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Effects of reproduction on immuno-suppression and oxidative damage, and hence support or otherwise for their roles as mechanisms underpinning life history trade-offs, are tissue and assay dependent.
J Exp Biol. 2013 Nov 15; 216(Pt 22):4242-50.JE

Abstract

Life history parameters appear to be traded off against each other, but the physiological mechanisms involved remain unclear. One hypothesis is that potentially energetically costly processes such as immune function and protection from oxidative stress may be compromised during reproductive attempts because of selective resource allocation. Lower temperatures also impose energy costs, and hence allocation decisions might be more pronounced when animals are forced to reproduce in the cold. Here, we experimentally tested whether reproduction at different ambient temperatures was associated with elevated oxidative stress and suppressed immune function in Mongolian gerbils (Meriones unguiculatus). Using a variety of different markers for both immune function and oxidative stress, we found that some measures of immune function (serum bactericidal capacity and size of the thymus) were significantly suppressed, while some measures of oxidative protection [serum superoxide dismutase (SOD) activity and glutathione peroxidase (GPx) activity] were also reduced, and a marker of oxidative damage (protein carbonyls in serum) was increased in lactating compared with non-reproductive gerbils. These changes were in line with the selective resource allocation predictions. However, the phytohaemagglutinin response and serum total immunoglobulin (IgG) were not suppressed, and other markers of oxidative damage [malondialdehyde (MDA) (TBARS) and protein carbonyls in the liver] were actually lower in lactating compared with non-reproductive gerbils, consistent with increased levels of SOD activity and total antioxidant capacity in the liver. These latter changes were opposite of the expectations based on resource allocation. Furthermore, other measures of protection (GPx levels in the liver and protein thiols in both serum and liver) and damage [MDA (TBARS) in serum] were unrelated to reproductive status. Ambient temperature differences did not impact on these patterns. Collectively, our results indicated that the inferred effects of reproduction on immunosuppression and oxidative damage, and hence support or otherwise for particular physiological mechanisms that underpin life history trade-offs, are critically dependent on the exact markers and tissues used. This may be because during reproduction individuals selectively allocate protection to some key tissues, but sacrifice protection of others.

Authors+Show Affiliations

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23997195

Citation

Yang, Deng-Bao, et al. "Effects of Reproduction On Immuno-suppression and Oxidative Damage, and Hence Support or Otherwise for Their Roles as Mechanisms Underpinning Life History Trade-offs, Are Tissue and Assay Dependent." The Journal of Experimental Biology, vol. 216, no. Pt 22, 2013, pp. 4242-50.
Yang DB, Xu YC, Wang DH, et al. Effects of reproduction on immuno-suppression and oxidative damage, and hence support or otherwise for their roles as mechanisms underpinning life history trade-offs, are tissue and assay dependent. J Exp Biol. 2013;216(Pt 22):4242-50.
Yang, D. B., Xu, Y. C., Wang, D. H., & Speakman, J. R. (2013). Effects of reproduction on immuno-suppression and oxidative damage, and hence support or otherwise for their roles as mechanisms underpinning life history trade-offs, are tissue and assay dependent. The Journal of Experimental Biology, 216(Pt 22), 4242-50. https://doi.org/10.1242/jeb.092049
Yang DB, et al. Effects of Reproduction On Immuno-suppression and Oxidative Damage, and Hence Support or Otherwise for Their Roles as Mechanisms Underpinning Life History Trade-offs, Are Tissue and Assay Dependent. J Exp Biol. 2013 Nov 15;216(Pt 22):4242-50. PubMed PMID: 23997195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of reproduction on immuno-suppression and oxidative damage, and hence support or otherwise for their roles as mechanisms underpinning life history trade-offs, are tissue and assay dependent. AU - Yang,Deng-Bao, AU - Xu,Yan-Chao, AU - Wang,De-Hua, AU - Speakman,John R, Y1 - 2013/08/30/ PY - 2013/9/3/entrez PY - 2013/9/3/pubmed PY - 2014/6/7/medline KW - Meriones unguiculatus KW - Mongolian gerbil KW - environmental temperature KW - immune function KW - lactation KW - oxidative damage KW - trade-offs SP - 4242 EP - 50 JF - The Journal of experimental biology JO - J. Exp. Biol. VL - 216 IS - Pt 22 N2 - Life history parameters appear to be traded off against each other, but the physiological mechanisms involved remain unclear. One hypothesis is that potentially energetically costly processes such as immune function and protection from oxidative stress may be compromised during reproductive attempts because of selective resource allocation. Lower temperatures also impose energy costs, and hence allocation decisions might be more pronounced when animals are forced to reproduce in the cold. Here, we experimentally tested whether reproduction at different ambient temperatures was associated with elevated oxidative stress and suppressed immune function in Mongolian gerbils (Meriones unguiculatus). Using a variety of different markers for both immune function and oxidative stress, we found that some measures of immune function (serum bactericidal capacity and size of the thymus) were significantly suppressed, while some measures of oxidative protection [serum superoxide dismutase (SOD) activity and glutathione peroxidase (GPx) activity] were also reduced, and a marker of oxidative damage (protein carbonyls in serum) was increased in lactating compared with non-reproductive gerbils. These changes were in line with the selective resource allocation predictions. However, the phytohaemagglutinin response and serum total immunoglobulin (IgG) were not suppressed, and other markers of oxidative damage [malondialdehyde (MDA) (TBARS) and protein carbonyls in the liver] were actually lower in lactating compared with non-reproductive gerbils, consistent with increased levels of SOD activity and total antioxidant capacity in the liver. These latter changes were opposite of the expectations based on resource allocation. Furthermore, other measures of protection (GPx levels in the liver and protein thiols in both serum and liver) and damage [MDA (TBARS) in serum] were unrelated to reproductive status. Ambient temperature differences did not impact on these patterns. Collectively, our results indicated that the inferred effects of reproduction on immunosuppression and oxidative damage, and hence support or otherwise for particular physiological mechanisms that underpin life history trade-offs, are critically dependent on the exact markers and tissues used. This may be because during reproduction individuals selectively allocate protection to some key tissues, but sacrifice protection of others. SN - 1477-9145 UR - https://www.unboundmedicine.com/medline/citation/23997195/Effects_of_reproduction_on_immuno_suppression_and_oxidative_damage_and_hence_support_or_otherwise_for_their_roles_as_mechanisms_underpinning_life_history_trade_offs_are_tissue_and_assay_dependent_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=23997195 DB - PRIME DP - Unbound Medicine ER -