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Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin.
Dev Biol. 2014 Jan 15; 385(2):366-79.DB

Abstract

Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and diaphragm of adult mice, we have been using flow cytometry in combination with myogenic-specific Cre-loxP lineage marking for cell isolation. While analyzing cells from the EOM of mice that harbor Myf5(Cre)-driven GFP expression, we identified in addition to the expected GFP(+) myogenic cells (presumably satellite cells), a second dominant GFP(+) population distinguished as being Sca1(+), non-myogenic, and exhibiting a fibro/adipogenic potential. This unexpected population was not only unique to EOM compared to the other muscles but also specific to the Myf5(Cre)-driven reporter when compared to the MyoD(Cre) driver. Histological studies of periocular tissue preparations demonstrated the presence of Myf5(Cre)-driven GFP(+) cells in connective tissue locations adjacent to the muscle masses, including cells in the vasculature wall. These vasculature-associated GFP(+) cells were further identified as mural cells based on the presence of the specific XLacZ4 transgene. Unlike the EOM satellite cells that originate from a Pax3-negative lineage, these non-myogenic Myf5(Cre)-driven GFP(+) cells appear to be related to cells of a Pax3-expressing origin, presumably derived from the neural crest. In all, our lineage tracing based on multiple reporter lines has demonstrated that regardless of common ancestral expression of Myf5, there is a clear distinction between periocular myogenic and non-myogenic cell lineages according to their mutually exclusive antecedence of MyoD and Pax3 gene activity.

Authors+Show Affiliations

Department of Biological Structure, University of Washington School of Medicine, Seattle, WA 98195, USA.Department of Biological Structure, University of Washington School of Medicine, Seattle, WA 98195, USA.Department of Biological Structure, University of Washington School of Medicine, Seattle, WA 98195, USA. Electronic address: reuveni@uw.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23969310

Citation

Stuelsatz, Pascal, et al. "Ancestral Myf5 Gene Activity in Periocular Connective Tissue Identifies a Subset of Fibro/adipogenic Progenitors but Does Not Connote a Myogenic Origin." Developmental Biology, vol. 385, no. 2, 2014, pp. 366-79.
Stuelsatz P, Shearer A, Yablonka-Reuveni Z. Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin. Dev Biol. 2014;385(2):366-79.
Stuelsatz, P., Shearer, A., & Yablonka-Reuveni, Z. (2014). Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin. Developmental Biology, 385(2), 366-79. https://doi.org/10.1016/j.ydbio.2013.08.010
Stuelsatz P, Shearer A, Yablonka-Reuveni Z. Ancestral Myf5 Gene Activity in Periocular Connective Tissue Identifies a Subset of Fibro/adipogenic Progenitors but Does Not Connote a Myogenic Origin. Dev Biol. 2014 Jan 15;385(2):366-79. PubMed PMID: 23969310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ancestral Myf5 gene activity in periocular connective tissue identifies a subset of fibro/adipogenic progenitors but does not connote a myogenic origin. AU - Stuelsatz,Pascal, AU - Shearer,Andrew, AU - Yablonka-Reuveni,Zipora, Y1 - 2013/08/19/ PY - 2013/05/16/received PY - 2013/07/21/revised PY - 2013/08/13/accepted PY - 2013/8/24/entrez PY - 2013/8/24/pubmed PY - 2014/3/7/medline KW - Extraocular muscles KW - Fibro/adipogenic progenitors KW - Myf5 KW - MyoD KW - Pax3 KW - Pericytes and vascular smooth muscle cells KW - Satellite cells KW - Sca-1 KW - Wnt1 KW - XLacZ4 SP - 366 EP - 79 JF - Developmental biology JO - Dev Biol VL - 385 IS - 2 N2 - Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and diaphragm of adult mice, we have been using flow cytometry in combination with myogenic-specific Cre-loxP lineage marking for cell isolation. While analyzing cells from the EOM of mice that harbor Myf5(Cre)-driven GFP expression, we identified in addition to the expected GFP(+) myogenic cells (presumably satellite cells), a second dominant GFP(+) population distinguished as being Sca1(+), non-myogenic, and exhibiting a fibro/adipogenic potential. This unexpected population was not only unique to EOM compared to the other muscles but also specific to the Myf5(Cre)-driven reporter when compared to the MyoD(Cre) driver. Histological studies of periocular tissue preparations demonstrated the presence of Myf5(Cre)-driven GFP(+) cells in connective tissue locations adjacent to the muscle masses, including cells in the vasculature wall. These vasculature-associated GFP(+) cells were further identified as mural cells based on the presence of the specific XLacZ4 transgene. Unlike the EOM satellite cells that originate from a Pax3-negative lineage, these non-myogenic Myf5(Cre)-driven GFP(+) cells appear to be related to cells of a Pax3-expressing origin, presumably derived from the neural crest. In all, our lineage tracing based on multiple reporter lines has demonstrated that regardless of common ancestral expression of Myf5, there is a clear distinction between periocular myogenic and non-myogenic cell lineages according to their mutually exclusive antecedence of MyoD and Pax3 gene activity. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/23969310/Ancestral_Myf5_gene_activity_in_periocular_connective_tissue_identifies_a_subset_of_fibro/adipogenic_progenitors_but_does_not_connote_a_myogenic_origin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(13)00422-3 DB - PRIME DP - Unbound Medicine ER -