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Croton antisyphiliticus Mart. attenuates the inflammatory response to carrageenan-induced pleurisy in mice.
Inflammopharmacology. 2014 Apr; 22(2):115-26.I

Abstract

The aim of this study was to investigate the anti-inflammatory effect of the crude hydroalcoholic extract (CHE) from the aerial parts of Croton antisyphiliticus, its fractions and isolated compounds derived from it on the mouse model of pleurisy induced by carrageenan. The aerial parts of C. antisyphiliticus were dried, macerated and extracted with ethanol to obtain the CHE, which was fractionated by liquid-liquid extraction using solvents with increasing polarity to obtain hexane (Hex), ethyl acetate (EA) and aqueous (Aq) fractions. Vitexin and quinic acid were isolated from Aq fraction. Capillary electrophoresis analysis, physical characteristics and spectral data produced by infrared (IR), nuclear magnetic resonance ((1)H and (13)C NMR) and mass spectrometry analyses were used to identify and elucidate the structure of the isolated compounds. The experimental model of pleurisy was induced in mice by a single intrapleural injection of carrageenan (1 %). Leukocytes, exudate concentrations, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities and nitrate/nitrite (NOx), tumor necrosis factor-α (TNF-α) and interleukin-17 (IL-17) levels were determined in the pleural fluid leakage at 4 h after pleurisy induction. Animals pre-treated with CHE, Hex, EA, Aq, vitexin and quinic acid exhibited decreases in leukocytes, exudate concentrations, MPO and ADA activities and NOx levels (p < 0.05). Also CHE, Hex, EA and vitexin but not quinic acid inhibited TNF-α and IL-17 levels (p < 0.05). C. antisyphiliticus caused anti-inflammatory effect by inhibiting the activated leukocytes, exudate concentrations, NOx, TNF-α, and IL-17 levels. The compounds vitexin and quinic acid may be responsible for this anti-inflammatory action.

Authors+Show Affiliations

Department of Clinical Analysis, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário-Trindade, Florianópolis, Santa Catarina, 88040-970, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23990384

Citation

Dos Reis, Gustavo Oliveira, et al. "Croton Antisyphiliticus Mart. Attenuates the Inflammatory Response to Carrageenan-induced Pleurisy in Mice." Inflammopharmacology, vol. 22, no. 2, 2014, pp. 115-26.
Dos Reis GO, Vicente G, de Carvalho FK, et al. Croton antisyphiliticus Mart. attenuates the inflammatory response to carrageenan-induced pleurisy in mice. Inflammopharmacology. 2014;22(2):115-26.
Dos Reis, G. O., Vicente, G., de Carvalho, F. K., Heller, M., Micke, G. A., Pizzolatti, M. G., & Fröde, T. S. (2014). Croton antisyphiliticus Mart. attenuates the inflammatory response to carrageenan-induced pleurisy in mice. Inflammopharmacology, 22(2), 115-26. https://doi.org/10.1007/s10787-013-0184-6
Dos Reis GO, et al. Croton Antisyphiliticus Mart. Attenuates the Inflammatory Response to Carrageenan-induced Pleurisy in Mice. Inflammopharmacology. 2014;22(2):115-26. PubMed PMID: 23990384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Croton antisyphiliticus Mart. attenuates the inflammatory response to carrageenan-induced pleurisy in mice. AU - Dos Reis,Gustavo Oliveira, AU - Vicente,Geison, AU - de Carvalho,Francieli Kanumfre, AU - Heller,Melina, AU - Micke,Gustavo Amadeu, AU - Pizzolatti,Moacir Geraldo, AU - Fröde,Tânia Silvia, Y1 - 2013/08/29/ PY - 2013/06/17/received PY - 2013/07/29/accepted PY - 2013/8/31/entrez PY - 2013/8/31/pubmed PY - 2014/10/30/medline SP - 115 EP - 26 JF - Inflammopharmacology JO - Inflammopharmacology VL - 22 IS - 2 N2 - The aim of this study was to investigate the anti-inflammatory effect of the crude hydroalcoholic extract (CHE) from the aerial parts of Croton antisyphiliticus, its fractions and isolated compounds derived from it on the mouse model of pleurisy induced by carrageenan. The aerial parts of C. antisyphiliticus were dried, macerated and extracted with ethanol to obtain the CHE, which was fractionated by liquid-liquid extraction using solvents with increasing polarity to obtain hexane (Hex), ethyl acetate (EA) and aqueous (Aq) fractions. Vitexin and quinic acid were isolated from Aq fraction. Capillary electrophoresis analysis, physical characteristics and spectral data produced by infrared (IR), nuclear magnetic resonance ((1)H and (13)C NMR) and mass spectrometry analyses were used to identify and elucidate the structure of the isolated compounds. The experimental model of pleurisy was induced in mice by a single intrapleural injection of carrageenan (1 %). Leukocytes, exudate concentrations, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities and nitrate/nitrite (NOx), tumor necrosis factor-α (TNF-α) and interleukin-17 (IL-17) levels were determined in the pleural fluid leakage at 4 h after pleurisy induction. Animals pre-treated with CHE, Hex, EA, Aq, vitexin and quinic acid exhibited decreases in leukocytes, exudate concentrations, MPO and ADA activities and NOx levels (p < 0.05). Also CHE, Hex, EA and vitexin but not quinic acid inhibited TNF-α and IL-17 levels (p < 0.05). C. antisyphiliticus caused anti-inflammatory effect by inhibiting the activated leukocytes, exudate concentrations, NOx, TNF-α, and IL-17 levels. The compounds vitexin and quinic acid may be responsible for this anti-inflammatory action. SN - 1568-5608 UR - https://www.unboundmedicine.com/medline/citation/23990384/Croton_antisyphiliticus_Mart__attenuates_the_inflammatory_response_to_carrageenan_induced_pleurisy_in_mice_ DB - PRIME DP - Unbound Medicine ER -