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Hydrogen peroxide induced impairment of endothelial progenitor cell viability is mediated through a FoxO3a dependant mechanism.
Microvasc Res. 2013 Nov; 90:48-54.MR

Abstract

OBJECTIVES

Increased oxidative stress has been suggested to contribute to the functional impairment of endothelial progenitor cells (EPCs). The Forkhead box O transcription factors (FoxOs) are critical regulators involved in various cellular processes including cell apoptosis. Here, we investigated whether FoxOs are required in oxidative stress induced EPC apoptosis.

METHODS AND RESULTS

EPCs were cultured from cord blood derived mononuclear cells and treated with hydrogen peroxide (H2O2) for induction of oxidative stress. Incubation with H2O2 dose dependently reduced viability and increased apoptosis in EPCs. Western blotting showed that EPCs predominantly expressed FoxO3a and the expression was markedly increased upon H2O2 treatment. Transduction with adenoviral vectors expressing either a wide-type or a non-phosphorylatable, constitutively active mutant of FoxO3a led to further increased apoptosis of EPCs after H2O2 treatment. Conversely, FoxO3a silencing rescued EPCs from these H2O2 induced deleterious effects. Overexpression of FoxO3a also increased the level of the pro-apoptotic protein Bim, whereas FoxO3a silencing downregulated H2O2 induced Bim expression. Furthermore, Matrigel assay demonstrated that FoxO3a overexpression significantly impaired the tube forming ability of EPCs, whereas its silencing completely protected EPCs from H2O2 induced decrease of capillary formation.

CONCLUSIONS

These data suggest that oxidative stress induced impairment of EPC survival is mediated through a FoxO3a dependant mechanism, possibly by transcriptional regulation of Bim. Our data indicate FoxO3a as a potential therapeutic target for improvement of EPC number and function in patients with ischemic heart disease.

Authors+Show Affiliations

Department of Gerontology, Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23920411

Citation

Wang, Fei, et al. "Hydrogen Peroxide Induced Impairment of Endothelial Progenitor Cell Viability Is Mediated Through a FoxO3a Dependant Mechanism." Microvascular Research, vol. 90, 2013, pp. 48-54.
Wang F, Wang YQ, Cao Q, et al. Hydrogen peroxide induced impairment of endothelial progenitor cell viability is mediated through a FoxO3a dependant mechanism. Microvasc Res. 2013;90:48-54.
Wang, F., Wang, Y. Q., Cao, Q., Zhang, J. J., Huang, L. Y., Sang, T. T., Liu, F., & Chen, S. Y. (2013). Hydrogen peroxide induced impairment of endothelial progenitor cell viability is mediated through a FoxO3a dependant mechanism. Microvascular Research, 90, 48-54. https://doi.org/10.1016/j.mvr.2013.07.009
Wang F, et al. Hydrogen Peroxide Induced Impairment of Endothelial Progenitor Cell Viability Is Mediated Through a FoxO3a Dependant Mechanism. Microvasc Res. 2013;90:48-54. PubMed PMID: 23920411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen peroxide induced impairment of endothelial progenitor cell viability is mediated through a FoxO3a dependant mechanism. AU - Wang,Fei, AU - Wang,Yu-Qiang, AU - Cao,Qing, AU - Zhang,Jian-Jun, AU - Huang,Li-Ya, AU - Sang,Tian-Tian, AU - Liu,Fang, AU - Chen,Shu-Yan, Y1 - 2013/08/03/ PY - 2013/06/19/received PY - 2013/07/22/revised PY - 2013/07/25/accepted PY - 2013/8/8/entrez PY - 2013/8/8/pubmed PY - 2014/7/25/medline SP - 48 EP - 54 JF - Microvascular research JO - Microvasc Res VL - 90 N2 - OBJECTIVES: Increased oxidative stress has been suggested to contribute to the functional impairment of endothelial progenitor cells (EPCs). The Forkhead box O transcription factors (FoxOs) are critical regulators involved in various cellular processes including cell apoptosis. Here, we investigated whether FoxOs are required in oxidative stress induced EPC apoptosis. METHODS AND RESULTS: EPCs were cultured from cord blood derived mononuclear cells and treated with hydrogen peroxide (H2O2) for induction of oxidative stress. Incubation with H2O2 dose dependently reduced viability and increased apoptosis in EPCs. Western blotting showed that EPCs predominantly expressed FoxO3a and the expression was markedly increased upon H2O2 treatment. Transduction with adenoviral vectors expressing either a wide-type or a non-phosphorylatable, constitutively active mutant of FoxO3a led to further increased apoptosis of EPCs after H2O2 treatment. Conversely, FoxO3a silencing rescued EPCs from these H2O2 induced deleterious effects. Overexpression of FoxO3a also increased the level of the pro-apoptotic protein Bim, whereas FoxO3a silencing downregulated H2O2 induced Bim expression. Furthermore, Matrigel assay demonstrated that FoxO3a overexpression significantly impaired the tube forming ability of EPCs, whereas its silencing completely protected EPCs from H2O2 induced decrease of capillary formation. CONCLUSIONS: These data suggest that oxidative stress induced impairment of EPC survival is mediated through a FoxO3a dependant mechanism, possibly by transcriptional regulation of Bim. Our data indicate FoxO3a as a potential therapeutic target for improvement of EPC number and function in patients with ischemic heart disease. SN - 1095-9319 UR - https://www.unboundmedicine.com/medline/citation/23920411/Hydrogen_peroxide_induced_impairment_of_endothelial_progenitor_cell_viability_is_mediated_through_a_FoxO3a_dependant_mechanism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-2862(13)00111-8 DB - PRIME DP - Unbound Medicine ER -