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Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt-stressed wheat seedlings.
Gene. 2013 Oct 25; 529(2):321-5.GENE

Abstract

Exogenous salicylic acid (SA) significantly improved abiotic tolerance in higher plants, and ascorbate (ASA) and glutathione (GSH) play important roles in abiotic tolerance. In this study, SA (0.5mM) markedly increased the contents of ASA and GSH in SA-treated plants during salt stress (250mM NaCl). The transcript levels of the genes encoding ASA and GSH cycle enzymes were measured using quantitative real-time PCR. The results indicated that, during salt stress, exogenous SA significantly enhanced the transcripts of glutathione peroxidase (GPX1), phospholipid hydroperoxide glutathione peroxidase (GPX2) and dehydroascorbate reductase (DHAR) genes at 12h, glutathione reductase (GR) at 24h, 48h and 72h, glutathione-S-transferase 1 (GST1), 2 (GST2), monodehydroascorbate reductase (MDHAR) and glutathione synthetase (GS) at the 48h and 72h after salt stress, respectively. The results implied that SA temporally regulated the transcript levels of the genes encoding ASA-GSH cycle enzymes, resulting in the increased contents of GSH and ASA and enhanced salt tolerance.

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Authors+Show Affiliations

Li G
The National Engineering Research Centre for Wheat, The Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China.
Peng X
No affiliation info available
Wei L
No affiliation info available
Kang G
No affiliation info available

MeSH

Ascorbic AcidGene Expression Regulation, PlantGenes, PlantGlutathioneGlutathione PeroxidaseGlutathione ReductaseGlutathione SynthaseGlutathione TransferaseNADH, NADPH OxidoreductasesOxidoreductasesSalicylic AcidSeedlingsSodium ChlorideStress, PhysiologicalTranscription, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23948081

Citation

Li, Gezi, et al. "Salicylic Acid Increases the Contents of Glutathione and Ascorbate and Temporally Regulates the Related Gene Expression in Salt-stressed Wheat Seedlings." Gene, vol. 529, no. 2, 2013, pp. 321-5.
Li G, Peng X, Wei L, et al. Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt-stressed wheat seedlings. Gene. 2013;529(2):321-5.
Li, G., Peng, X., Wei, L., & Kang, G. (2013). Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt-stressed wheat seedlings. Gene, 529(2), 321-5. https://doi.org/10.1016/j.gene.2013.07.093
Li G, et al. Salicylic Acid Increases the Contents of Glutathione and Ascorbate and Temporally Regulates the Related Gene Expression in Salt-stressed Wheat Seedlings. Gene. 2013 Oct 25;529(2):321-5. PubMed PMID: 23948081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt-stressed wheat seedlings. AU - Li,Gezi, AU - Peng,Xiaoqi, AU - Wei,Liting, AU - Kang,Guozhang, Y1 - 2013/08/13/ PY - 2013/01/29/received PY - 2013/07/22/revised PY - 2013/07/29/accepted PY - 2013/8/17/entrez PY - 2013/8/21/pubmed PY - 2013/11/13/medline KW - ASA KW - Ascorbate KW - CAT KW - DHAR KW - GPX KW - GR KW - GS KW - GSH KW - GST KW - Glutathione KW - MDA KW - MDHAR KW - POD KW - ROS KW - SA KW - SOD KW - Salicylic acid KW - Salt stress KW - Wheat KW - ascorbate KW - catalase KW - dehydroascorbate reductase KW - glutathione KW - glutathione peroxidase KW - glutathione reductase KW - glutathione synthetase KW - glutathione-S-transferase KW - malondialdehyde KW - monodehydroascorbate reductase KW - peroxidase KW - qPCR KW - quantitative real-time PCR KW - reactive oxygen species KW - salicylic acid KW - superoxide dismutases SP - 321 EP - 5 JF - Gene JO - Gene VL - 529 IS - 2 N2 - Exogenous salicylic acid (SA) significantly improved abiotic tolerance in higher plants, and ascorbate (ASA) and glutathione (GSH) play important roles in abiotic tolerance. In this study, SA (0.5mM) markedly increased the contents of ASA and GSH in SA-treated plants during salt stress (250mM NaCl). The transcript levels of the genes encoding ASA and GSH cycle enzymes were measured using quantitative real-time PCR. The results indicated that, during salt stress, exogenous SA significantly enhanced the transcripts of glutathione peroxidase (GPX1), phospholipid hydroperoxide glutathione peroxidase (GPX2) and dehydroascorbate reductase (DHAR) genes at 12h, glutathione reductase (GR) at 24h, 48h and 72h, glutathione-S-transferase 1 (GST1), 2 (GST2), monodehydroascorbate reductase (MDHAR) and glutathione synthetase (GS) at the 48h and 72h after salt stress, respectively. The results implied that SA temporally regulated the transcript levels of the genes encoding ASA-GSH cycle enzymes, resulting in the increased contents of GSH and ASA and enhanced salt tolerance. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/23948081/Salicylic_acid_increases_the_contents_of_glutathione_and_ascorbate_and_temporally_regulates_the_related_gene_expression_in_salt_stressed_wheat_seedlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(13)01001-9 DB - PRIME DP - Unbound Medicine ER -
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