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Biodiversity and clinico-demographic characteristics of human rhinoviruses from hospitalized children with acute lower respiratory tract infections in Malaysia.
J Clin Virol. 2013 Dec; 58(4):671-7.JC

Abstract

BACKGROUND

There is accumulating evidence that human rhinovirus (HRV) causes acute lower respiratory tract infections (ALRTI). Recently, HRV-C was identified as a new species of HRV, but its spectrum of clinical disease is not well understood.

OBJECTIVES

We investigated the molecular epidemiology, demographic and clinical characteristics of HRVs among hospitalized children with ALRIs.

STUDY DESIGN

One hundred and sixty-five nasopharangeal aspirates taken from children <5 years hospitalized with ALRTIs in Serdang Hospital, Malaysia, were subject to reverse transcriptase-PCR for HRV. Phylogenetic analysis on VP4/VP2 and 5'-NCR regions was used to further characterize HRV. Other respiratory viruses were also investigated using semi-nested multiplex RT-PCR assay. Clinical parameters were analyzed between HRV, RSV and IFV-A mono-infections and between HRV species.

RESULTS

HRV was detected in 54 (33%) patients for both single (36 samples) and multiple (18 samples) infections, 61.1% (22/36) represents HRV-A strains while the remaining 14 HRV-C. Strain P51 was the first reported representative of HRV98. The majority of the single HRV cases were in the second half of infancy; HRV-C occurred among older children compared with HRV-A. HRV children were admitted significantly earlier and less febrile than RSV and IFV-A infection. HRV-C infected children were more likely to have rhonchi and vomiting as compared to HRV-A. Pneumonia was the most common discharge diagnosis followed by bronchiolitis and post-viral wheeze in HRV patients.

CONCLUSION

Our study showed high prevalence of HRVs and detection of HRV-C among hospitalized children with ALRTIs in Malaysia. Analysis of clinical parameters suggested specific features associated with HRVs infections and specific HRV groups.

Authors+Show Affiliations

Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23932333

Citation

Etemadi, Mohammad Reza, et al. "Biodiversity and Clinico-demographic Characteristics of Human Rhinoviruses From Hospitalized Children With Acute Lower Respiratory Tract Infections in Malaysia." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 58, no. 4, 2013, pp. 671-7.
Etemadi MR, Othman N, Savolainen-Kopra C, et al. Biodiversity and clinico-demographic characteristics of human rhinoviruses from hospitalized children with acute lower respiratory tract infections in Malaysia. J Clin Virol. 2013;58(4):671-7.
Etemadi, M. R., Othman, N., Savolainen-Kopra, C., Sekawi, Z., Wahab, N., & Sann, L. M. (2013). Biodiversity and clinico-demographic characteristics of human rhinoviruses from hospitalized children with acute lower respiratory tract infections in Malaysia. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 58(4), 671-7. https://doi.org/10.1016/j.jcv.2013.05.017
Etemadi MR, et al. Biodiversity and Clinico-demographic Characteristics of Human Rhinoviruses From Hospitalized Children With Acute Lower Respiratory Tract Infections in Malaysia. J Clin Virol. 2013;58(4):671-7. PubMed PMID: 23932333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biodiversity and clinico-demographic characteristics of human rhinoviruses from hospitalized children with acute lower respiratory tract infections in Malaysia. AU - Etemadi,Mohammad Reza, AU - Othman,Norlijah, AU - Savolainen-Kopra,Carita, AU - Sekawi,Zamberi, AU - Wahab,NoraAbd, AU - Sann,Lye Munn, Y1 - 2013/08/07/ PY - 2012/12/19/received PY - 2013/05/10/revised PY - 2013/05/23/accepted PY - 2013/8/13/entrez PY - 2013/8/13/pubmed PY - 2014/9/4/medline KW - Acute lower respiratory tract infections KW - Hospitalized KW - Human rhinoviruses KW - Malaysia SP - 671 EP - 7 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 58 IS - 4 N2 - BACKGROUND: There is accumulating evidence that human rhinovirus (HRV) causes acute lower respiratory tract infections (ALRTI). Recently, HRV-C was identified as a new species of HRV, but its spectrum of clinical disease is not well understood. OBJECTIVES: We investigated the molecular epidemiology, demographic and clinical characteristics of HRVs among hospitalized children with ALRIs. STUDY DESIGN: One hundred and sixty-five nasopharangeal aspirates taken from children <5 years hospitalized with ALRTIs in Serdang Hospital, Malaysia, were subject to reverse transcriptase-PCR for HRV. Phylogenetic analysis on VP4/VP2 and 5'-NCR regions was used to further characterize HRV. Other respiratory viruses were also investigated using semi-nested multiplex RT-PCR assay. Clinical parameters were analyzed between HRV, RSV and IFV-A mono-infections and between HRV species. RESULTS: HRV was detected in 54 (33%) patients for both single (36 samples) and multiple (18 samples) infections, 61.1% (22/36) represents HRV-A strains while the remaining 14 HRV-C. Strain P51 was the first reported representative of HRV98. The majority of the single HRV cases were in the second half of infancy; HRV-C occurred among older children compared with HRV-A. HRV children were admitted significantly earlier and less febrile than RSV and IFV-A infection. HRV-C infected children were more likely to have rhonchi and vomiting as compared to HRV-A. Pneumonia was the most common discharge diagnosis followed by bronchiolitis and post-viral wheeze in HRV patients. CONCLUSION: Our study showed high prevalence of HRVs and detection of HRV-C among hospitalized children with ALRTIs in Malaysia. Analysis of clinical parameters suggested specific features associated with HRVs infections and specific HRV groups. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/23932333/Biodiversity_and_clinico_demographic_characteristics_of_human_rhinoviruses_from_hospitalized_children_with_acute_lower_respiratory_tract_infections_in_Malaysia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(13)00197-2 DB - PRIME DP - Unbound Medicine ER -