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Prospective comparison of the detection rates of human enterovirus and parechovirus RT-qPCR and viral culture in different pediatric specimens.
J Clin Virol. 2013 Oct; 58(2):449-54.JC

Abstract

BACKGROUND

Reverse-transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) has become the gold standard for the diagnosis of human enterovirus (EV) and parechovirus (HPeV) infections. The detection rate of RT-qPCR in different pediatric body specimens has not been compared prospectively in a multicentre study.

OBJECTIVES

This study compared the diagnostic detection rates of EV and HPeV RT-qPCR and viral culture in different specimens (feces, nasopharynx, blood, urine and cerebrospinal fluid (CSF)) of pediatric patients.

STUDY DESIGN

This prospective, multicenter study performed an EV and HPeV RT-qPCR on nasopharynx, blood, urine, feces and CSF specimens and a viral culture on nasopharynx, feces and CSF specimens in symptomatic children<16 years.

RESULTS

Of 285 included children EV was detected in 140 (49%) and HPeV in 44 (15%) children. Both EV and HPeV RT-qPCR had a higher sensitivity and negative predictive value than EV and HPeV viral culture, respectively. EV and HPeV RT-qPCR in feces specimen had the highest sensitivity (99.2% and 95.1%) of all specimens. Pooling results of specimens increased the detection rate for both viruses.

CONCLUSION

Of all specimens, RT-qPCR in feces had the highest detection rate for both EV and HPeV in symptomatic pediatric patients. An EV was detected in all EV positive patients if a RT-qPCR was performed on both feces and CSF specimens or in both feces and urine specimens. HPeV was detected in all HPeV positive patients if a RT-qPCR was performed on both feces and CSF specimens, feces and nasopharynx specimens or CSF and nasopharynx specimens.

Authors+Show Affiliations

Department of Pediatrics, St. Elisabeth Hospital, Tilburg, The Netherlands; Department of Pediatrics, Maastricht University Medical Centre, Maastricht, The Netherlands. Electronic address: stephanie_de_crom@hotmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study

Language

eng

PubMed ID

23973350

Citation

de Crom, S C M., et al. "Prospective Comparison of the Detection Rates of Human Enterovirus and Parechovirus RT-qPCR and Viral Culture in Different Pediatric Specimens." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 58, no. 2, 2013, pp. 449-54.
de Crom SC, Obihara CC, de Moor RA, et al. Prospective comparison of the detection rates of human enterovirus and parechovirus RT-qPCR and viral culture in different pediatric specimens. J Clin Virol. 2013;58(2):449-54.
de Crom, S. C., Obihara, C. C., de Moor, R. A., Veldkamp, E. J., van Furth, A. M., & Rossen, J. W. (2013). Prospective comparison of the detection rates of human enterovirus and parechovirus RT-qPCR and viral culture in different pediatric specimens. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 58(2), 449-54. https://doi.org/10.1016/j.jcv.2013.07.017
de Crom SC, et al. Prospective Comparison of the Detection Rates of Human Enterovirus and Parechovirus RT-qPCR and Viral Culture in Different Pediatric Specimens. J Clin Virol. 2013;58(2):449-54. PubMed PMID: 23973350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prospective comparison of the detection rates of human enterovirus and parechovirus RT-qPCR and viral culture in different pediatric specimens. AU - de Crom,S C M, AU - Obihara,C C, AU - de Moor,R A, AU - Veldkamp,E J M, AU - van Furth,A M, AU - Rossen,J W A, Y1 - 2013/08/21/ PY - 2013/02/28/received PY - 2013/06/14/revised PY - 2013/07/23/accepted PY - 2013/8/27/entrez PY - 2013/8/27/pubmed PY - 2014/4/20/medline KW - CSF KW - EV KW - Enterovirus KW - HEp-2 KW - HPeV KW - Human parechovirus KW - NPV KW - PPV KW - Pediatric KW - RNA KW - RT-qPCR KW - Viral culture KW - cerebrospinal fluid KW - cynomolgus monkey kidney KW - human epidermoid cancer KW - human non-polio enterovirus KW - human parechovirus KW - negative predictive value KW - positive predictive value KW - reverse-transcriptase real time quantitative polymerase chain reaction KW - ribonucleic acid KW - tMK SP - 449 EP - 54 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 58 IS - 2 N2 - BACKGROUND: Reverse-transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) has become the gold standard for the diagnosis of human enterovirus (EV) and parechovirus (HPeV) infections. The detection rate of RT-qPCR in different pediatric body specimens has not been compared prospectively in a multicentre study. OBJECTIVES: This study compared the diagnostic detection rates of EV and HPeV RT-qPCR and viral culture in different specimens (feces, nasopharynx, blood, urine and cerebrospinal fluid (CSF)) of pediatric patients. STUDY DESIGN: This prospective, multicenter study performed an EV and HPeV RT-qPCR on nasopharynx, blood, urine, feces and CSF specimens and a viral culture on nasopharynx, feces and CSF specimens in symptomatic children<16 years. RESULTS: Of 285 included children EV was detected in 140 (49%) and HPeV in 44 (15%) children. Both EV and HPeV RT-qPCR had a higher sensitivity and negative predictive value than EV and HPeV viral culture, respectively. EV and HPeV RT-qPCR in feces specimen had the highest sensitivity (99.2% and 95.1%) of all specimens. Pooling results of specimens increased the detection rate for both viruses. CONCLUSION: Of all specimens, RT-qPCR in feces had the highest detection rate for both EV and HPeV in symptomatic pediatric patients. An EV was detected in all EV positive patients if a RT-qPCR was performed on both feces and CSF specimens or in both feces and urine specimens. HPeV was detected in all HPeV positive patients if a RT-qPCR was performed on both feces and CSF specimens, feces and nasopharynx specimens or CSF and nasopharynx specimens. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/23973350/Prospective_comparison_of_the_detection_rates_of_human_enterovirus_and_parechovirus_RT_qPCR_and_viral_culture_in_different_pediatric_specimens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(13)00317-X DB - PRIME DP - Unbound Medicine ER -