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Protective effects of farrerol against hydrogen-peroxide-induced apoptosis in human endothelium-derived EA.hy926 cells.
Can J Physiol Pharmacol. 2013 Sep; 91(9):733-40.CJ

Abstract

Vascular endothelium plays an important role in the physiological homeostasis of blood vessels. Endothelial injury is considered to be implicated in the pathogenesis of many cardiovascular diseases, including atherosclerosis. Farrerol, a flavonoid considered to be the major bioactive component in a traditional Chinese herb, "Man-shan-hong", which is the dried leaves of Rhododendron dauricum L., displays many bioactive properties, including antibechic, antibacterial, anti-inflammatory, and the inhibition of vascular smooth muscle cell (VSMC) proliferation. In this study, the protective effects of farrerol on hydrogen peroxide (H2O2)-induced apoptosis in human endothelium-derived EA.hy926 cells were investigated. The results showed that farrerol significantly inhibited the loss of cell viability and enhanced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in H2O2-induced EA.hy926 cells. Meanwhile, farrerol inhibited H2O2-induced elevation in the levels of intracellular malondialdehyde and reactive oxygen species, as well as cell apoptosis. Furthermore, real time RT-PCR and Western blot analysis showed that farrerol significantly decreased the expression of Bax mRNA, Bax, cleaved caspase-3, and phosph-p38 MAPK, while increasing the exporession of Bcl-2 mRNA and Bcl-2 in H2O2-induced EA.hy926 cells. These results are the first demonstration that farrerol has protective effects against H2O2-induced apoptosis in EA.hy926 cells, and suggests that farrerol is a potential candidate for the intervention of endothelial-injury-associated cardiovascular diseases.

Authors+Show Affiliations

School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23984825

Citation

Li, Jian-Kuan, et al. "Protective Effects of Farrerol Against Hydrogen-peroxide-induced Apoptosis in Human Endothelium-derived EA.hy926 Cells." Canadian Journal of Physiology and Pharmacology, vol. 91, no. 9, 2013, pp. 733-40.
Li JK, Ge R, Tang L, et al. Protective effects of farrerol against hydrogen-peroxide-induced apoptosis in human endothelium-derived EA.hy926 cells. Can J Physiol Pharmacol. 2013;91(9):733-40.
Li, J. K., Ge, R., Tang, L., & Li, Q. S. (2013). Protective effects of farrerol against hydrogen-peroxide-induced apoptosis in human endothelium-derived EA.hy926 cells. Canadian Journal of Physiology and Pharmacology, 91(9), 733-40. https://doi.org/10.1139/cjpp-2013-0008
Li JK, et al. Protective Effects of Farrerol Against Hydrogen-peroxide-induced Apoptosis in Human Endothelium-derived EA.hy926 Cells. Can J Physiol Pharmacol. 2013;91(9):733-40. PubMed PMID: 23984825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of farrerol against hydrogen-peroxide-induced apoptosis in human endothelium-derived EA.hy926 cells. AU - Li,Jian-Kuan, AU - Ge,Rui, AU - Tang,Li, AU - Li,Qing-Shan, Y1 - 2013/04/22/ PY - 2013/8/30/entrez PY - 2013/8/30/pubmed PY - 2014/3/22/medline SP - 733 EP - 40 JF - Canadian journal of physiology and pharmacology JO - Can. J. Physiol. Pharmacol. VL - 91 IS - 9 N2 - Vascular endothelium plays an important role in the physiological homeostasis of blood vessels. Endothelial injury is considered to be implicated in the pathogenesis of many cardiovascular diseases, including atherosclerosis. Farrerol, a flavonoid considered to be the major bioactive component in a traditional Chinese herb, "Man-shan-hong", which is the dried leaves of Rhododendron dauricum L., displays many bioactive properties, including antibechic, antibacterial, anti-inflammatory, and the inhibition of vascular smooth muscle cell (VSMC) proliferation. In this study, the protective effects of farrerol on hydrogen peroxide (H2O2)-induced apoptosis in human endothelium-derived EA.hy926 cells were investigated. The results showed that farrerol significantly inhibited the loss of cell viability and enhanced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in H2O2-induced EA.hy926 cells. Meanwhile, farrerol inhibited H2O2-induced elevation in the levels of intracellular malondialdehyde and reactive oxygen species, as well as cell apoptosis. Furthermore, real time RT-PCR and Western blot analysis showed that farrerol significantly decreased the expression of Bax mRNA, Bax, cleaved caspase-3, and phosph-p38 MAPK, while increasing the exporession of Bcl-2 mRNA and Bcl-2 in H2O2-induced EA.hy926 cells. These results are the first demonstration that farrerol has protective effects against H2O2-induced apoptosis in EA.hy926 cells, and suggests that farrerol is a potential candidate for the intervention of endothelial-injury-associated cardiovascular diseases. SN - 1205-7541 UR - https://www.unboundmedicine.com/medline/citation/23984825/Protective_effects_of_farrerol_against_hydrogen_peroxide_induced_apoptosis_in_human_endothelium_derived_EA_hy926_cells_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/cjpp-2013-0008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -