Tags

Type your tag names separated by a space and hit enter

Kaempferol inhibits IL-1β-induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX-2, PGE2 and MMPs.
Int J Mol Med. 2013 Oct; 32(4):971-7.IJ

Abstract

Inflammatory cytokines, matrix metalloproteinases (MMPs) and cyclooxygenase (COX)-2 released from rheumatoid arthritis synovial fibroblasts (RASFs) are involved in the destruction of both articular bone and cartilage. Kaempferol has been reported to act as an antioxidant and anti-inflammatory agent by inhibiting nitric oxide synthase and COX enzymes. The aim of the present study was to determine the effects of kaempferol on the interleukin-1β (IL-1β)-induced proliferation of RASFs and the production of MMPs, COX and prostaglandin E2 (PGE2) by RASFs. The proliferation of the RASFs stimulated with IL-1β and treated with/without kaempferol was evaluated by CCK-8 assay. The expression of MMPs, TIMP metallopeptidase inhibitor-1 (TIMP-1), COXs, PGE2 and that of intracellular MAPK signaling molecules, including p-ERK, p-p38, p-JNK and nuclear factor-κB (NF-κB) was examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA under the conditions described above. Kaempferol inhibited the proliferation of both unstimulated and IL-1β‑stimulated RASFs, as well as the mRNA and protein expression of MMP-1, MMP-3, COX-2 and PGE2 induced by IL-1β. Kaempferol also inhibited the phosphorylation of ERK-1/2, p38 and JNK, as well as the activation of NF-κB induced by IL-1β. These results indicate that kaempferol inhibits synovial fibroblast proliferation, as well as the production of and MMPs, COX‑2 and PGE2, which is involved in articular inflammation and destruction in rheumatoid arthritis (RA). Our data suggest that kaempferol may be a novel therapeutic agent for the treatment of RA.

Authors+Show Affiliations

Department of Internal Medicine, Chonbuk National University Medical School and Research Institute of Clinical Medicine of Chonbuk National University Hospital, Chonbuk National University, Jeonju, Jeonbuk 561‑180, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23934131

Citation

Yoon, Ha-Yong, et al. "Kaempferol Inhibits IL-1β-induced Proliferation of Rheumatoid Arthritis Synovial Fibroblasts and the Production of COX-2, PGE2 and MMPs." International Journal of Molecular Medicine, vol. 32, no. 4, 2013, pp. 971-7.
Yoon HY, Lee EG, Lee H, et al. Kaempferol inhibits IL-1β-induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX-2, PGE2 and MMPs. Int J Mol Med. 2013;32(4):971-7.
Yoon, H. Y., Lee, E. G., Lee, H., Cho, I. J., Choi, Y. J., Sung, M. S., Yoo, H. G., & Yoo, W. H. (2013). Kaempferol inhibits IL-1β-induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX-2, PGE2 and MMPs. International Journal of Molecular Medicine, 32(4), 971-7. https://doi.org/10.3892/ijmm.2013.1468
Yoon HY, et al. Kaempferol Inhibits IL-1β-induced Proliferation of Rheumatoid Arthritis Synovial Fibroblasts and the Production of COX-2, PGE2 and MMPs. Int J Mol Med. 2013;32(4):971-7. PubMed PMID: 23934131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kaempferol inhibits IL-1β-induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX-2, PGE2 and MMPs. AU - Yoon,Ha-Yong, AU - Lee,Eun-Gyeong, AU - Lee,Hyun, AU - Cho,In Jin, AU - Choi,Yun Jung, AU - Sung,Myung-Soon, AU - Yoo,Han-Gyul, AU - Yoo,Wan-Hee, Y1 - 2013/08/09/ PY - 2013/05/27/received PY - 2013/08/01/accepted PY - 2013/8/13/entrez PY - 2013/8/13/pubmed PY - 2014/3/29/medline SP - 971 EP - 7 JF - International journal of molecular medicine JO - Int J Mol Med VL - 32 IS - 4 N2 - Inflammatory cytokines, matrix metalloproteinases (MMPs) and cyclooxygenase (COX)-2 released from rheumatoid arthritis synovial fibroblasts (RASFs) are involved in the destruction of both articular bone and cartilage. Kaempferol has been reported to act as an antioxidant and anti-inflammatory agent by inhibiting nitric oxide synthase and COX enzymes. The aim of the present study was to determine the effects of kaempferol on the interleukin-1β (IL-1β)-induced proliferation of RASFs and the production of MMPs, COX and prostaglandin E2 (PGE2) by RASFs. The proliferation of the RASFs stimulated with IL-1β and treated with/without kaempferol was evaluated by CCK-8 assay. The expression of MMPs, TIMP metallopeptidase inhibitor-1 (TIMP-1), COXs, PGE2 and that of intracellular MAPK signaling molecules, including p-ERK, p-p38, p-JNK and nuclear factor-κB (NF-κB) was examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA under the conditions described above. Kaempferol inhibited the proliferation of both unstimulated and IL-1β‑stimulated RASFs, as well as the mRNA and protein expression of MMP-1, MMP-3, COX-2 and PGE2 induced by IL-1β. Kaempferol also inhibited the phosphorylation of ERK-1/2, p38 and JNK, as well as the activation of NF-κB induced by IL-1β. These results indicate that kaempferol inhibits synovial fibroblast proliferation, as well as the production of and MMPs, COX‑2 and PGE2, which is involved in articular inflammation and destruction in rheumatoid arthritis (RA). Our data suggest that kaempferol may be a novel therapeutic agent for the treatment of RA. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/23934131/Kaempferol_inhibits_IL_1β_induced_proliferation_of_rheumatoid_arthritis_synovial_fibroblasts_and_the_production_of_COX_2_PGE2_and_MMPs_ L2 - http://www.spandidos-publications.com/ijmm/32/4/971 DB - PRIME DP - Unbound Medicine ER -