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Polysaccharide structure of tetrasporic red seaweed Tichocarpus crinitus.
Carbohydr Polym. 2013 Oct 15; 98(1):26-35.CP

Abstract

Sulfated polysaccharide isolated from tetrasporic plants of Tichocarpus crinitus was investigated. The polysaccharide was isolated by two methods: with water extraction at 80 °C (HT) and with a mild alkaline extraction (AE). The extracted polysaccharides were presented by non-gelling ones only, while galactose and 3,6-AG were the main monosaccharides, at the same time amount of 3,6-AG in AE polysaccharides was the similar to that of HT. According to methods of spectroscopy and mass spectrometry, the polysaccharide from tetrasporic T. crinitus contains main blocks of 1,3-linked β-D-galactopyranosyl-2,4-disulfates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl while 6-sulfated 4-linked galactopyranosyl resudies are randomly distributed along the polysaccharide chain. The alkaline treatment of HT polysaccharide results in obtaining polysaccharide with regular structure that composed of alternating 1,3-linked β-D-galactopyranosyl-2,4-disulfates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl residues. Native polysaccharide (HT) possessed both high anticoagulant and antiplatelet activity measured by fibrin clotting and platelet aggregation induced by collagen. This activity could be connected with peculiar chemical structure of HT polysaccharide which has high sulfation degree and contains also 3,6-anhydrogalactose in the polymer chain.

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Authors+Show Affiliations

Byankina Barabanova AO
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, 159, Vladivostok 690022, Russia.
Sokolova EV
No affiliation info available
Anastyuk SD
No affiliation info available
Isakov VV
No affiliation info available
Glazunov VP
No affiliation info available
Volod'ko AV
No affiliation info available
Yakovleva IM
No affiliation info available
Solov'eva TF
No affiliation info available
Yermak IM
No affiliation info available

MeSH

HumansMolecular WeightPlatelet AggregationPolysaccharidesRhodophytaSeaweedThrombin Time

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23987313

Citation

Byankina Barabanova, A O., et al. "Polysaccharide Structure of Tetrasporic Red Seaweed Tichocarpus Crinitus." Carbohydrate Polymers, vol. 98, no. 1, 2013, pp. 26-35.
Byankina Barabanova AO, Sokolova EV, Anastyuk SD, et al. Polysaccharide structure of tetrasporic red seaweed Tichocarpus crinitus. Carbohydr Polym. 2013;98(1):26-35.
Byankina Barabanova, A. O., Sokolova, E. V., Anastyuk, S. D., Isakov, V. V., Glazunov, V. P., Volod'ko, A. V., Yakovleva, I. M., Solov'eva, T. F., & Yermak, I. M. (2013). Polysaccharide structure of tetrasporic red seaweed Tichocarpus crinitus. Carbohydrate Polymers, 98(1), 26-35. https://doi.org/10.1016/j.carbpol.2013.04.063
Byankina Barabanova AO, et al. Polysaccharide Structure of Tetrasporic Red Seaweed Tichocarpus Crinitus. Carbohydr Polym. 2013 Oct 15;98(1):26-35. PubMed PMID: 23987313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polysaccharide structure of tetrasporic red seaweed Tichocarpus crinitus. AU - Byankina Barabanova,A O, AU - Sokolova,E V, AU - Anastyuk,S D, AU - Isakov,V V, AU - Glazunov,V P, AU - Volod'ko,A V, AU - Yakovleva,I M, AU - Solov'eva,T F, AU - Yermak,I M, Y1 - 2013/04/30/ PY - 2013/02/13/received PY - 2013/04/15/revised PY - 2013/04/16/accepted PY - 2013/8/31/entrez PY - 2013/8/31/pubmed PY - 2014/3/29/medline KW - Carrageenan KW - Life history stage of alga KW - Structure KW - Tetrasporophytes SP - 26 EP - 35 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 98 IS - 1 N2 - Sulfated polysaccharide isolated from tetrasporic plants of Tichocarpus crinitus was investigated. The polysaccharide was isolated by two methods: with water extraction at 80 °C (HT) and with a mild alkaline extraction (AE). The extracted polysaccharides were presented by non-gelling ones only, while galactose and 3,6-AG were the main monosaccharides, at the same time amount of 3,6-AG in AE polysaccharides was the similar to that of HT. According to methods of spectroscopy and mass spectrometry, the polysaccharide from tetrasporic T. crinitus contains main blocks of 1,3-linked β-D-galactopyranosyl-2,4-disulfates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl while 6-sulfated 4-linked galactopyranosyl resudies are randomly distributed along the polysaccharide chain. The alkaline treatment of HT polysaccharide results in obtaining polysaccharide with regular structure that composed of alternating 1,3-linked β-D-galactopyranosyl-2,4-disulfates and 1,4-linked 3,6-anhydro-α-D-galactopyranosyl residues. Native polysaccharide (HT) possessed both high anticoagulant and antiplatelet activity measured by fibrin clotting and platelet aggregation induced by collagen. This activity could be connected with peculiar chemical structure of HT polysaccharide which has high sulfation degree and contains also 3,6-anhydrogalactose in the polymer chain. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/23987313/Polysaccharide_structure_of_tetrasporic_red_seaweed_Tichocarpus_crinitus_ DB - PRIME DP - Unbound Medicine ER -