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Identification and characterization of a gene encoding a UBX domain-containing protein in the migratory locust, Locusta migratoria manilensis.
Insect Sci. 2013 Aug; 20(4):497-504.IS

Abstract

Ubiquitin regulatory X (UBX) domain-containing proteins are believed to function as cofactors for p97/CDC48, an adenosine triphosphatase shown to be involved in multiple cellular processes. In the present study, a full-length complementary DNA (cDNA) of UBX domain-containing gene, termed LmUBX1, was cloned from Locusta migratoria manilensis and characterized, using random amplification of cDNA ends polymerase chain reaction (RACE PCR), sequence analysis and quantitative real-time PCR. LmUBX1, 1 600 bp in length, is predicted to encode a 446-amino acid protein with a predicted molecular weight of 51.18 kDa that contains a central PUB domain and a carboxy-terminal UBX domain. Homology analysis revealed that LmUBX1 has higher similarity to the known UBX domain-containing proteins from insects than from other species. Moreover, based on sequence characteristics and phylogenetic relationships, it is suggested that LmUBX1 can be classified into the UBXD1 subfamily. Expression analysis founded that LmUBX1 exhibited significant expression variations at different developmental stages and in different tissues, suggesting that the expression of LmUBX1 was highly regulated. Interestingly, its messenger RNA transcript was more abundant in ovary and testis than in other tissues examined, suggesting that it may have more important roles in the reproductive system. In addition, LmUBX1 was differentially expressed in gregarious and solitary locusts and was significantly up-regulated in third and fifth instars of gregarious locusts, implying that LmUBX1 was also likely involved in the phase polyphenisms in L. migratoria manilensis. To our knowledge, this is the first report of cloning of a full-length cDNA of UBX domain-containing gene from L. migratoria manilensis.

Authors+Show Affiliations

Institute of Entomology and Molecular Biology, Chongqing Key Laboratory of Animal Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23955945

Citation

He, Zheng-Bo, et al. "Identification and Characterization of a Gene Encoding a UBX Domain-containing Protein in the Migratory Locust, Locusta Migratoria Manilensis." Insect Science, vol. 20, no. 4, 2013, pp. 497-504.
He ZB, Xie Y, Si FL, et al. Identification and characterization of a gene encoding a UBX domain-containing protein in the migratory locust, Locusta migratoria manilensis. Insect Sci. 2013;20(4):497-504.
He, Z. B., Xie, Y., Si, F. L., & Chen, B. (2013). Identification and characterization of a gene encoding a UBX domain-containing protein in the migratory locust, Locusta migratoria manilensis. Insect Science, 20(4), 497-504. https://doi.org/10.1111/j.1744-7917.2012.01548.x
He ZB, et al. Identification and Characterization of a Gene Encoding a UBX Domain-containing Protein in the Migratory Locust, Locusta Migratoria Manilensis. Insect Sci. 2013;20(4):497-504. PubMed PMID: 23955945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of a gene encoding a UBX domain-containing protein in the migratory locust, Locusta migratoria manilensis. AU - He,Zheng-Bo, AU - Xie,Yu, AU - Si,Feng-Ling, AU - Chen,Bin, Y1 - 2012/10/23/ PY - 2012/04/16/accepted PY - 2013/8/20/entrez PY - 2013/8/21/pubmed PY - 2014/6/24/medline KW - Locusta migratoria manilensis KW - UBX domain-containing protein KW - cloning KW - expression SP - 497 EP - 504 JF - Insect science JO - Insect Sci VL - 20 IS - 4 N2 - Ubiquitin regulatory X (UBX) domain-containing proteins are believed to function as cofactors for p97/CDC48, an adenosine triphosphatase shown to be involved in multiple cellular processes. In the present study, a full-length complementary DNA (cDNA) of UBX domain-containing gene, termed LmUBX1, was cloned from Locusta migratoria manilensis and characterized, using random amplification of cDNA ends polymerase chain reaction (RACE PCR), sequence analysis and quantitative real-time PCR. LmUBX1, 1 600 bp in length, is predicted to encode a 446-amino acid protein with a predicted molecular weight of 51.18 kDa that contains a central PUB domain and a carboxy-terminal UBX domain. Homology analysis revealed that LmUBX1 has higher similarity to the known UBX domain-containing proteins from insects than from other species. Moreover, based on sequence characteristics and phylogenetic relationships, it is suggested that LmUBX1 can be classified into the UBXD1 subfamily. Expression analysis founded that LmUBX1 exhibited significant expression variations at different developmental stages and in different tissues, suggesting that the expression of LmUBX1 was highly regulated. Interestingly, its messenger RNA transcript was more abundant in ovary and testis than in other tissues examined, suggesting that it may have more important roles in the reproductive system. In addition, LmUBX1 was differentially expressed in gregarious and solitary locusts and was significantly up-regulated in third and fifth instars of gregarious locusts, implying that LmUBX1 was also likely involved in the phase polyphenisms in L. migratoria manilensis. To our knowledge, this is the first report of cloning of a full-length cDNA of UBX domain-containing gene from L. migratoria manilensis. SN - 1744-7917 UR - https://www.unboundmedicine.com/medline/citation/23955945/Identification_and_characterization_of_a_gene_encoding_a_UBX_domain_containing_protein_in_the_migratory_locust_Locusta_migratoria_manilensis_ L2 - https://doi.org/10.1111/j.1744-7917.2012.01548.x DB - PRIME DP - Unbound Medicine ER -