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Production of indole alkaloids by metabolic engineering of the tryptophan pathway in rice.
Plant Biotechnol J. 2013 Dec; 11(9):1103-11.PB

Abstract

Tryptophan decarboxylase (TDC) converts tryptophan (Trp) into tryptamine, consequently increasing the metabolic flow of tryptophan derivatives into the production of secondary metabolites such as indole alkaloids. We inserted an expression cassette containing OsTDC, a putative tryptophan decarboxylase gene from rice, into an expression plasmid vector containing OASA1D, the feedback-resistant anthranilate synthase alpha-subunit mutant (OASA1D). Overexpression of OASA1D has been reported to significantly increase Trp levels in rice. The co-expression of OsTDC and OASA1D in rice calli led to almost complete depletion of the Trp pool and a consequent increase in the tryptamine pool. This indicates that TDC inactivity is a contributory factor for the accumulation of Trp in rice transgenics overexpressing OASA1D. Metabolic profiling of the calli expressing OsTDC and OASA1D revealed the accumulation of serotonin and serotonin-derived indole compounds (potentially pharmacoactive β-carbolines) that have not been reported from rice. Rice calli overexpressing OASA1D:OASA1D is a novel system for the production of significant amounts of pharmacologically useful indole alkaloids in rice.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dubouzet JG
JST/CREST Plant Functions and Their Control, Japan Science and Technology Agency, Tokyo, Japan; Biotransformation Team, Scion Research, Rotorua, New Zealand.
Matsuda F
No affiliation info available
Ishihara A
No affiliation info available
Miyagawa H
No affiliation info available
Wakasa K
No affiliation info available

MeSH

Anthranilate SynthaseAromatic-L-Amino-Acid DecarboxylasesGene Expression Regulation, EnzymologicGene Expression Regulation, PlantIndole AlkaloidsMetabolic EngineeringMetabolic Networks and PathwaysMetabolomeOryzaPhenotypePhylogenyPlant ProteinsPlants, Genetically ModifiedSeedlingsSerotoninTryptaminesTryptophan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23980801

Citation

Dubouzet, Joseph G., et al. "Production of Indole Alkaloids By Metabolic Engineering of the Tryptophan Pathway in Rice." Plant Biotechnology Journal, vol. 11, no. 9, 2013, pp. 1103-11.
Dubouzet JG, Matsuda F, Ishihara A, et al. Production of indole alkaloids by metabolic engineering of the tryptophan pathway in rice. Plant Biotechnol J. 2013;11(9):1103-11.
Dubouzet, J. G., Matsuda, F., Ishihara, A., Miyagawa, H., & Wakasa, K. (2013). Production of indole alkaloids by metabolic engineering of the tryptophan pathway in rice. Plant Biotechnology Journal, 11(9), 1103-11. https://doi.org/10.1111/pbi.12105
Dubouzet JG, et al. Production of Indole Alkaloids By Metabolic Engineering of the Tryptophan Pathway in Rice. Plant Biotechnol J. 2013;11(9):1103-11. PubMed PMID: 23980801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of indole alkaloids by metabolic engineering of the tryptophan pathway in rice. AU - Dubouzet,Joseph G, AU - Matsuda,Fumio, AU - Ishihara,Atsushi, AU - Miyagawa,Hisashi, AU - Wakasa,Kyo, Y1 - 2013/08/27/ PY - 2013/05/18/received PY - 2013/06/23/revised PY - 2013/07/09/accepted PY - 2013/8/29/entrez PY - 2013/8/29/pubmed PY - 2014/7/22/medline KW - anthranilate synthase KW - serotonin KW - tryptamine KW - tryptophan decarboxylase KW - β-carboline SP - 1103 EP - 11 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 11 IS - 9 N2 - Tryptophan decarboxylase (TDC) converts tryptophan (Trp) into tryptamine, consequently increasing the metabolic flow of tryptophan derivatives into the production of secondary metabolites such as indole alkaloids. We inserted an expression cassette containing OsTDC, a putative tryptophan decarboxylase gene from rice, into an expression plasmid vector containing OASA1D, the feedback-resistant anthranilate synthase alpha-subunit mutant (OASA1D). Overexpression of OASA1D has been reported to significantly increase Trp levels in rice. The co-expression of OsTDC and OASA1D in rice calli led to almost complete depletion of the Trp pool and a consequent increase in the tryptamine pool. This indicates that TDC inactivity is a contributory factor for the accumulation of Trp in rice transgenics overexpressing OASA1D. Metabolic profiling of the calli expressing OsTDC and OASA1D revealed the accumulation of serotonin and serotonin-derived indole compounds (potentially pharmacoactive β-carbolines) that have not been reported from rice. Rice calli overexpressing OASA1D:OASA1D is a novel system for the production of significant amounts of pharmacologically useful indole alkaloids in rice. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/23980801/Production_of_indole_alkaloids_by_metabolic_engineering_of_the_tryptophan_pathway_in_rice_ L2 - https://doi.org/10.1111/pbi.12105 DB - PRIME DP - Unbound Medicine ER -