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Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR.PLoS One. 2013; 8(8):e71647.Plos
Abstract
Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.
Links
MeSH
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Language
eng
PubMed ID
23967229
Citation
Price, Erin P., et al. "Accurate and Rapid Identification of the Burkholderia Pseudomallei Near-neighbour, Burkholderia Ubonensis, Using Real-time PCR." PloS One, vol. 8, no. 8, 2013, pp. e71647.
Price EP, Sarovich DS, Webb JR, et al. Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR. PLoS One. 2013;8(8):e71647.
Price, E. P., Sarovich, D. S., Webb, J. R., Ginther, J. L., Mayo, M., Cook, J. M., Seymour, M. L., Kaestli, M., Theobald, V., Hall, C. M., Busch, J. D., Foster, J. T., Keim, P., Wagner, D. M., Tuanyok, A., Pearson, T., & Currie, B. J. (2013). Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR. PloS One, 8(8), e71647. https://doi.org/10.1371/journal.pone.0071647
Price EP, et al. Accurate and Rapid Identification of the Burkholderia Pseudomallei Near-neighbour, Burkholderia Ubonensis, Using Real-time PCR. PLoS One. 2013;8(8):e71647. PubMed PMID: 23967229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Accurate and rapid identification of the Burkholderia pseudomallei near-neighbour, Burkholderia ubonensis, using real-time PCR.
AU - Price,Erin P,
AU - Sarovich,Derek S,
AU - Webb,Jessica R,
AU - Ginther,Jennifer L,
AU - Mayo,Mark,
AU - Cook,James M,
AU - Seymour,Meagan L,
AU - Kaestli,Mirjam,
AU - Theobald,Vanessa,
AU - Hall,Carina M,
AU - Busch,Joseph D,
AU - Foster,Jeffrey T,
AU - Keim,Paul,
AU - Wagner,David M,
AU - Tuanyok,Apichai,
AU - Pearson,Talima,
AU - Currie,Bart J,
Y1 - 2013/08/13/
PY - 2013/05/02/received
PY - 2013/07/02/accepted
PY - 2013/8/23/entrez
PY - 2013/8/24/pubmed
PY - 2014/5/16/medline
SP - e71647
EP - e71647
JF - PloS one
JO - PLoS One
VL - 8
IS - 8
N2 - Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.
SN - 1932-6203
UR - https://www.unboundmedicine.com/medline/citation/23967229/Accurate_and_rapid_identification_of_the_Burkholderia_pseudomallei_near_neighbour_Burkholderia_ubonensis_using_real_time_PCR_
L2 - https://dx.plos.org/10.1371/journal.pone.0071647
DB - PRIME
DP - Unbound Medicine
ER -
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