Tags

Type your tag names separated by a space and hit enter

Behaviour of four diarrheagenic Escherichia coli pathotypes on carrots and in unpasteurized carrot juice.
Lett Appl Microbiol. 2013 Dec; 57(6):540-6.LA

Abstract

The behaviours of Shiga toxin-producing Escherichia coli (STEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC) strains on raw carrots at 3 ± 1 and 30 ± 1°C, and in unpasteurized carrot juice at 3 ± 1, 12 ± 1, 20 ± 1, 30 ± 1°C and 37 ± 1°C were determined. Raw carrots were purchased in a local market. Fresh juice was obtained from raw carrots in the laboratory. On whole carrots stored at 30 ± 1 or 3 ± 1°C, no growth was observed for any of the diarrheagenic E. coli pathotype (DEPs) strains studied. After 15 days at 30 ± 1°C, the tested DEPs had decreased from an initial inoculum level of approximately 6 log colony-forming units (CFU) to approximately 3·5 log CFU on whole carrots, while at 3 ± 1°C, they decreased from approximately 2·4 log to 1·6 log CFU. All these DEPs grew in fresh carrot juice at 12 ± 1, 20 ± 1, 30 ± 1 and 37 ± 1°C, reaching counts of approximately 4·2 log, 5·8 log, 6·7 log and 7·5 log CFU ml(-1) , respectively, after 24 h. At 3 ± 1°C, the DEP growth was inhibited at least during 7 days. Thus, storage of carrot juice at unrefrigerated temperatures can result in DEP growth to levels likely to represent a risk to consumers.

SIGNIFICANCE AND IMPACT OF THE STUDY

The information presented shows the potential of Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli and enterotoxigenic E. coli strains for survival on carrots and growth in carrot juice at warmer temperatures. The information can help food processors in plants and restaurants understand the importance of the implementation of hazard analysis and critical control point (HACCP) strategies for preventing potential diarrheagenic E. coli pathotypes (DEPs) contamination and growth in carrot juice. This is the first report regarding the behaviour these DEPs on carrots and in carrot juice.

Links

Aggregator Full Text

Authors+Show Affiliations

Gómez-Aldapa CA
Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Mineral de la Reforma, Hidalgo, México.
Torres-Vitela Mdel R
No affiliation info available
Acevedo-Sandoval OA
No affiliation info available
Rangel-Vargas E
No affiliation info available
Villarruel-López A
No affiliation info available
Castro-Rosas J
No affiliation info available

MeSH

BeveragesDaucus carotaEscherichia coliFood ContaminationFood HandlingMicrobial Viability

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23941495

Citation

Gómez-Aldapa, C A., et al. "Behaviour of Four Diarrheagenic Escherichia Coli Pathotypes On Carrots and in Unpasteurized Carrot Juice." Letters in Applied Microbiology, vol. 57, no. 6, 2013, pp. 540-6.
Gómez-Aldapa CA, Torres-Vitela Mdel R, Acevedo-Sandoval OA, et al. Behaviour of four diarrheagenic Escherichia coli pathotypes on carrots and in unpasteurized carrot juice. Lett Appl Microbiol. 2013;57(6):540-6.
Gómez-Aldapa, C. A., Torres-Vitela, M. d. e. l. . R., Acevedo-Sandoval, O. A., Rangel-Vargas, E., Villarruel-López, A., & Castro-Rosas, J. (2013). Behaviour of four diarrheagenic Escherichia coli pathotypes on carrots and in unpasteurized carrot juice. Letters in Applied Microbiology, 57(6), 540-6. https://doi.org/10.1111/lam.12145
Gómez-Aldapa CA, et al. Behaviour of Four Diarrheagenic Escherichia Coli Pathotypes On Carrots and in Unpasteurized Carrot Juice. Lett Appl Microbiol. 2013;57(6):540-6. PubMed PMID: 23941495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Behaviour of four diarrheagenic Escherichia coli pathotypes on carrots and in unpasteurized carrot juice. AU - Gómez-Aldapa,C A, AU - Torres-Vitela,M Del R, AU - Acevedo-Sandoval,O A, AU - Rangel-Vargas,E, AU - Villarruel-López,A, AU - Castro-Rosas,J, Y1 - 2013/08/29/ PY - 2013/03/16/received PY - 2013/08/06/revised PY - 2013/08/07/accepted PY - 2013/8/15/entrez PY - 2013/8/15/pubmed PY - 2014/6/15/medline KW - Shiga toxin-producing E. coli KW - carrot juice KW - diarrheagenic E. coli pathotypes KW - enteroinvasive E. coli KW - enteropathogenic E. coli KW - enterotoxigenic E. coli SP - 540 EP - 6 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 57 IS - 6 N2 - UNLABELLED: The behaviours of Shiga toxin-producing Escherichia coli (STEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC) and enterotoxigenic E. coli (ETEC) strains on raw carrots at 3 ± 1 and 30 ± 1°C, and in unpasteurized carrot juice at 3 ± 1, 12 ± 1, 20 ± 1, 30 ± 1°C and 37 ± 1°C were determined. Raw carrots were purchased in a local market. Fresh juice was obtained from raw carrots in the laboratory. On whole carrots stored at 30 ± 1 or 3 ± 1°C, no growth was observed for any of the diarrheagenic E. coli pathotype (DEPs) strains studied. After 15 days at 30 ± 1°C, the tested DEPs had decreased from an initial inoculum level of approximately 6 log colony-forming units (CFU) to approximately 3·5 log CFU on whole carrots, while at 3 ± 1°C, they decreased from approximately 2·4 log to 1·6 log CFU. All these DEPs grew in fresh carrot juice at 12 ± 1, 20 ± 1, 30 ± 1 and 37 ± 1°C, reaching counts of approximately 4·2 log, 5·8 log, 6·7 log and 7·5 log CFU ml(-1) , respectively, after 24 h. At 3 ± 1°C, the DEP growth was inhibited at least during 7 days. Thus, storage of carrot juice at unrefrigerated temperatures can result in DEP growth to levels likely to represent a risk to consumers. SIGNIFICANCE AND IMPACT OF THE STUDY: The information presented shows the potential of Shiga toxin-producing Escherichia coli, enteroinvasive E. coli, enteropathogenic E. coli and enterotoxigenic E. coli strains for survival on carrots and growth in carrot juice at warmer temperatures. The information can help food processors in plants and restaurants understand the importance of the implementation of hazard analysis and critical control point (HACCP) strategies for preventing potential diarrheagenic E. coli pathotypes (DEPs) contamination and growth in carrot juice. This is the first report regarding the behaviour these DEPs on carrots and in carrot juice. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/23941495/Behaviour_of_four_diarrheagenic_Escherichia_coli_pathotypes_on_carrots_and_in_unpasteurized_carrot_juice_ L2 - https://doi.org/10.1111/lam.12145 DB - PRIME DP - Unbound Medicine ER -

Related Citations

More