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Porphyromonas gingivalis and Treponema denticola synergistic polymicrobial biofilm development.
PLoS One. 2013; 8(8):e71727.Plos

Abstract

Chronic periodontitis has a polymicrobial biofilm aetiology and interactions between key bacterial species are strongly implicated as contributing to disease progression. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia have all been implicated as playing roles in disease progression. P. gingivalis cell-surface-located protease/adhesins, the gingipains, have been suggested to be involved in its interactions with several other bacterial species. The aims of this study were to determine polymicrobial biofilm formation by P. gingivalis, T. denticola and T. forsythia, as well as the role of P. gingivalis gingipains in biofilm formation by using a gingipain null triple mutant. To determine homotypic and polymicrobial biofilm formation a flow cell system was employed and the biofilms imaged and quantified by fluorescent in situ hybridization using DNA species-specific probes and confocal scanning laser microscopy imaging. Of the three species, only P. gingivalis and T. denticola formed mature, homotypic biofilms, and a strong synergy was observed between P. gingivalis and T. denticola in polymicrobial biofilm formation. This synergy was demonstrated by significant increases in biovolume, average biofilm thickness and maximum biofilm thickness of both species. In addition there was a morphological change of T. denticola in polymicrobial biofilms when compared with homotypic biofilms, suggesting reduced motility in homotypic biofilms. P. gingivalis gingipains were shown to play an essential role in synergistic polymicrobial biofilm formation with T. denticola.

Authors+Show Affiliations

Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23990979

Citation

Zhu, Ying, et al. "Porphyromonas Gingivalis and Treponema Denticola Synergistic Polymicrobial Biofilm Development." PloS One, vol. 8, no. 8, 2013, pp. e71727.
Zhu Y, Dashper SG, Chen YY, et al. Porphyromonas gingivalis and Treponema denticola synergistic polymicrobial biofilm development. PLoS One. 2013;8(8):e71727.
Zhu, Y., Dashper, S. G., Chen, Y. Y., Crawford, S., Slakeski, N., & Reynolds, E. C. (2013). Porphyromonas gingivalis and Treponema denticola synergistic polymicrobial biofilm development. PloS One, 8(8), e71727. https://doi.org/10.1371/journal.pone.0071727
Zhu Y, et al. Porphyromonas Gingivalis and Treponema Denticola Synergistic Polymicrobial Biofilm Development. PLoS One. 2013;8(8):e71727. PubMed PMID: 23990979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Porphyromonas gingivalis and Treponema denticola synergistic polymicrobial biofilm development. AU - Zhu,Ying, AU - Dashper,Stuart G, AU - Chen,Yu-Yen, AU - Crawford,Simon, AU - Slakeski,Nada, AU - Reynolds,Eric C, Y1 - 2013/08/26/ PY - 2013/05/08/received PY - 2013/07/08/accepted PY - 2013/8/31/entrez PY - 2013/8/31/pubmed PY - 2014/4/22/medline SP - e71727 EP - e71727 JF - PloS one JO - PLoS One VL - 8 IS - 8 N2 - Chronic periodontitis has a polymicrobial biofilm aetiology and interactions between key bacterial species are strongly implicated as contributing to disease progression. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia have all been implicated as playing roles in disease progression. P. gingivalis cell-surface-located protease/adhesins, the gingipains, have been suggested to be involved in its interactions with several other bacterial species. The aims of this study were to determine polymicrobial biofilm formation by P. gingivalis, T. denticola and T. forsythia, as well as the role of P. gingivalis gingipains in biofilm formation by using a gingipain null triple mutant. To determine homotypic and polymicrobial biofilm formation a flow cell system was employed and the biofilms imaged and quantified by fluorescent in situ hybridization using DNA species-specific probes and confocal scanning laser microscopy imaging. Of the three species, only P. gingivalis and T. denticola formed mature, homotypic biofilms, and a strong synergy was observed between P. gingivalis and T. denticola in polymicrobial biofilm formation. This synergy was demonstrated by significant increases in biovolume, average biofilm thickness and maximum biofilm thickness of both species. In addition there was a morphological change of T. denticola in polymicrobial biofilms when compared with homotypic biofilms, suggesting reduced motility in homotypic biofilms. P. gingivalis gingipains were shown to play an essential role in synergistic polymicrobial biofilm formation with T. denticola. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23990979/Porphyromonas_gingivalis_and_Treponema_denticola_synergistic_polymicrobial_biofilm_development_ L2 - https://dx.plos.org/10.1371/journal.pone.0071727 DB - PRIME DP - Unbound Medicine ER -