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Two-dimensional electrophoresis of dog bronchoalveolar lavage fluid proteins.
Electrophoresis. 1990 Jun; 11(6):510-3.E

Abstract

Proteins of dog bronchoalveolar lavage fluid, obtained by washing the epithelial lining layer of lungs with phosphate-buffered saline, were separated by two-dimensional electrophoresis. Due to the low protein and high salt content of the bronchoalveolar lavage fluid, samples had to be concentrated and desalted. Following electrophoresis the protein spots were visualized by silver staining. Comparing the two-dimensional protein patterns of bronchoalveolar lavage fluid with that from serum, several lung-specific proteins were detected. The most prominent protein, most probably a surfactant-associated protein, showed isoforms with isoelectric points in the range of pH 4.2-4.8, and a molecular mass of 32 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol.

Authors+Show Affiliations

Gesellschaft für Strahlen- und Umweltforschung, Projekt Inhalation, Arbeitsgruppe Biochemie, Neuherberg, Federal Republic of Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2394216

Citation

Lenz, A G., et al. "Two-dimensional Electrophoresis of Dog Bronchoalveolar Lavage Fluid Proteins." Electrophoresis, vol. 11, no. 6, 1990, pp. 510-3.
Lenz AG, Meyer B, Weber H, et al. Two-dimensional electrophoresis of dog bronchoalveolar lavage fluid proteins. Electrophoresis. 1990;11(6):510-3.
Lenz, A. G., Meyer, B., Weber, H., & Maier, K. (1990). Two-dimensional electrophoresis of dog bronchoalveolar lavage fluid proteins. Electrophoresis, 11(6), 510-3.
Lenz AG, et al. Two-dimensional Electrophoresis of Dog Bronchoalveolar Lavage Fluid Proteins. Electrophoresis. 1990;11(6):510-3. PubMed PMID: 2394216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-dimensional electrophoresis of dog bronchoalveolar lavage fluid proteins. AU - Lenz,A G, AU - Meyer,B, AU - Weber,H, AU - Maier,K, PY - 1990/6/1/pubmed PY - 1990/6/1/medline PY - 1990/6/1/entrez SP - 510 EP - 3 JF - Electrophoresis JO - Electrophoresis VL - 11 IS - 6 N2 - Proteins of dog bronchoalveolar lavage fluid, obtained by washing the epithelial lining layer of lungs with phosphate-buffered saline, were separated by two-dimensional electrophoresis. Due to the low protein and high salt content of the bronchoalveolar lavage fluid, samples had to be concentrated and desalted. Following electrophoresis the protein spots were visualized by silver staining. Comparing the two-dimensional protein patterns of bronchoalveolar lavage fluid with that from serum, several lung-specific proteins were detected. The most prominent protein, most probably a surfactant-associated protein, showed isoforms with isoelectric points in the range of pH 4.2-4.8, and a molecular mass of 32 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/2394216/Two_dimensional_electrophoresis_of_dog_bronchoalveolar_lavage_fluid_proteins_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0173-0835&date=1990&volume=11&issue=6&spage=510 DB - PRIME DP - Unbound Medicine ER -