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Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit.
Food Chem. 2013 Dec 15; 141(4):4295-302.FC

Abstract

Freeze-dried fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit were extracted sequentially using non-polar to polar solvents, with further separation carried out on polar extracts by molecular weight cut off dialysis. The fenugreek ethyl acetate crude extract (FGE3) demonstrated the highest antioxidant activity, in terms of Trolox Equivalents (TE), for both the DPPH (35.338±0.908 mg TE/g) and FRAP (77.352±0.627 mg TE/g) assays. This extract also contained the highest phenolic content, in terms of Gallic Acid Equivalents (GAE) (106.316±0.377 mg GAE/g). Despite having considerably lower antioxidant activity than fenugreek, the highest antioxidant activities for bitter fruit were observed in the hexane (BME1) and methanol hydrophilic<3.5 kDa dialysed (BME4<3.5 kDa) extracts, while the highest phenolic content was found in the methanol hydrophilic>3.5 kDa (BME4>3.5 kDa) dialysed extract. UPLC-MS was used to quantify 18 phenolic compounds from fenugreek and 13 from bitter melon in active crude extracts. The flavonoids apigenin-7-O-glycoside (1955.55 ng/mg) and luteolin-7-O-glycoside (725.50 ng/mg) were the most abundant compounds in FGE3, while bitter melon extracts contained only small amounts of mainly phenolic acids. A further 5 fenugreek and 1 bitter melon compounds were identified in trace amounts from the same extracts, respectively.

Authors+Show Affiliations

Department of Food Biosciences, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23993618

Citation

Kenny, O, et al. "Antioxidant Properties and Quantitative UPLC-MS Analysis of Phenolic Compounds From Extracts of Fenugreek (Trigonella Foenum-graecum) Seeds and Bitter Melon (Momordica Charantia) Fruit." Food Chemistry, vol. 141, no. 4, 2013, pp. 4295-302.
Kenny O, Smyth TJ, Hewage CM, et al. Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chem. 2013;141(4):4295-302.
Kenny, O., Smyth, T. J., Hewage, C. M., & Brunton, N. P. (2013). Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chemistry, 141(4), 4295-302. https://doi.org/10.1016/j.foodchem.2013.07.016
Kenny O, et al. Antioxidant Properties and Quantitative UPLC-MS Analysis of Phenolic Compounds From Extracts of Fenugreek (Trigonella Foenum-graecum) Seeds and Bitter Melon (Momordica Charantia) Fruit. Food Chem. 2013 Dec 15;141(4):4295-302. PubMed PMID: 23993618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. AU - Kenny,O, AU - Smyth,T J, AU - Hewage,C M, AU - Brunton,N P, Y1 - 2013/07/10/ PY - 2013/03/25/received PY - 2013/07/01/revised PY - 2013/07/02/accepted PY - 2013/9/3/entrez PY - 2013/9/3/pubmed PY - 2014/3/29/medline KW - Antioxidant KW - Bitter melon KW - Dialysis KW - Fenugreek KW - Phenolics and flavonoids KW - Sequential extraction KW - UPLC-MS SP - 4295 EP - 302 JF - Food chemistry JO - Food Chem VL - 141 IS - 4 N2 - Freeze-dried fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit were extracted sequentially using non-polar to polar solvents, with further separation carried out on polar extracts by molecular weight cut off dialysis. The fenugreek ethyl acetate crude extract (FGE3) demonstrated the highest antioxidant activity, in terms of Trolox Equivalents (TE), for both the DPPH (35.338±0.908 mg TE/g) and FRAP (77.352±0.627 mg TE/g) assays. This extract also contained the highest phenolic content, in terms of Gallic Acid Equivalents (GAE) (106.316±0.377 mg GAE/g). Despite having considerably lower antioxidant activity than fenugreek, the highest antioxidant activities for bitter fruit were observed in the hexane (BME1) and methanol hydrophilic<3.5 kDa dialysed (BME4<3.5 kDa) extracts, while the highest phenolic content was found in the methanol hydrophilic>3.5 kDa (BME4>3.5 kDa) dialysed extract. UPLC-MS was used to quantify 18 phenolic compounds from fenugreek and 13 from bitter melon in active crude extracts. The flavonoids apigenin-7-O-glycoside (1955.55 ng/mg) and luteolin-7-O-glycoside (725.50 ng/mg) were the most abundant compounds in FGE3, while bitter melon extracts contained only small amounts of mainly phenolic acids. A further 5 fenugreek and 1 bitter melon compounds were identified in trace amounts from the same extracts, respectively. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23993618/Antioxidant_properties_and_quantitative_UPLC_MS_analysis_of_phenolic_compounds_from_extracts_of_fenugreek__Trigonella_foenum_graecum__seeds_and_bitter_melon__Momordica_charantia__fruit_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(13)00937-0 DB - PRIME DP - Unbound Medicine ER -