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Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay.
RNA Biol. 2013 Aug; 10(8):1291-8.RB

Abstract

The exon junction complex (EJC) participates in the regulation of many post-transcriptional steps of gene expression. EJCs are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Here, we show that two genes encoding MAGOH paralogs (referred to as MAGOH and MAGOHB) are expressed in mammals. In macrophages, the expression of MAGOHB, but not MAGOH mRNA, increases rapidly after LPS stimulation. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Furthermore, the simultaneous depletion of MAGOH and MAGOHB, but not individual depletions, impair nonsense-mediated decay in human cells. Hence, our results establish that the core composition of mammalian EJCs is more complex than previously recognized.

Authors+Show Affiliations

University of Cologne; Institute for Genetics; Cologne, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23917022

Citation

Singh, Kusum K., et al. "Two Mammalian MAGOH Genes Contribute to Exon Junction Complex Composition and Nonsense-mediated Decay." RNA Biology, vol. 10, no. 8, 2013, pp. 1291-8.
Singh KK, Wachsmuth L, Kulozik AE, et al. Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay. RNA Biol. 2013;10(8):1291-8.
Singh, K. K., Wachsmuth, L., Kulozik, A. E., & Gehring, N. H. (2013). Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay. RNA Biology, 10(8), 1291-8. https://doi.org/10.4161/rna.25827
Singh KK, et al. Two Mammalian MAGOH Genes Contribute to Exon Junction Complex Composition and Nonsense-mediated Decay. RNA Biol. 2013;10(8):1291-8. PubMed PMID: 23917022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two mammalian MAGOH genes contribute to exon junction complex composition and nonsense-mediated decay. AU - Singh,Kusum K, AU - Wachsmuth,Laurens, AU - Kulozik,Andreas E, AU - Gehring,Niels H, Y1 - 2013/07/23/ PY - 2013/8/7/entrez PY - 2013/8/7/pubmed PY - 2014/4/23/medline KW - EJC KW - MAGOHB KW - NMD KW - gene duplication KW - mago nashi homolog SP - 1291 EP - 8 JF - RNA biology JO - RNA Biol VL - 10 IS - 8 N2 - The exon junction complex (EJC) participates in the regulation of many post-transcriptional steps of gene expression. EJCs are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Here, we show that two genes encoding MAGOH paralogs (referred to as MAGOH and MAGOHB) are expressed in mammals. In macrophages, the expression of MAGOHB, but not MAGOH mRNA, increases rapidly after LPS stimulation. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Furthermore, the simultaneous depletion of MAGOH and MAGOHB, but not individual depletions, impair nonsense-mediated decay in human cells. Hence, our results establish that the core composition of mammalian EJCs is more complex than previously recognized. SN - 1555-8584 UR - https://www.unboundmedicine.com/medline/citation/23917022/Two_mammalian_MAGOH_genes_contribute_to_exon_junction_complex_composition_and_nonsense_mediated_decay_ L2 - http://www.tandfonline.com/doi/full/10.4161/rna.25827 DB - PRIME DP - Unbound Medicine ER -