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Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines.
PLoS One. 2013; 8(7):e70483.Plos

Abstract

BACKGROUND

Wheat-rye addition lines are an old topic. However, the alterations and abnormal mitotic behaviours of wheat chromosomes caused by wheat-rye monosomic addition lines are seldom reported.

METHODOLOGY/PRINCIPAL FINDINGS

Octoploid triticale was derived from common wheat T. aestivum L. 'Mianyang11'×rye S. cereale L. 'Kustro' and some progeny were obtained by the controlled backcrossing of triticale with 'Mianyang11' followed by self-fertilization. Genomic in situ hybridization (GISH) using rye genomic DNA and fluorescence in situ hybridization (FISH) using repetitive sequences pAs1 and pSc119.2 as probes were used to analyze the mitotic chromosomes of these progeny. Strong pSc119.2 FISH signals could be observed at the telomeric regions of 3DS arms in 'Mianyang11'. However, the pSc119.2 FISH signals were disappeared from the selfed progeny of 4R monosomic addition line and the changed 3D chromosomes could be transmitted to next generation stably. In one of the selfed progeny of 7R monosomic addition line, one 2D chromosome was broken and three 4A chromosomes were observed. In the selfed progeny of 6R monosomic addition line, structural variation and abnormal mitotic behaviour of 3D chromosome were detected. Additionally, 1A and 4B chromosomes were eliminated from some of the progeny of 6R monosomic addition line.

CONCLUSIONS/SIGNIFICANCE

These results indicated that single rye chromosome added to wheat might cause alterations and abnormal mitotic behaviours of wheat chromosomes and it is possible that the stress caused by single alien chromosome might be one of the factors that induced karyotype alteration of wheat.

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Authors+Show Affiliations

Fu S
State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, PR China. fushulan@sicau.edu.cn
Yang M
No affiliation info available
Fei Y
No affiliation info available
Tan F
No affiliation info available
Ren Z
No affiliation info available
Yan B
No affiliation info available
Zhang H
No affiliation info available
Tang Z
No affiliation info available

MeSH

Chromosome AberrationsChromosomes, PlantGenetic VariationHybridization, GeneticIn Situ Hybridization, FluorescenceMitosisPolyploidySecaleTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23936213

Citation

Fu, Shulan, et al. "Alterations and Abnormal Mitosis of Wheat Chromosomes Induced By Wheat-rye Monosomic Addition Lines." PloS One, vol. 8, no. 7, 2013, pp. e70483.
Fu S, Yang M, Fei Y, et al. Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines. PLoS One. 2013;8(7):e70483.
Fu, S., Yang, M., Fei, Y., Tan, F., Ren, Z., Yan, B., Zhang, H., & Tang, Z. (2013). Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines. PloS One, 8(7), e70483. https://doi.org/10.1371/journal.pone.0070483
Fu S, et al. Alterations and Abnormal Mitosis of Wheat Chromosomes Induced By Wheat-rye Monosomic Addition Lines. PLoS One. 2013;8(7):e70483. PubMed PMID: 23936213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines. AU - Fu,Shulan, AU - Yang,Manyu, AU - Fei,Yunyan, AU - Tan,Feiquan, AU - Ren,Zhenglong, AU - Yan,Benju, AU - Zhang,Huaiyu, AU - Tang,Zongxiang, Y1 - 2013/07/30/ PY - 2013/04/16/received PY - 2013/06/19/accepted PY - 2013/8/13/entrez PY - 2013/8/13/pubmed PY - 2014/4/2/medline SP - e70483 EP - e70483 JF - PloS one JO - PLoS One VL - 8 IS - 7 N2 - BACKGROUND: Wheat-rye addition lines are an old topic. However, the alterations and abnormal mitotic behaviours of wheat chromosomes caused by wheat-rye monosomic addition lines are seldom reported. METHODOLOGY/PRINCIPAL FINDINGS: Octoploid triticale was derived from common wheat T. aestivum L. 'Mianyang11'×rye S. cereale L. 'Kustro' and some progeny were obtained by the controlled backcrossing of triticale with 'Mianyang11' followed by self-fertilization. Genomic in situ hybridization (GISH) using rye genomic DNA and fluorescence in situ hybridization (FISH) using repetitive sequences pAs1 and pSc119.2 as probes were used to analyze the mitotic chromosomes of these progeny. Strong pSc119.2 FISH signals could be observed at the telomeric regions of 3DS arms in 'Mianyang11'. However, the pSc119.2 FISH signals were disappeared from the selfed progeny of 4R monosomic addition line and the changed 3D chromosomes could be transmitted to next generation stably. In one of the selfed progeny of 7R monosomic addition line, one 2D chromosome was broken and three 4A chromosomes were observed. In the selfed progeny of 6R monosomic addition line, structural variation and abnormal mitotic behaviour of 3D chromosome were detected. Additionally, 1A and 4B chromosomes were eliminated from some of the progeny of 6R monosomic addition line. CONCLUSIONS/SIGNIFICANCE: These results indicated that single rye chromosome added to wheat might cause alterations and abnormal mitotic behaviours of wheat chromosomes and it is possible that the stress caused by single alien chromosome might be one of the factors that induced karyotype alteration of wheat. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23936213/Alterations_and_abnormal_mitosis_of_wheat_chromosomes_induced_by_wheat_rye_monosomic_addition_lines_ L2 - https://dx.plos.org/10.1371/journal.pone.0070483 DB - PRIME DP - Unbound Medicine ER -