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Exposure to multi-walled carbon nanotubes results in aggravation of airway inflammation and remodeling and in increased production of epithelium-derived innate cytokines in a mouse model of asthma.
Arch Toxicol 2014; 88(2):489-99AT

Abstract

With the development of nanotechnologies, the potential adverse effects of nanomaterials such as multi-walled carbon nanotubes (MWCNT) on the respiratory tract of asthmatics are questioned. Furthermore, investigations are necessary to understand how these effects might arise. In the present study, we hypothesized that epithelium-derived innate cytokines that are considered as important promoting factors in allergy may contribute to an aggravating effect of MWCNT on asthma. We investigated in the mouse the effect of MWCNT on systemic immune response and airway inflammation and remodeling induced by the most frequent allergen so far associated with asthma, house dust mite (HDM), and we examined the production of the innate cytokines thymic stromal lymphopoietin (TSLP), IL-25, IL-33, and GM-CSF. Mice exposed to HDM exhibited specific IgG1 in serum and inflammatory cell infiltration, and increased Th2 cytokine production, mucus hyperproduction, and collagen deposition in the airways when compared to naïve animals. Levels of total IgG1 and HDM-specific IgG1, influx of macrophages, eosinophils and neutrophils, production of collagen, TGF-β1, and mucus, as well as levels of IL-13, eotaxin, and TARC, were dose-dependently increased in mice exposed to HDM and MWCNT compared to HDM alone. These effects were associated with an increased production of TSLP, IL-25, IL-33, and GM-CSF in the airways. Our data demonstrate that MWCNT increase in a dose-dependent manner systemic immune response, as well as airway allergic inflammation and remodeling induced by HDM in the mouse. Our data suggest also a role for airway epithelium and innate cytokines in these effects.

Authors+Show Affiliations

Faculté de Pharmacie, Laboratoire de Conception et Application de Molécules Bioactives, CNRS, Université de Strasbourg, 74 route du Rhin, BP 60024, 67401, Illkirch Cedex, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23948970

Citation

Ronzani, Carole, et al. "Exposure to Multi-walled Carbon Nanotubes Results in Aggravation of Airway Inflammation and Remodeling and in Increased Production of Epithelium-derived Innate Cytokines in a Mouse Model of Asthma." Archives of Toxicology, vol. 88, no. 2, 2014, pp. 489-99.
Ronzani C, Casset A, Pons F. Exposure to multi-walled carbon nanotubes results in aggravation of airway inflammation and remodeling and in increased production of epithelium-derived innate cytokines in a mouse model of asthma. Arch Toxicol. 2014;88(2):489-99.
Ronzani, C., Casset, A., & Pons, F. (2014). Exposure to multi-walled carbon nanotubes results in aggravation of airway inflammation and remodeling and in increased production of epithelium-derived innate cytokines in a mouse model of asthma. Archives of Toxicology, 88(2), pp. 489-99. doi:10.1007/s00204-013-1116-3.
Ronzani C, Casset A, Pons F. Exposure to Multi-walled Carbon Nanotubes Results in Aggravation of Airway Inflammation and Remodeling and in Increased Production of Epithelium-derived Innate Cytokines in a Mouse Model of Asthma. Arch Toxicol. 2014;88(2):489-99. PubMed PMID: 23948970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure to multi-walled carbon nanotubes results in aggravation of airway inflammation and remodeling and in increased production of epithelium-derived innate cytokines in a mouse model of asthma. AU - Ronzani,Carole, AU - Casset,Anne, AU - Pons,Françoise, Y1 - 2013/08/15/ PY - 2013/06/07/received PY - 2013/08/01/accepted PY - 2013/8/17/entrez PY - 2013/8/21/pubmed PY - 2014/10/1/medline SP - 489 EP - 99 JF - Archives of toxicology JO - Arch. Toxicol. VL - 88 IS - 2 N2 - With the development of nanotechnologies, the potential adverse effects of nanomaterials such as multi-walled carbon nanotubes (MWCNT) on the respiratory tract of asthmatics are questioned. Furthermore, investigations are necessary to understand how these effects might arise. In the present study, we hypothesized that epithelium-derived innate cytokines that are considered as important promoting factors in allergy may contribute to an aggravating effect of MWCNT on asthma. We investigated in the mouse the effect of MWCNT on systemic immune response and airway inflammation and remodeling induced by the most frequent allergen so far associated with asthma, house dust mite (HDM), and we examined the production of the innate cytokines thymic stromal lymphopoietin (TSLP), IL-25, IL-33, and GM-CSF. Mice exposed to HDM exhibited specific IgG1 in serum and inflammatory cell infiltration, and increased Th2 cytokine production, mucus hyperproduction, and collagen deposition in the airways when compared to naïve animals. Levels of total IgG1 and HDM-specific IgG1, influx of macrophages, eosinophils and neutrophils, production of collagen, TGF-β1, and mucus, as well as levels of IL-13, eotaxin, and TARC, were dose-dependently increased in mice exposed to HDM and MWCNT compared to HDM alone. These effects were associated with an increased production of TSLP, IL-25, IL-33, and GM-CSF in the airways. Our data demonstrate that MWCNT increase in a dose-dependent manner systemic immune response, as well as airway allergic inflammation and remodeling induced by HDM in the mouse. Our data suggest also a role for airway epithelium and innate cytokines in these effects. SN - 1432-0738 UR - https://www.unboundmedicine.com/medline/citation/23948970/Exposure_to_multi_walled_carbon_nanotubes_results_in_aggravation_of_airway_inflammation_and_remodeling_and_in_increased_production_of_epithelium_derived_innate_cytokines_in_a_mouse_model_of_asthma_ L2 - https://dx.doi.org/10.1007/s00204-013-1116-3 DB - PRIME DP - Unbound Medicine ER -