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Endogenous synthesis of n-3 PUFA modifies fatty acid composition of kidney phospholipids and eicosanoid levels in the fat-1 mouse.

Abstract

The goal of the present study was to determine whether endogenous synthesis of n-3 polyunsaturated fatty acids (PUFA) in the fat-1 mouse is comparable to fish oil feeding with respect to kidney n-3 PUFA composition and eicosanoid levels. Wild-type and heterozygous fat-1 mice, capable of synthesizing n-3 PUFA endogenously, were given diets enriched in either n-3 or n-6 PUFA in a 2×2 factorial design and terminated after 12 weeks. Kidney phospholipid fatty acids were analysed by gas chromatography. Kidney eicosanoids were analysed by liquid chromatography tandem mass spectrometry. Relative to control mice fed n-6 PUFA, n-3 PUFA fed and fat-1 mice had higher levels of kidney phospholipid n-3 PUFA, and lower levels of n-6 PUFA and eicosanoids. However, mice fed n-3 PUFA mice had higher levels of n-3 PUFA and lower levels of eicosanoids as compared to fat-1 mice. In conclusion, diet feeding had a greater impact on kidney fatty acid composition and eicosanoid levels than the genetic effect of the fat-1 gene. However, the fat-1 mouse remains a close approximation that can be used as a complementary model to study the role of n-3 PUFA in the kidney.

Authors+Show Affiliations

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23994161

Citation

Kelton, D, et al. "Endogenous Synthesis of N-3 PUFA Modifies Fatty Acid Composition of Kidney Phospholipids and Eicosanoid Levels in the Fat-1 Mouse." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 89, no. 4, 2013, pp. 169-77.
Kelton D, Lysecki C, Aukema H, et al. Endogenous synthesis of n-3 PUFA modifies fatty acid composition of kidney phospholipids and eicosanoid levels in the fat-1 mouse. Prostaglandins Leukot Essent Fatty Acids. 2013;89(4):169-77.
Kelton, D., Lysecki, C., Aukema, H., Anderson, B., Kang, J. X., & Ma, D. W. (2013). Endogenous synthesis of n-3 PUFA modifies fatty acid composition of kidney phospholipids and eicosanoid levels in the fat-1 mouse. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 89(4), pp. 169-77. doi:10.1016/j.plefa.2013.08.002.
Kelton D, et al. Endogenous Synthesis of N-3 PUFA Modifies Fatty Acid Composition of Kidney Phospholipids and Eicosanoid Levels in the Fat-1 Mouse. Prostaglandins Leukot Essent Fatty Acids. 2013;89(4):169-77. PubMed PMID: 23994161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endogenous synthesis of n-3 PUFA modifies fatty acid composition of kidney phospholipids and eicosanoid levels in the fat-1 mouse. AU - Kelton,D, AU - Lysecki,C, AU - Aukema,H, AU - Anderson,B, AU - Kang,J X, AU - Ma,D W L, Y1 - 2013/08/13/ PY - 2013/03/02/received PY - 2013/07/29/revised PY - 2013/08/02/accepted PY - 2013/9/3/entrez PY - 2013/9/3/pubmed PY - 2014/5/20/medline KW - Arachidonic acid KW - Docosahexaenoic acid KW - Eicosanoids KW - Eicosapentaenoic acid KW - Fat-1 KW - Kidney KW - Phospholipid KW - Polyunsaturated fatty acids SP - 169 EP - 77 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 89 IS - 4 N2 - The goal of the present study was to determine whether endogenous synthesis of n-3 polyunsaturated fatty acids (PUFA) in the fat-1 mouse is comparable to fish oil feeding with respect to kidney n-3 PUFA composition and eicosanoid levels. Wild-type and heterozygous fat-1 mice, capable of synthesizing n-3 PUFA endogenously, were given diets enriched in either n-3 or n-6 PUFA in a 2×2 factorial design and terminated after 12 weeks. Kidney phospholipid fatty acids were analysed by gas chromatography. Kidney eicosanoids were analysed by liquid chromatography tandem mass spectrometry. Relative to control mice fed n-6 PUFA, n-3 PUFA fed and fat-1 mice had higher levels of kidney phospholipid n-3 PUFA, and lower levels of n-6 PUFA and eicosanoids. However, mice fed n-3 PUFA mice had higher levels of n-3 PUFA and lower levels of eicosanoids as compared to fat-1 mice. In conclusion, diet feeding had a greater impact on kidney fatty acid composition and eicosanoid levels than the genetic effect of the fat-1 gene. However, the fat-1 mouse remains a close approximation that can be used as a complementary model to study the role of n-3 PUFA in the kidney. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/23994161/Endogenous_synthesis_of_n_3_PUFA_modifies_fatty_acid_composition_of_kidney_phospholipids_and_eicosanoid_levels_in_the_fat_1_mouse_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(13)00159-2 DB - PRIME DP - Unbound Medicine ER -