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Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD.
J Pharm Biomed Anal. 2013 Nov; 85:295-304.JP

Abstract

A reliable method based on high performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) was developed for the identification of phenolic acids and flavonoids in Apocynum venetum L. leaves and its adulterant, Pocynum hendersonii (Hook. f.) Woodson leaves. A total of 21 compounds were identified or tentatively identified, including 4 phenolic acids and 17 flavonoids. 3-O-caffeoylquinic acid (3-CQA) and caffeic acid were detected for the first time in A. venetum leaves; 4-O-caffeoylquinic acid (4-CQA), 3-CQA, caffeic acid, quercetin-3-O-(6"-O-malonyl)-galactoside, quercetin-3-O-(6"-O-malonyl)-glucoside, kaempferol-3-O-(6"-O-malonyl)-glucoside, kaempferol-3-O-(6"-O-acetyl)-glucoside, and kaempferol-3-O-dihexoside were detected for the first time in P. hendersonii leaves. Cluster analysis was employed to analyze 24 batches of A. venetum leaves and 5 batches of P. hendersonii leaves collected from various regions in China. The analysis, which was based on the 21 compounds, indicated that profiles of these compounds were distinct between the two species, and among A. venetum leaf samples from different origins. 18 of these 21 compounds were selected as the markers and simultaneously analyzed by HPLC-DAD for the first time. The quantitative analytical method was validated and subsequently applied to the comprehensive quality evaluation of 24 batches of A. venetum leaves.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. anhaijuan0123@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23973760

Citation

An, Haijuan, et al. "Simultaneous Qualitative and Quantitative Analysis of Phenolic Acids and Flavonoids for the Quality Control of Apocynum Venetum L. Leaves By HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD." Journal of Pharmaceutical and Biomedical Analysis, vol. 85, 2013, pp. 295-304.
An H, Wang H, Lan Y, et al. Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD. J Pharm Biomed Anal. 2013;85:295-304.
An, H., Wang, H., Lan, Y., Hashi, Y., & Chen, S. (2013). Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD. Journal of Pharmaceutical and Biomedical Analysis, 85, 295-304. https://doi.org/10.1016/j.jpba.2013.07.005
An H, et al. Simultaneous Qualitative and Quantitative Analysis of Phenolic Acids and Flavonoids for the Quality Control of Apocynum Venetum L. Leaves By HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD. J Pharm Biomed Anal. 2013;85:295-304. PubMed PMID: 23973760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous qualitative and quantitative analysis of phenolic acids and flavonoids for the quality control of Apocynum venetum L. leaves by HPLC-DAD-ESI-IT-TOF-MS and HPLC-DAD. AU - An,Haijuan, AU - Wang,Hong, AU - Lan,Yuexiang, AU - Hashi,Yuki, AU - Chen,Shizhong, Y1 - 2013/07/19/ PY - 2013/02/20/received PY - 2013/07/07/revised PY - 2013/07/08/accepted PY - 2013/8/27/entrez PY - 2013/8/27/pubmed PY - 2014/5/20/medline KW - Apocynum venetum L. leaves KW - Cluster analysis KW - Flavonoids KW - HPLC–DAD–ESI–IT–TOF–MS KW - Phenolic acids KW - Quantitative determination SP - 295 EP - 304 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 85 N2 - A reliable method based on high performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) was developed for the identification of phenolic acids and flavonoids in Apocynum venetum L. leaves and its adulterant, Pocynum hendersonii (Hook. f.) Woodson leaves. A total of 21 compounds were identified or tentatively identified, including 4 phenolic acids and 17 flavonoids. 3-O-caffeoylquinic acid (3-CQA) and caffeic acid were detected for the first time in A. venetum leaves; 4-O-caffeoylquinic acid (4-CQA), 3-CQA, caffeic acid, quercetin-3-O-(6"-O-malonyl)-galactoside, quercetin-3-O-(6"-O-malonyl)-glucoside, kaempferol-3-O-(6"-O-malonyl)-glucoside, kaempferol-3-O-(6"-O-acetyl)-glucoside, and kaempferol-3-O-dihexoside were detected for the first time in P. hendersonii leaves. Cluster analysis was employed to analyze 24 batches of A. venetum leaves and 5 batches of P. hendersonii leaves collected from various regions in China. The analysis, which was based on the 21 compounds, indicated that profiles of these compounds were distinct between the two species, and among A. venetum leaf samples from different origins. 18 of these 21 compounds were selected as the markers and simultaneously analyzed by HPLC-DAD for the first time. The quantitative analytical method was validated and subsequently applied to the comprehensive quality evaluation of 24 batches of A. venetum leaves. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/23973760/Simultaneous_qualitative_and_quantitative_analysis_of_phenolic_acids_and_flavonoids_for_the_quality_control_of_Apocynum_venetum_L__leaves_by_HPLC_DAD_ESI_IT_TOF_MS_and_HPLC_DAD_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(13)00301-4 DB - PRIME DP - Unbound Medicine ER -