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Identification of an alternative knockdown resistance (kdr)-like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage-gated sodium channel gene.
Pest Manag Sci. 2014 Jun; 70(6):977-81.PM

Abstract

BACKGROUND

Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid-resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations.

RESULTS

Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda-cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage-gated sodium channel gene. One mutation resulted in the non-synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non-synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda-cyhalothrin resistance.

CONCLUSION

We have characterized a known kdr mutation and identified a novel mutation in the voltage-gated sodium channel gene of Thrips tabaci associated with resistance to lambda-cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wu M
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, P.R. China; Department of Entomology, Washington State University, Pullman, WA, USA.
Gotoh H
No affiliation info available
Waters T
No affiliation info available
Walsh DB
No affiliation info available
Lavine LC
No affiliation info available

MeSH

Gene Knockdown TechniquesInsecticide ResistanceMutationNitrilesPyrethrinsSodium ChannelsThysanopteraWashington

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23983138

Citation

Wu, Meixiang, et al. "Identification of an Alternative Knockdown Resistance (kdr)-like Mutation, M918L, and a Novel Mutation, V1010A, in the Thrips Tabaci Voltage-gated Sodium Channel Gene." Pest Management Science, vol. 70, no. 6, 2014, pp. 977-81.
Wu M, Gotoh H, Waters T, et al. Identification of an alternative knockdown resistance (kdr)-like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage-gated sodium channel gene. Pest Manag Sci. 2014;70(6):977-81.
Wu, M., Gotoh, H., Waters, T., Walsh, D. B., & Lavine, L. C. (2014). Identification of an alternative knockdown resistance (kdr)-like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage-gated sodium channel gene. Pest Management Science, 70(6), 977-81. https://doi.org/10.1002/ps.3638
Wu M, et al. Identification of an Alternative Knockdown Resistance (kdr)-like Mutation, M918L, and a Novel Mutation, V1010A, in the Thrips Tabaci Voltage-gated Sodium Channel Gene. Pest Manag Sci. 2014;70(6):977-81. PubMed PMID: 23983138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of an alternative knockdown resistance (kdr)-like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage-gated sodium channel gene. AU - Wu,Meixiang, AU - Gotoh,Hiroki, AU - Waters,Timothy, AU - Walsh,Douglas B, AU - Lavine,Laura Corley, Y1 - 2013/09/30/ PY - 2013/04/26/received PY - 2013/08/17/revised PY - 2013/08/23/accepted PY - 2013/8/29/entrez PY - 2013/8/29/pubmed PY - 2015/1/6/medline KW - Thrips tabaci KW - kdr KW - point mutation KW - pyrethroid resistance KW - sodium channel SP - 977 EP - 81 JF - Pest management science JO - Pest Manag Sci VL - 70 IS - 6 N2 - BACKGROUND: Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid-resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations. RESULTS: Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda-cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage-gated sodium channel gene. One mutation resulted in the non-synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non-synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda-cyhalothrin resistance. CONCLUSION: We have characterized a known kdr mutation and identified a novel mutation in the voltage-gated sodium channel gene of Thrips tabaci associated with resistance to lambda-cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/23983138/Identification_of_an_alternative_knockdown_resistance__kdr__like_mutation_M918L_and_a_novel_mutation_V1010A_in_the_Thrips_tabaci_voltage_gated_sodium_channel_gene_ DB - PRIME DP - Unbound Medicine ER -
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