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A map of cochlear perilymph protein based on high-resolution two-dimensional electrophoresis.
Eur Arch Otorhinolaryngol. 1990; 247(5):271-3.EA

Abstract

For systematic characterization of cochlear perilymphatic (PL) fluid proteins and to compare the complex protein mixtures of PL fluid, cerebrospinal fluid (CSF) and blood plasma, we subjected eight postmortem PL fluid samples to two-dimensional (2D) electrophoresis. To avoid contamination of perilymph by blood and blood plasma, the samples were taken immediately after death and analyzed by keeping to certain selection criteria. When compared with CSF, PL fluid in the scala vestibule was found to have an albumin content of approximately 1-2g/l, or 10 times the level of CSF albumin. Due to the small volume of the sample obtained, it was not possible to concentrate the PL fluid. As a result, protein spots in the 2D-gel could only be detected with a highly sensitive silver stain. Visual inspection of the resulting 2D-electrophoretograms showed that most of the "CSF-specific" protein clusters were present in the PL fluid pattern.

Authors+Show Affiliations

Central Laboratory, General Hospital, Salzburg, Austria.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2393558

Citation

Arrer, E, et al. "A Map of Cochlear Perilymph Protein Based On High-resolution Two-dimensional Electrophoresis." European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated With the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, vol. 247, no. 5, 1990, pp. 271-3.
Arrer E, Oberascher G, Gibitz HJ. A map of cochlear perilymph protein based on high-resolution two-dimensional electrophoresis. Eur Arch Otorhinolaryngol. 1990;247(5):271-3.
Arrer, E., Oberascher, G., & Gibitz, H. J. (1990). A map of cochlear perilymph protein based on high-resolution two-dimensional electrophoresis. European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated With the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 247(5), 271-3.
Arrer E, Oberascher G, Gibitz HJ. A Map of Cochlear Perilymph Protein Based On High-resolution Two-dimensional Electrophoresis. Eur Arch Otorhinolaryngol. 1990;247(5):271-3. PubMed PMID: 2393558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A map of cochlear perilymph protein based on high-resolution two-dimensional electrophoresis. AU - Arrer,E, AU - Oberascher,G, AU - Gibitz,H J, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 271 EP - 3 JF - European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery JO - Eur Arch Otorhinolaryngol VL - 247 IS - 5 N2 - For systematic characterization of cochlear perilymphatic (PL) fluid proteins and to compare the complex protein mixtures of PL fluid, cerebrospinal fluid (CSF) and blood plasma, we subjected eight postmortem PL fluid samples to two-dimensional (2D) electrophoresis. To avoid contamination of perilymph by blood and blood plasma, the samples were taken immediately after death and analyzed by keeping to certain selection criteria. When compared with CSF, PL fluid in the scala vestibule was found to have an albumin content of approximately 1-2g/l, or 10 times the level of CSF albumin. Due to the small volume of the sample obtained, it was not possible to concentrate the PL fluid. As a result, protein spots in the 2D-gel could only be detected with a highly sensitive silver stain. Visual inspection of the resulting 2D-electrophoretograms showed that most of the "CSF-specific" protein clusters were present in the PL fluid pattern. SN - 0937-4477 UR - https://www.unboundmedicine.com/medline/citation/2393558/A_map_of_cochlear_perilymph_protein_based_on_high_resolution_two_dimensional_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -