in DRG and TG neurons, it is unclear whether TRPA1 channels are expressed in VG neurons. Moreover, it is unknown whether TRPV1 and TRPA1 channels are functional in VG neurons. We investigated the expression of TRPV1 and TRPA1 in rat VG neurons by RT-PCR, in situ hybridization, immunohistochemistry, and Ca(2+) imaging experiments. Both TRPV1 and TRPA1 RT-PCR products were amplified from the mRNA of rat VG neurons. In situ hybridization experiments showed TRPV1 and TRPA1 mRNA expression in the majority of VG neurons. Immunohistochemistry experiments confirmed TRPV1 protein expression. In Ca(2+) imaging experiments, capsaicin, a TRPV1 agonist, induced a significant increase in intracellular calcium ion concentration ([Ca(2+)]i) in rat primary cultured VG neurons, which was almost completely blocked by capsazepine, a TRPV1-specific antagonist. Cinnamaldehyde, a TRPA1 agonist, also caused an increase in [Ca(2+)]i, which was completely inhibited by HC030031, a TRPA1-specific antagonist. Moreover, in some VG neurons, a [Ca(2+)]i increase was evoked by both capsaicin and cinnamaldehyde in the same neuron. In summary, our histological and physiological studies reveal that TRPV1 and TRPA1 are expressed in VG neurons. It is suggested that TRPV1 and TRPA1 in VG neurons might participate in vestibular function and/or dysfunction such as vertigo. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/23916509/Functional_expression_of_TRPV1_and_TRPA1_in_rat_vestibular_ganglia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(13)00665-4 DB - PRIME DP - Unbound Medicine ER -