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Cytochrome b5 reductase encoded by CBR1 is essential for a functional male gametophyte in Arabidopsis.
Plant Cell. 2013 Aug; 25(8):3052-66.PC

Abstract

In all eukaryotes, NADH:cytochrome b5 reductase provides electrons, via cytochrome b5, for a range of biochemical reactions in cellular metabolism, including for fatty acid desaturation in the endoplasmic reticulum. Studies in mammals, yeast, and in vitro plant systems have shown that cytochrome b5 can, at least in some circumstances, also accept electrons from NADPH:cytochrome P450 reductase, potentially allowing for redundancy in reductase function. Here, we report characterization of three T-DNA insertional mutants of the gene encoding cytochrome b5 reductase in Arabidopsis thaliana, CBR1. The progeny of plants heterozygous for the cbr1-2 allele segregated 6% homozygous mutants, while cbr1-3 and cbr1-4 heterozygotes segregated 1:1 heterozygous:wild type, indicating a gametophyte defect. Homozygous cbr1-2 seeds were deformed and required Suc for successful germination and seedling establishment. Vegetative growth of cbr1-2 plants was relatively normal, and they produced abundant flowers, but very few seeds. The pollen produced in cbr1-2 anthers was viable, but when germinated on cbr1-2 or wild-type stigmas, most of the resulting pollen tubes did not extend into the transmitting tract, resulting in a very low frequency of fertilization. These results indicate that cytochrome b5 reductase is not essential during vegetative growth but is required for correct pollen function and seed maturation.

Authors+Show Affiliations

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

23995085

Citation

Wayne, Laura L., et al. "Cytochrome B5 Reductase Encoded By CBR1 Is Essential for a Functional Male Gametophyte in Arabidopsis." The Plant Cell, vol. 25, no. 8, 2013, pp. 3052-66.
Wayne LL, Wallis JG, Kumar R, et al. Cytochrome b5 reductase encoded by CBR1 is essential for a functional male gametophyte in Arabidopsis. Plant Cell. 2013;25(8):3052-66.
Wayne, L. L., Wallis, J. G., Kumar, R., Markham, J. E., & Browse, J. (2013). Cytochrome b5 reductase encoded by CBR1 is essential for a functional male gametophyte in Arabidopsis. The Plant Cell, 25(8), 3052-66. https://doi.org/10.1105/tpc.113.113324
Wayne LL, et al. Cytochrome B5 Reductase Encoded By CBR1 Is Essential for a Functional Male Gametophyte in Arabidopsis. Plant Cell. 2013;25(8):3052-66. PubMed PMID: 23995085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochrome b5 reductase encoded by CBR1 is essential for a functional male gametophyte in Arabidopsis. AU - Wayne,Laura L, AU - Wallis,James G, AU - Kumar,Rajesh, AU - Markham,Jonathan E, AU - Browse,John, Y1 - 2013/08/30/ PY - 2013/9/3/entrez PY - 2013/9/3/pubmed PY - 2014/4/23/medline SP - 3052 EP - 66 JF - The Plant cell JO - Plant Cell VL - 25 IS - 8 N2 - In all eukaryotes, NADH:cytochrome b5 reductase provides electrons, via cytochrome b5, for a range of biochemical reactions in cellular metabolism, including for fatty acid desaturation in the endoplasmic reticulum. Studies in mammals, yeast, and in vitro plant systems have shown that cytochrome b5 can, at least in some circumstances, also accept electrons from NADPH:cytochrome P450 reductase, potentially allowing for redundancy in reductase function. Here, we report characterization of three T-DNA insertional mutants of the gene encoding cytochrome b5 reductase in Arabidopsis thaliana, CBR1. The progeny of plants heterozygous for the cbr1-2 allele segregated 6% homozygous mutants, while cbr1-3 and cbr1-4 heterozygotes segregated 1:1 heterozygous:wild type, indicating a gametophyte defect. Homozygous cbr1-2 seeds were deformed and required Suc for successful germination and seedling establishment. Vegetative growth of cbr1-2 plants was relatively normal, and they produced abundant flowers, but very few seeds. The pollen produced in cbr1-2 anthers was viable, but when germinated on cbr1-2 or wild-type stigmas, most of the resulting pollen tubes did not extend into the transmitting tract, resulting in a very low frequency of fertilization. These results indicate that cytochrome b5 reductase is not essential during vegetative growth but is required for correct pollen function and seed maturation. SN - 1532-298X UR - https://www.unboundmedicine.com/medline/citation/23995085/Cytochrome_b5_reductase_encoded_by_CBR1_is_essential_for_a_functional_male_gametophyte_in_Arabidopsis_ L2 - https://academic.oup.com/plcell/article-lookup/doi/10.1105/tpc.113.113324 DB - PRIME DP - Unbound Medicine ER -