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Electrospun poly(L-lactide)/poly(ε-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells.
PLoS One. 2013; 8(8):e71265.Plos

Abstract

The essence of tissue engineering is the fabrication of autologous cells or induced stem cells in naturally derived or synthetic scaffolds to form specific tissues. Polymer is thought as an appealing source of cell-seeded scaffold owing to the diversity of its physicochemical property and can be electrospun into nano-size to mimic natural structure. Poly (L-lactic acid) (PLLA) and poly (ε-caprolactone) (PCL) are both excellent aliphatic polyester with almost "opposite" characteristics. The controlling combination of PLLA and PCL provides varying properties and makes diverse applications. Compared with the copolymers of the same components, PLLA/PCL blend demonstrates its potential in regenerative medicine as a simple, efficient and scalable alternative. In this study, we electrospun PLLA/PCL blends of different weight ratios into nanofibrous scaffolds (NFS) and their properties were detected including morphology, porosity, degradation, ATR-FTIR analysis, stress-stain assay, and inflammatory reaction. To explore the biocompatibility of the NFS we synthesized, human adipose-derived stem cells (hASCs) were used to evaluate proliferation, attachment, viability and multi-lineage differentiation. In conclusion, the electrospun PLLA/PCL blend nanofibrous scaffold with the indicated weight ratios all supported hASCs well. However, the NFS of 1/1 weight ratio showed better properties and cellular responses in all assessments, implying it a biocompatible scaffold for tissue engineering.

Authors+Show Affiliations

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23990941

Citation

Chen, Liang, et al. "Electrospun poly(L-lactide)/poly(ε-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility With Human Adipose-derived Stem Cells." PloS One, vol. 8, no. 8, 2013, pp. e71265.
Chen L, Bai Y, Liao G, et al. Electrospun poly(L-lactide)/poly(ε-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells. PLoS ONE. 2013;8(8):e71265.
Chen, L., Bai, Y., Liao, G., Peng, E., Wu, B., Wang, Y., Zeng, X., & Xie, X. (2013). Electrospun poly(L-lactide)/poly(ε-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells. PloS One, 8(8), e71265. https://doi.org/10.1371/journal.pone.0071265
Chen L, et al. Electrospun poly(L-lactide)/poly(ε-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility With Human Adipose-derived Stem Cells. PLoS ONE. 2013;8(8):e71265. PubMed PMID: 23990941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrospun poly(L-lactide)/poly(ε-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells. AU - Chen,Liang, AU - Bai,Yi, AU - Liao,Guiying, AU - Peng,Ejun, AU - Wu,Bolin, AU - Wang,Yuxi, AU - Zeng,Xiaoyong, AU - Xie,Xiaolin, Y1 - 2013/08/26/ PY - 2013/03/14/received PY - 2013/06/27/accepted PY - 2013/8/31/entrez PY - 2013/8/31/pubmed PY - 2014/4/23/medline SP - e71265 EP - e71265 JF - PloS one JO - PLoS ONE VL - 8 IS - 8 N2 - The essence of tissue engineering is the fabrication of autologous cells or induced stem cells in naturally derived or synthetic scaffolds to form specific tissues. Polymer is thought as an appealing source of cell-seeded scaffold owing to the diversity of its physicochemical property and can be electrospun into nano-size to mimic natural structure. Poly (L-lactic acid) (PLLA) and poly (ε-caprolactone) (PCL) are both excellent aliphatic polyester with almost "opposite" characteristics. The controlling combination of PLLA and PCL provides varying properties and makes diverse applications. Compared with the copolymers of the same components, PLLA/PCL blend demonstrates its potential in regenerative medicine as a simple, efficient and scalable alternative. In this study, we electrospun PLLA/PCL blends of different weight ratios into nanofibrous scaffolds (NFS) and their properties were detected including morphology, porosity, degradation, ATR-FTIR analysis, stress-stain assay, and inflammatory reaction. To explore the biocompatibility of the NFS we synthesized, human adipose-derived stem cells (hASCs) were used to evaluate proliferation, attachment, viability and multi-lineage differentiation. In conclusion, the electrospun PLLA/PCL blend nanofibrous scaffold with the indicated weight ratios all supported hASCs well. However, the NFS of 1/1 weight ratio showed better properties and cellular responses in all assessments, implying it a biocompatible scaffold for tissue engineering. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23990941/Electrospun_poly_L_lactide_/poly_ε_caprolactone__blend_nanofibrous_scaffold:_characterization_and_biocompatibility_with_human_adipose_derived_stem_cells_ L2 - http://dx.plos.org/10.1371/journal.pone.0071265 DB - PRIME DP - Unbound Medicine ER -