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Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms.
Biosens Bioelectron. 2014 Jan 15; 51:274-9.BB

Abstract

A whole-cell biosensor assay for dual ZnO nanoparticle toxicity mechanisms has been developed based on the transcriptional response of Escherichia coli to: (1) Zn(2+) from ZnO nanoparticle dissolution with genes zntA (Zn(2+) efflux) and znuABC (Zn(2+) uptake); and (2) redox stress from ZnO nanoparticle photo-electron production under ultraviolet light with genes soxS and katG. Both processes occur in a dispersion of ZnO nanoparticles leading to toxicity. ZnO nanoparticle dissolution was measured independently by ICP-MS and photo-radical generation was confirmed by the stochiometric reduction of the redox dye, 2, 6-dichloroindolphenol (DCPIP). The whole-cell biosensor can detect both toxicity mechanisms and is a species-specific assay capable of discriminating between ZnO nanoparticles and the Zn(2+) dissolution product.

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Authors+Show Affiliations

Mcquillan JS
Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK.
Shaw AM
No affiliation info available

MeSH

Biosensing TechniquesEscherichia coliGene Expression Regulation, BacterialNanoparticlesToxicity TestsZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23974158

Citation

Mcquillan, Jonathan S., and Andrew M. Shaw. "Whole-cell Escherichia Coli-based Bio-sensor Assay for Dual Zinc Oxide Nanoparticle Toxicity Mechanisms." Biosensors & Bioelectronics, vol. 51, 2014, pp. 274-9.
Mcquillan JS, Shaw AM. Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms. Biosens Bioelectron. 2014;51:274-9.
Mcquillan, J. S., & Shaw, A. M. (2014). Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms. Biosensors & Bioelectronics, 51, 274-9. https://doi.org/10.1016/j.bios.2013.07.024
Mcquillan JS, Shaw AM. Whole-cell Escherichia Coli-based Bio-sensor Assay for Dual Zinc Oxide Nanoparticle Toxicity Mechanisms. Biosens Bioelectron. 2014 Jan 15;51:274-9. PubMed PMID: 23974158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms. AU - Mcquillan,Jonathan S, AU - Shaw,Andrew M, Y1 - 2013/07/22/ PY - 2013/04/08/received PY - 2013/06/13/revised PY - 2013/07/12/accepted PY - 2013/8/27/entrez PY - 2013/8/27/pubmed PY - 2014/5/13/medline KW - Escherichia coli KW - Real time PCR KW - Stress response KW - Whole cell biosensor KW - Zinc oxide nanoparticle SP - 274 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 51 N2 - A whole-cell biosensor assay for dual ZnO nanoparticle toxicity mechanisms has been developed based on the transcriptional response of Escherichia coli to: (1) Zn(2+) from ZnO nanoparticle dissolution with genes zntA (Zn(2+) efflux) and znuABC (Zn(2+) uptake); and (2) redox stress from ZnO nanoparticle photo-electron production under ultraviolet light with genes soxS and katG. Both processes occur in a dispersion of ZnO nanoparticles leading to toxicity. ZnO nanoparticle dissolution was measured independently by ICP-MS and photo-radical generation was confirmed by the stochiometric reduction of the redox dye, 2, 6-dichloroindolphenol (DCPIP). The whole-cell biosensor can detect both toxicity mechanisms and is a species-specific assay capable of discriminating between ZnO nanoparticles and the Zn(2+) dissolution product. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23974158/Whole_cell_Escherichia_coli_based_bio_sensor_assay_for_dual_zinc_oxide_nanoparticle_toxicity_mechanisms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00496-X DB - PRIME DP - Unbound Medicine ER -