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[Establishment and preliminary application of dengue virus envelope domain III IgG antibody capture enzyme-linked immuno-absorbent assay].
Zhonghua Yu Fang Yi Xue Za Zhi. 2013 Apr; 47(4):363-6.ZY

Abstract

OBJECTIVE

To establish a highly sensitive and specific assay to detect dengue virus (DENV) envelope protein domain III (EDIII) IgG antibody, and to explore its value in the diagnosis and seroepidemiological survey of dengue.

METHODS

The DENV EDIII IgG antibody capture ELISA was developed using the recombinant full-length DENV EDIII, which was prepared by Pichia yeast expression system as the capture antigen. The serum samples were collected from the same group of 35 DENV-1 patients of primary infection during disease period in 2006 and their follow-up phase in 2010; and the sensitivity of the assay was compared to that of the commercial Panbio DENV IgG ELISA.

RESULTS

The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from disease period and follow-up phase was 87% (20/23) and 94% (33/35), respectively; whereas the sensitivity of Panbio DENV IgG ELISA was 71% (25/35) and 0, respectively. The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from both periods was similar, without statistical significance (χ(2) = 0.946, P = 0.331). For serum samples from disease period, the sensitivity of DENV EDIII IgG ELISA was comparable with that of Panbio DENV IgG ELISA (χ(2) = 1.924, P = 0.165). However, DENV EDIII IgG ELISA demonstrated a significantly higher sensitivity than Panbio DENV IgG ELISA in detecting the serum samples from follow-up phase (χ(2) = 62.432, P = 0.000).

CONCLUSION

DENV EDIII IgG capture ELISA is highly sensitive in detecting IgG in the serum samples from either disease period or follow-up phase. This method might be a promising alternative for diagnosis and seroepidemiologic survey of dengue.

Authors+Show Affiliations

Center for Clinical Medicine Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23928645

Citation

Hu, Dong-mei, et al. "[Establishment and Preliminary Application of Dengue Virus Envelope Domain III IgG Antibody Capture Enzyme-linked Immuno-absorbent Assay]." Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], vol. 47, no. 4, 2013, pp. 363-6.
Hu DM, Cai JP, Wang DH, et al. [Establishment and preliminary application of dengue virus envelope domain III IgG antibody capture enzyme-linked immuno-absorbent assay]. Zhonghua Yu Fang Yi Xue Za Zhi. 2013;47(4):363-6.
Hu, D. M., Cai, J. P., Wang, D. H., DI, B., Qiu, L. W., Wang, Y. D., Chen, Y., Ding, X. X., & Che, X. Y. (2013). [Establishment and preliminary application of dengue virus envelope domain III IgG antibody capture enzyme-linked immuno-absorbent assay]. Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], 47(4), 363-6.
Hu DM, et al. [Establishment and Preliminary Application of Dengue Virus Envelope Domain III IgG Antibody Capture Enzyme-linked Immuno-absorbent Assay]. Zhonghua Yu Fang Yi Xue Za Zhi. 2013;47(4):363-6. PubMed PMID: 23928645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Establishment and preliminary application of dengue virus envelope domain III IgG antibody capture enzyme-linked immuno-absorbent assay]. AU - Hu,Dong-mei, AU - Cai,Jian-piao, AU - Wang,Da-hu, AU - DI,Biao, AU - Qiu,Li-wen, AU - Wang,Ya-di, AU - Chen,Yue, AU - Ding,Xi-xia, AU - Che,Xiao-yan, PY - 2013/8/10/entrez PY - 2013/8/10/pubmed PY - 2014/9/26/medline SP - 363 EP - 6 JF - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] JO - Zhonghua Yu Fang Yi Xue Za Zhi VL - 47 IS - 4 N2 - OBJECTIVE: To establish a highly sensitive and specific assay to detect dengue virus (DENV) envelope protein domain III (EDIII) IgG antibody, and to explore its value in the diagnosis and seroepidemiological survey of dengue. METHODS: The DENV EDIII IgG antibody capture ELISA was developed using the recombinant full-length DENV EDIII, which was prepared by Pichia yeast expression system as the capture antigen. The serum samples were collected from the same group of 35 DENV-1 patients of primary infection during disease period in 2006 and their follow-up phase in 2010; and the sensitivity of the assay was compared to that of the commercial Panbio DENV IgG ELISA. RESULTS: The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from disease period and follow-up phase was 87% (20/23) and 94% (33/35), respectively; whereas the sensitivity of Panbio DENV IgG ELISA was 71% (25/35) and 0, respectively. The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from both periods was similar, without statistical significance (χ(2) = 0.946, P = 0.331). For serum samples from disease period, the sensitivity of DENV EDIII IgG ELISA was comparable with that of Panbio DENV IgG ELISA (χ(2) = 1.924, P = 0.165). However, DENV EDIII IgG ELISA demonstrated a significantly higher sensitivity than Panbio DENV IgG ELISA in detecting the serum samples from follow-up phase (χ(2) = 62.432, P = 0.000). CONCLUSION: DENV EDIII IgG capture ELISA is highly sensitive in detecting IgG in the serum samples from either disease period or follow-up phase. This method might be a promising alternative for diagnosis and seroepidemiologic survey of dengue. SN - 0253-9624 UR - https://www.unboundmedicine.com/medline/citation/23928645/[Establishment_and_preliminary_application_of_dengue_virus_envelope_domain_III_IgG_antibody_capture_enzyme_linked_immuno_absorbent_assay]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0253-9624&year=2013&vol=47&issue=4&fpage=363 DB - PRIME DP - Unbound Medicine ER -