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Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication.
Antiviral Res. 2013 Oct; 100(1):98-101.AR

Abstract

Hepatitis E virus (HEV) is one of the most important causes of acute hepatitis worldwide. Although most infections are self-limiting, mortality is particularly high in pregnant women. Chronic infections can occur in transplant and other immune-compromised patients. Successful treatment of chronic hepatitis E has been reported with ribavirin and pegylated interferon-alpha, however severe side effects were observed. We employed the cutthroat trout virus (CTV), a non-pathogenic fish virus with remarkable similarities to HEV, as a potential surrogate for HEV and established an antiviral assay against this virus using the Chinook salmon embryo (CHSE-214) cell line. Ribavirin and the respective trout interferon were found to efficiently inhibit CTV replication. Other known broad-spectrum inhibitors of RNA virus replication such as the nucleoside analog 2'-C-methylcytidine resulted only in a moderate antiviral activity. In its natural fish host, CTV levels largely fluctuate during the reproductive cycle with the virus detected mainly during spawning. We wondered whether this aspect of CTV infection may serve as a surrogate model for the peculiar pathogenesis of HEV in pregnant women. To that end the effect of three sex steroids on in vitro CTV replication was evaluated. Whereas progesterone resulted in marked inhibition of virus replication, testosterone and 17β-estradiol stimulated viral growth. Our data thus indicate that CTV may serve as a surrogate model for HEV, both for antiviral experiments and studies on the replication biology of the Hepeviridae.

Authors+Show Affiliations

Rega Institute for Medical Research, Department of Microbiology and Immunology, Minderbroedersstraat 10, 3000 Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23916729

Citation

Debing, Yannick, et al. "Cutthroat Trout Virus as a Surrogate in Vitro Infection Model for Testing Inhibitors of Hepatitis E Virus Replication." Antiviral Research, vol. 100, no. 1, 2013, pp. 98-101.
Debing Y, Winton J, Neyts J, et al. Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication. Antiviral Res. 2013;100(1):98-101.
Debing, Y., Winton, J., Neyts, J., & Dallmeier, K. (2013). Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication. Antiviral Research, 100(1), 98-101. https://doi.org/10.1016/j.antiviral.2013.07.013
Debing Y, et al. Cutthroat Trout Virus as a Surrogate in Vitro Infection Model for Testing Inhibitors of Hepatitis E Virus Replication. Antiviral Res. 2013;100(1):98-101. PubMed PMID: 23916729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication. AU - Debing,Yannick, AU - Winton,James, AU - Neyts,Johan, AU - Dallmeier,Kai, Y1 - 2013/07/31/ PY - 2013/03/13/received PY - 2013/07/03/revised PY - 2013/07/22/accepted PY - 2013/8/7/entrez PY - 2013/8/7/pubmed PY - 2014/7/6/medline KW - Antiviral KW - Cutthroat trout virus KW - Hepatitis E virus KW - Interferon KW - Pregnancy KW - Ribavirin SP - 98 EP - 101 JF - Antiviral research JO - Antiviral Res VL - 100 IS - 1 N2 - Hepatitis E virus (HEV) is one of the most important causes of acute hepatitis worldwide. Although most infections are self-limiting, mortality is particularly high in pregnant women. Chronic infections can occur in transplant and other immune-compromised patients. Successful treatment of chronic hepatitis E has been reported with ribavirin and pegylated interferon-alpha, however severe side effects were observed. We employed the cutthroat trout virus (CTV), a non-pathogenic fish virus with remarkable similarities to HEV, as a potential surrogate for HEV and established an antiviral assay against this virus using the Chinook salmon embryo (CHSE-214) cell line. Ribavirin and the respective trout interferon were found to efficiently inhibit CTV replication. Other known broad-spectrum inhibitors of RNA virus replication such as the nucleoside analog 2'-C-methylcytidine resulted only in a moderate antiviral activity. In its natural fish host, CTV levels largely fluctuate during the reproductive cycle with the virus detected mainly during spawning. We wondered whether this aspect of CTV infection may serve as a surrogate model for the peculiar pathogenesis of HEV in pregnant women. To that end the effect of three sex steroids on in vitro CTV replication was evaluated. Whereas progesterone resulted in marked inhibition of virus replication, testosterone and 17β-estradiol stimulated viral growth. Our data thus indicate that CTV may serve as a surrogate model for HEV, both for antiviral experiments and studies on the replication biology of the Hepeviridae. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/23916729/Cutthroat_trout_virus_as_a_surrogate_in_vitro_infection_model_for_testing_inhibitors_of_hepatitis_E_virus_replication_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(13)00200-3 DB - PRIME DP - Unbound Medicine ER -