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Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity.
Plant Cell Environ. 2014 Mar; 37(3):696-706.PC

Abstract

Plants overexpressing the RNA-binding protein AtGRP7 (AtGRP7-ox plants) constitutively express the PR-1 (PATHOGENESIS-RELATED-1), PR-2 and PR-5 transcripts associated with salicylic acid (SA)-mediated immunity and show enhanced resistance against Pseudomonas syringae pv. tomato (Pto) DC3000. Here, we investigated whether the function of AtGRP7 in plant immunity depends on SA. Endogenous SA was elevated fivefold in AtGRP7-ox plants. The elevated PR-1, PR-2 and PR-5 levels were eliminated upon expression of the salicylate hydroxylase nahG in AtGRP7-ox plants and elevated PR-1 levels were suppressed by sid (salicylic acid deficient) 2-1 that is impaired in SA biosynthesis. RNA immunoprecipitation showed that AtGRP7 does not bind the PR-1 transcript in vivo, whereas it binds PDF1.2. Constitutive or inducible AtGRP7 overexpression increases PR-1 promoter activity, indicating that AtGRP7 affects PR-1 transcription. In line with this, the effect of AtGRP7 on PR-1 is suppressed by npr (non-expressor of PR genes) 1. Whereas AtGRP7-ox plants restricted growth of Pto DC3000 compared with wild type (wt), sid2-1 AtGRP7-ox plants allowed more growth than AtGRP7-ox plants. Furthermore, we show an enhanced hypersensitive response triggered by avirulent Pto DC3000 (AvrRpt2) in AtGRP7-ox compared with wt. In sid2-1 AtGRP7-ox, an intermediate phenotype was observed. Thus, AtGRP7 has both SA-dependent and SA-independent effects on plant immunity.

Authors+Show Affiliations

Department of Molecular Cell Physiology, Bielefeld University, Universitätsstraβe 25, D-33615, Bielefeld, Germany; Institute for Genome Research and Systems Biology, CeBiTec, Bielefeld University, Universitätsstraβe 25, D-33615, Bielefeld, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23961939

Citation

Hackmann, Christian, et al. "Salicylic Acid-dependent and -independent Impact of an RNA-binding Protein On Plant Immunity." Plant, Cell & Environment, vol. 37, no. 3, 2014, pp. 696-706.
Hackmann C, Korneli C, Kutyniok M, et al. Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity. Plant Cell Environ. 2014;37(3):696-706.
Hackmann, C., Korneli, C., Kutyniok, M., Köster, T., Wiedenlübbert, M., Müller, C., & Staiger, D. (2014). Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity. Plant, Cell & Environment, 37(3), 696-706. https://doi.org/10.1111/pce.12188
Hackmann C, et al. Salicylic Acid-dependent and -independent Impact of an RNA-binding Protein On Plant Immunity. Plant Cell Environ. 2014;37(3):696-706. PubMed PMID: 23961939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salicylic acid-dependent and -independent impact of an RNA-binding protein on plant immunity. AU - Hackmann,Christian, AU - Korneli,Christin, AU - Kutyniok,Magdalene, AU - Köster,Tino, AU - Wiedenlübbert,Matthias, AU - Müller,Caroline, AU - Staiger,Dorothee, Y1 - 2013/09/22/ PY - 2012/12/04/received PY - 2013/08/09/revised PY - 2013/08/15/accepted PY - 2013/8/22/entrez PY - 2013/8/22/pubmed PY - 2014/10/29/medline KW - NPR1 KW - glycine-rich RNA-binding protein KW - pathogenesis-related genes SP - 696 EP - 706 JF - Plant, cell & environment JO - Plant Cell Environ. VL - 37 IS - 3 N2 - Plants overexpressing the RNA-binding protein AtGRP7 (AtGRP7-ox plants) constitutively express the PR-1 (PATHOGENESIS-RELATED-1), PR-2 and PR-5 transcripts associated with salicylic acid (SA)-mediated immunity and show enhanced resistance against Pseudomonas syringae pv. tomato (Pto) DC3000. Here, we investigated whether the function of AtGRP7 in plant immunity depends on SA. Endogenous SA was elevated fivefold in AtGRP7-ox plants. The elevated PR-1, PR-2 and PR-5 levels were eliminated upon expression of the salicylate hydroxylase nahG in AtGRP7-ox plants and elevated PR-1 levels were suppressed by sid (salicylic acid deficient) 2-1 that is impaired in SA biosynthesis. RNA immunoprecipitation showed that AtGRP7 does not bind the PR-1 transcript in vivo, whereas it binds PDF1.2. Constitutive or inducible AtGRP7 overexpression increases PR-1 promoter activity, indicating that AtGRP7 affects PR-1 transcription. In line with this, the effect of AtGRP7 on PR-1 is suppressed by npr (non-expressor of PR genes) 1. Whereas AtGRP7-ox plants restricted growth of Pto DC3000 compared with wild type (wt), sid2-1 AtGRP7-ox plants allowed more growth than AtGRP7-ox plants. Furthermore, we show an enhanced hypersensitive response triggered by avirulent Pto DC3000 (AvrRpt2) in AtGRP7-ox compared with wt. In sid2-1 AtGRP7-ox, an intermediate phenotype was observed. Thus, AtGRP7 has both SA-dependent and SA-independent effects on plant immunity. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/23961939/Salicylic_acid_dependent_and__independent_impact_of_an_RNA_binding_protein_on_plant_immunity_ L2 - https://doi.org/10.1111/pce.12188 DB - PRIME DP - Unbound Medicine ER -