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Oral fluid cannabinoid concentrations following controlled smoked cannabis in chronic frequent and occasional smokers.
Anal Bioanal Chem. 2013 Oct; 405(26):8451-61.AB

Abstract

Oral fluid (OF) is an alternative biological matrix for monitoring cannabis intake in drug testing, and drugged driving (DUID) programs, but OF cannabinoid test interpretation is challenging. Controlled cannabinoid administration studies provide a scientific database for interpreting cannabinoid OF tests. We compared differences in OF cannabinoid concentrations from 19 h before to 30 h after smoking a 6.8% THC cigarette in chronic frequent and occasional cannabis smokers. OF was collected with the Statsure Saliva Sampler™ OF device. 2D-GC-MS was used to quantify cannabinoids in 357 OF specimens; 65 had inadequate OF volume within 3 h after smoking. All OF specimens were THC-positive for up to 13.5 h after smoking, without significant differences between frequent and occasional smokers over 30 h. Cannabidiol (CBD) and cannabinol (CBN) had short median last detection times (2.5-4 h for CBD and 6-8 h for CBN) in both groups. THCCOOH was detected in 25 and 212 occasional and frequent smokers' OF samples, respectively. THCCOOH provided longer detection windows than THC in all frequent smokers. As THCCOOH is not present in cannabis smoke, its presence in OF minimizes the potential for false positive results from passive environmental smoke exposure, and can identify oral THC ingestion, while OF THC cannot. THC ≥ 1 μg/L, in addition to CBD ≥ 1 μg/L or CBN ≥ 1 μg/L suggested recent cannabis intake (≤13.5 h), important for DUID cases, whereas THC ≥ 1 μg/L or THC ≥ 2 μg/L cutoffs had longer detection windows (≥30 h), important for workplace testing. THCCOOH windows of detection for chronic, frequent cannabis smokers extended beyond 30 h, while they were shorter (0-24 h) for occasional cannabis smokers.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, 251 Bayview Boulevard, Baltimore, MD, 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

23954944

Citation

Anizan, Sebastien, et al. "Oral Fluid Cannabinoid Concentrations Following Controlled Smoked Cannabis in Chronic Frequent and Occasional Smokers." Analytical and Bioanalytical Chemistry, vol. 405, no. 26, 2013, pp. 8451-61.
Anizan S, Milman G, Desrosiers N, et al. Oral fluid cannabinoid concentrations following controlled smoked cannabis in chronic frequent and occasional smokers. Anal Bioanal Chem. 2013;405(26):8451-61.
Anizan, S., Milman, G., Desrosiers, N., Barnes, A. J., Gorelick, D. A., & Huestis, M. A. (2013). Oral fluid cannabinoid concentrations following controlled smoked cannabis in chronic frequent and occasional smokers. Analytical and Bioanalytical Chemistry, 405(26), 8451-61. https://doi.org/10.1007/s00216-013-7291-5
Anizan S, et al. Oral Fluid Cannabinoid Concentrations Following Controlled Smoked Cannabis in Chronic Frequent and Occasional Smokers. Anal Bioanal Chem. 2013;405(26):8451-61. PubMed PMID: 23954944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral fluid cannabinoid concentrations following controlled smoked cannabis in chronic frequent and occasional smokers. AU - Anizan,Sebastien, AU - Milman,Garry, AU - Desrosiers,Nathalie, AU - Barnes,Allan J, AU - Gorelick,David A, AU - Huestis,Marilyn A, Y1 - 2013/08/17/ PY - 2013/06/21/received PY - 2013/08/02/accepted PY - 2013/08/01/revised PY - 2013/8/20/entrez PY - 2013/8/21/pubmed PY - 2014/6/13/medline SP - 8451 EP - 61 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 405 IS - 26 N2 - Oral fluid (OF) is an alternative biological matrix for monitoring cannabis intake in drug testing, and drugged driving (DUID) programs, but OF cannabinoid test interpretation is challenging. Controlled cannabinoid administration studies provide a scientific database for interpreting cannabinoid OF tests. We compared differences in OF cannabinoid concentrations from 19 h before to 30 h after smoking a 6.8% THC cigarette in chronic frequent and occasional cannabis smokers. OF was collected with the Statsure Saliva Sampler™ OF device. 2D-GC-MS was used to quantify cannabinoids in 357 OF specimens; 65 had inadequate OF volume within 3 h after smoking. All OF specimens were THC-positive for up to 13.5 h after smoking, without significant differences between frequent and occasional smokers over 30 h. Cannabidiol (CBD) and cannabinol (CBN) had short median last detection times (2.5-4 h for CBD and 6-8 h for CBN) in both groups. THCCOOH was detected in 25 and 212 occasional and frequent smokers' OF samples, respectively. THCCOOH provided longer detection windows than THC in all frequent smokers. As THCCOOH is not present in cannabis smoke, its presence in OF minimizes the potential for false positive results from passive environmental smoke exposure, and can identify oral THC ingestion, while OF THC cannot. THC ≥ 1 μg/L, in addition to CBD ≥ 1 μg/L or CBN ≥ 1 μg/L suggested recent cannabis intake (≤13.5 h), important for DUID cases, whereas THC ≥ 1 μg/L or THC ≥ 2 μg/L cutoffs had longer detection windows (≥30 h), important for workplace testing. THCCOOH windows of detection for chronic, frequent cannabis smokers extended beyond 30 h, while they were shorter (0-24 h) for occasional cannabis smokers. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/23954944/Oral_fluid_cannabinoid_concentrations_following_controlled_smoked_cannabis_in_chronic_frequent_and_occasional_smokers_ L2 - https://dx.doi.org/10.1007/s00216-013-7291-5 DB - PRIME DP - Unbound Medicine ER -