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Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses.
Vet J. 2013 Oct; 198(1):217-23.VJ

Abstract

Platelet-rich plasma (PRP) products may be useful for treatment of joint disease in horses, but may contain undesirable pro-inflammatory cytokines in addition to growth factors. This study investigated whether autologous PRP increases synovial fluid growth factor and cytokine concentrations when injected into normal equine metacarpophalangeal and metatarsophalangeal (fetlock) joints. Fetlock joints of seven healthy horses received one of four treatments: saline, resting PRP, CaCl2-activated PRP or thrombin-activated PRP. Synovial fluid was sampled prior to injection and at 6, 24, 48 and 96 h post-injection. Platelet-derived growth factor (PDGF-BB), transforming growth factor β1 (TGFβ1), interleukin (IL)-6 and tumor necrosis factor α (TNFα) concentrations in synovial fluid and PRP were measured by ELISA. Synovial fluid PDGF-BB, TGFβ1, IL-6, TNFα and IL-1 concentrations were also measured in vitro after incubation for 6h with resting PRP only. Growth factor concentrations, but not cytokine concentrations, were significantly higher in activated PRP than in resting PRP samples. After intra-articular injection with resting or thrombin-activated PRP, synovial TGFβ1 increased significantly compared to baseline levels. TNFα and IL-6 were significantly increased in synovial fluid after thrombin-activated PRP injection. In vitro, growth factor concentrations increased significantly in synovial fluid after mixing with PRP, indicating that exogenous activation of PRP for intra-articular injection may be unnecessary, whereas cytokine levels did not. In conclusion, thrombin-activated PRP induced an inflammatory cytokine response in joints, whereas resting or CaCl2-activated PRP did not. Synovial growth factor levels were low overall; the reported benefits of intra-articular PRP may not be attributable to changes in local PDGF or TGFβ1 concentrations.

Authors+Show Affiliations

Department of Pathology, Microbiology and Immunology, University of California, Davis, CA 95616, USA. Electronic address: jamietextor@gmail.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23992870

Citation

Textor, Jamie A., et al. "Synovial Fluid Growth Factor and Cytokine Concentrations After Intra-articular Injection of a Platelet-rich Product in Horses." Veterinary Journal (London, England : 1997), vol. 198, no. 1, 2013, pp. 217-23.
Textor JA, Willits NH, Tablin F. Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. Vet J. 2013;198(1):217-23.
Textor, J. A., Willits, N. H., & Tablin, F. (2013). Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. Veterinary Journal (London, England : 1997), 198(1), 217-23. https://doi.org/10.1016/j.tvjl.2013.07.020
Textor JA, Willits NH, Tablin F. Synovial Fluid Growth Factor and Cytokine Concentrations After Intra-articular Injection of a Platelet-rich Product in Horses. Vet J. 2013;198(1):217-23. PubMed PMID: 23992870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synovial fluid growth factor and cytokine concentrations after intra-articular injection of a platelet-rich product in horses. AU - Textor,Jamie A, AU - Willits,Neil H, AU - Tablin,Fern, Y1 - 2013/08/28/ PY - 2013/04/04/received PY - 2013/07/05/revised PY - 2013/07/14/accepted PY - 2013/9/3/entrez PY - 2013/9/3/pubmed PY - 2014/6/20/medline KW - Cytokines KW - Equine KW - Growth factors KW - Osteoarthritis KW - Platelet-rich plasma SP - 217 EP - 23 JF - Veterinary journal (London, England : 1997) JO - Vet. J. VL - 198 IS - 1 N2 - Platelet-rich plasma (PRP) products may be useful for treatment of joint disease in horses, but may contain undesirable pro-inflammatory cytokines in addition to growth factors. This study investigated whether autologous PRP increases synovial fluid growth factor and cytokine concentrations when injected into normal equine metacarpophalangeal and metatarsophalangeal (fetlock) joints. Fetlock joints of seven healthy horses received one of four treatments: saline, resting PRP, CaCl2-activated PRP or thrombin-activated PRP. Synovial fluid was sampled prior to injection and at 6, 24, 48 and 96 h post-injection. Platelet-derived growth factor (PDGF-BB), transforming growth factor β1 (TGFβ1), interleukin (IL)-6 and tumor necrosis factor α (TNFα) concentrations in synovial fluid and PRP were measured by ELISA. Synovial fluid PDGF-BB, TGFβ1, IL-6, TNFα and IL-1 concentrations were also measured in vitro after incubation for 6h with resting PRP only. Growth factor concentrations, but not cytokine concentrations, were significantly higher in activated PRP than in resting PRP samples. After intra-articular injection with resting or thrombin-activated PRP, synovial TGFβ1 increased significantly compared to baseline levels. TNFα and IL-6 were significantly increased in synovial fluid after thrombin-activated PRP injection. In vitro, growth factor concentrations increased significantly in synovial fluid after mixing with PRP, indicating that exogenous activation of PRP for intra-articular injection may be unnecessary, whereas cytokine levels did not. In conclusion, thrombin-activated PRP induced an inflammatory cytokine response in joints, whereas resting or CaCl2-activated PRP did not. Synovial growth factor levels were low overall; the reported benefits of intra-articular PRP may not be attributable to changes in local PDGF or TGFβ1 concentrations. SN - 1532-2971 UR - https://www.unboundmedicine.com/medline/citation/23992870/Synovial_fluid_growth_factor_and_cytokine_concentrations_after_intra_articular_injection_of_a_platelet_rich_product_in_horses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1090-0233(13)00343-2 DB - PRIME DP - Unbound Medicine ER -