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Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR.
PLoS One. 2013; 8(8):e71647.Plos

Abstract

Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.

Authors+Show Affiliations

Global and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia. erin.price@menzies.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23967229

Citation

Price, Erin P., et al. "Accurate and Rapid Identification of the Burkholderia Pseudomallei Near-neighbour, Burkholderia Ubonensis, Using Real-time PCR." PloS One, vol. 8, no. 8, 2013, pp. e71647.
Price EP, Sarovich DS, Webb JR, et al. Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR. PLoS One. 2013;8(8):e71647.
Price, E. P., Sarovich, D. S., Webb, J. R., Ginther, J. L., Mayo, M., Cook, J. M., Seymour, M. L., Kaestli, M., Theobald, V., Hall, C. M., Busch, J. D., Foster, J. T., Keim, P., Wagner, D. M., Tuanyok, A., Pearson, T., & Currie, B. J. (2013). Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR. PloS One, 8(8), e71647. https://doi.org/10.1371/journal.pone.0071647
Price EP, et al. Accurate and Rapid Identification of the Burkholderia Pseudomallei Near-neighbour, Burkholderia Ubonensis, Using Real-time PCR. PLoS One. 2013;8(8):e71647. PubMed PMID: 23967229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR. AU - Price,Erin P, AU - Sarovich,Derek S, AU - Webb,Jessica R, AU - Ginther,Jennifer L, AU - Mayo,Mark, AU - Cook,James M, AU - Seymour,Meagan L, AU - Kaestli,Mirjam, AU - Theobald,Vanessa, AU - Hall,Carina M, AU - Busch,Joseph D, AU - Foster,Jeffrey T, AU - Keim,Paul, AU - Wagner,David M, AU - Tuanyok,Apichai, AU - Pearson,Talima, AU - Currie,Bart J, Y1 - 2013/08/13/ PY - 2013/05/02/received PY - 2013/07/02/accepted PY - 2013/8/23/entrez PY - 2013/8/24/pubmed PY - 2014/5/16/medline SP - e71647 EP - e71647 JF - PloS one JO - PLoS One VL - 8 IS - 8 N2 - Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23967229/Accurate_and_rapid_identification_of_the_Burkholderia_pseudomallei_near_neighbour_Burkholderia_ubonensis_using_real_time_PCR_ L2 - https://dx.plos.org/10.1371/journal.pone.0071647 DB - PRIME DP - Unbound Medicine ER -