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Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women: a comparative analysis of their cytotoxic effects with commercial vaginal probiotics.
Iran Red Crescent Med J. 2013 Mar; 15(3):199-206.IR

Abstract

BACKGROUND

Vaginal flora of healthy women is dominated by Lactobacillus species which can prevent bacterial vaginosis.

OBJECTIVES

The current study aimed to determine the differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis (BV) infected women and compared their cytotoxic effects with commercial vaginal probiotics.

PATIENTS AND METHODS

One hundred and seventy eight vaginal specimens were collected from healthy and BV infected women. Lactobacillus colonies were obtained by culturing on laked blood BHI and MRS medias and genetically defined by 16s rRNA sequencing. Differentiating the specimens to normal, intermediate and BV infected were carried out by Ison and Hey grading protocol. Identification of Lactobacillus strains in vaginal specimens were performed by Multiplex PCR. The inhibitory effects of lactobacilli on Hela (tumoral cervical cells) and HNCF-pi52 (normal cervical cells) were conducted by MTT and trypan blue assays.

RESULTS

L. crispatus, L. gasseri, L. iners, L. jensenii, L. acidophilus and L. rhamnosus were the most frequently occurring species in vagina of healthy Iranian women. L. crispatus and L. jensenni were significantly higher in the normal than in the BV infected groups. Also the cytotoxic effect of L. crispatus on tumoral cervical cells was higher than other lactobacilli including commercial probiotics.

CONCLUSIONS

As L. crispatus and L. jensenni were significantly higher in BV infected women and the cytotoxic effect of L. crispatus on tumoral cervical cells was high, introduction of new probiotics seems necessary.

Authors+Show Affiliations

Medical Genetics Department, Tehran University of Medical Sciences, Tehran, IR Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23983998

Citation

Motevaseli, Elahe, et al. "Differences in Vaginal Lactobacilli Composition of Iranian Healthy and Bacterial Vaginosis Infected Women: a Comparative Analysis of Their Cytotoxic Effects With Commercial Vaginal Probiotics." Iranian Red Crescent Medical Journal, vol. 15, no. 3, 2013, pp. 199-206.
Motevaseli E, Shirzad M, Raoofian R, et al. Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women: a comparative analysis of their cytotoxic effects with commercial vaginal probiotics. Iran Red Crescent Med J. 2013;15(3):199-206.
Motevaseli, E., Shirzad, M., Raoofian, R., Hasheminasab, S. M., Hatami, M., Dianatpour, M., & Modarressi, M. H. (2013). Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women: a comparative analysis of their cytotoxic effects with commercial vaginal probiotics. Iranian Red Crescent Medical Journal, 15(3), 199-206. https://doi.org/10.5812/ircmj.3533
Motevaseli E, et al. Differences in Vaginal Lactobacilli Composition of Iranian Healthy and Bacterial Vaginosis Infected Women: a Comparative Analysis of Their Cytotoxic Effects With Commercial Vaginal Probiotics. Iran Red Crescent Med J. 2013;15(3):199-206. PubMed PMID: 23983998.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis infected women: a comparative analysis of their cytotoxic effects with commercial vaginal probiotics. AU - Motevaseli,Elahe, AU - Shirzad,Mahdieh, AU - Raoofian,Reza, AU - Hasheminasab,Seyyed-Mohammad, AU - Hatami,Maryam, AU - Dianatpour,Mehdi, AU - Modarressi,Mohammad-Hossein, Y1 - 2013/03/05/ PY - 2011/11/14/received PY - 2011/12/26/revised PY - 2012/01/04/accepted PY - 2013/8/29/entrez PY - 2013/8/29/pubmed PY - 2013/8/29/medline KW - Bacterial Vaginosis KW - Iran KW - Probiotic KW - T-Lymphocytes, Cytotoxic KW - Vagina KW - Women SP - 199 EP - 206 JF - Iranian Red Crescent medical journal JO - Iran Red Crescent Med J VL - 15 IS - 3 N2 - BACKGROUND: Vaginal flora of healthy women is dominated by Lactobacillus species which can prevent bacterial vaginosis. OBJECTIVES: The current study aimed to determine the differences in vaginal lactobacilli composition of Iranian healthy and bacterial vaginosis (BV) infected women and compared their cytotoxic effects with commercial vaginal probiotics. PATIENTS AND METHODS: One hundred and seventy eight vaginal specimens were collected from healthy and BV infected women. Lactobacillus colonies were obtained by culturing on laked blood BHI and MRS medias and genetically defined by 16s rRNA sequencing. Differentiating the specimens to normal, intermediate and BV infected were carried out by Ison and Hey grading protocol. Identification of Lactobacillus strains in vaginal specimens were performed by Multiplex PCR. The inhibitory effects of lactobacilli on Hela (tumoral cervical cells) and HNCF-pi52 (normal cervical cells) were conducted by MTT and trypan blue assays. RESULTS: L. crispatus, L. gasseri, L. iners, L. jensenii, L. acidophilus and L. rhamnosus were the most frequently occurring species in vagina of healthy Iranian women. L. crispatus and L. jensenni were significantly higher in the normal than in the BV infected groups. Also the cytotoxic effect of L. crispatus on tumoral cervical cells was higher than other lactobacilli including commercial probiotics. CONCLUSIONS: As L. crispatus and L. jensenni were significantly higher in BV infected women and the cytotoxic effect of L. crispatus on tumoral cervical cells was high, introduction of new probiotics seems necessary. SN - 2074-1804 UR - https://www.unboundmedicine.com/medline/citation/23983998/Differences_in_vaginal_lactobacilli_composition_of_Iranian_healthy_and_bacterial_vaginosis_infected_women:_a_comparative_analysis_of_their_cytotoxic_effects_with_commercial_vaginal_probiotics_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23983998/ DB - PRIME DP - Unbound Medicine ER -