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Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH).
J Appl Genet. 2013 Nov; 54(4):427-33.JA

Abstract

The aim of the present work was to study chromosomal polymorphism within cultivated barley (Hordeum vulgare ssp. vulgare) using three-color fluorescence in situ hybridization (FISH). The physical distribution of the most frequently used, highly repetitive DNA sequences (GAA)7 specific for pericentromeric heterochromatic regions, the ribosomal DNA clone pTa71, specific for the 45S rDNA, and the barley-specific telomere-associated sequence HvT01, was investigated to reveal genetic diversity in metaphase spreads of ten barley genotypes with diverse geographical origin, growth habit and row number. A wild relative of barley, Hordeum chilense was also studied in order to compare the polymorphism between and within Hordeum species. Significant differences in the hybridization patterns of all three DNA probes could be detected between the two related species, but only probes pTa71 and HvT01 showed variation in the intensity and/or position of hybridization sites among genotypes of H. vulgare ssp. vulgare. The extent of polymorphism was less than that earlier reported for molecular markers and was restricted to the long chromosome arms, with differences between the chromosomes. 1H and 3H proved to be the most variable chromosomes and 4H and 6H the most conserved.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Szakács É
Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462, Martonvásár, POB 19, Hungary, szakacse61@gmail.com.
Kruppa K
No affiliation info available
Molnár-Láng M
No affiliation info available

MeSH

Chromosomes, PlantDNA ProbesDNA, PlantDNA, RibosomalGenotypeHordeumIn Situ Hybridization, FluorescencePolymorphism, GeneticSequence Analysis, DNA

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23990510

Citation

Szakács, É, et al. "Analysis of Chromosomal Polymorphism in Barley (Hordeum Vulgare L. Ssp. Vulgare) and Between H. Vulgare and H. Chilense Using Three-color Fluorescence in Situ Hybridization (FISH)." Journal of Applied Genetics, vol. 54, no. 4, 2013, pp. 427-33.
Szakács É, Kruppa K, Molnár-Láng M. Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH). J Appl Genet. 2013;54(4):427-33.
Szakács, É., Kruppa, K., & Molnár-Láng, M. (2013). Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH). Journal of Applied Genetics, 54(4), 427-33. https://doi.org/10.1007/s13353-013-0167-8
Szakács É, Kruppa K, Molnár-Láng M. Analysis of Chromosomal Polymorphism in Barley (Hordeum Vulgare L. Ssp. Vulgare) and Between H. Vulgare and H. Chilense Using Three-color Fluorescence in Situ Hybridization (FISH). J Appl Genet. 2013;54(4):427-33. PubMed PMID: 23990510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH). AU - Szakács,É, AU - Kruppa,K, AU - Molnár-Láng,M, Y1 - 2013/08/29/ PY - 2013/05/27/received PY - 2013/08/05/accepted PY - 2013/07/31/revised PY - 2013/8/31/entrez PY - 2013/8/31/pubmed PY - 2014/7/10/medline SP - 427 EP - 33 JF - Journal of applied genetics JO - J Appl Genet VL - 54 IS - 4 N2 - The aim of the present work was to study chromosomal polymorphism within cultivated barley (Hordeum vulgare ssp. vulgare) using three-color fluorescence in situ hybridization (FISH). The physical distribution of the most frequently used, highly repetitive DNA sequences (GAA)7 specific for pericentromeric heterochromatic regions, the ribosomal DNA clone pTa71, specific for the 45S rDNA, and the barley-specific telomere-associated sequence HvT01, was investigated to reveal genetic diversity in metaphase spreads of ten barley genotypes with diverse geographical origin, growth habit and row number. A wild relative of barley, Hordeum chilense was also studied in order to compare the polymorphism between and within Hordeum species. Significant differences in the hybridization patterns of all three DNA probes could be detected between the two related species, but only probes pTa71 and HvT01 showed variation in the intensity and/or position of hybridization sites among genotypes of H. vulgare ssp. vulgare. The extent of polymorphism was less than that earlier reported for molecular markers and was restricted to the long chromosome arms, with differences between the chromosomes. 1H and 3H proved to be the most variable chromosomes and 4H and 6H the most conserved. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/23990510/Analysis_of_chromosomal_polymorphism_in_barley__Hordeum_vulgare_L__ssp__vulgare__and_between_H__vulgare_and_H__chilense_using_three_color_fluorescence_in_situ_hybridization__FISH__ DB - PRIME DP - Unbound Medicine ER -