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Combined antibacterial activity of stingless bee (Apis mellipodae) honey and garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria.
Asian Pac J Trop Biomed. 2013 Sep; 3(9):725-31.AP

Abstract

OBJECTIVE

To investigate the synergic antibacterial activity of garlic and tazma honey against standard and clinical pathogenic bacteria.

METHODS

Antimicrobial activity of tazma honey, garlic and mixture of them against pathogenic bacteria were determined. Chloramphenicol and water were used as positive and negative controls, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration of antimicrobial samples were determined using standard methods.

RESULTS

Inhibition zone of mixture of garlic and tazma honey against all tested pathogens was significantly (P≤0.05) greater than garlic and tazma honey alone. The diameter zone of inhibition ranged from (18±1) to (35±1) mm for mixture of garlic and tazma honey, (12±1) to (20±1) mm for tazma honey and (14±1) to (22±1) mm for garlic as compared with (10±1) to (30±1) mm for chloramphenicol. The combination of garlic and tazma honey (30-35 mm) was more significantly (P≤0.05) effective against Salmonella (NCTC 8385), Staphylococcus aureus (ATCC 25923), Lyesria moncytogenes (ATCC 19116) and Streptococcus pneumonia (ATCC 63). Results also showed considerable antimicrobial activity of garlic and tazma honey. MIC of mixture of garlic and tazma honey at 6.25% against total test bacteria was 88.9%. MIC of mixture of garlic and tazma honey at 6.25% against Gram positive and negative were 100% and 83.33%, respectively. The bactericidal activities of garlic, tazma honey, and mixture of garlic and tazma honey against all pathogenic bacteria at 6.25% concentration were 66.6%, 55.6% and 55.6%, respectively.

CONCLUSIONS

This finding strongly supports the claim of the local community to use the combination of tazma honey and garlic for the treatment of different pathogenic bacterial infections. Therefore, garlic in combination with tazma honey can serve as an alternative natural antimicrobial drug for the treatment of pathogenic bacterial infections. Further in vivo study is recommended to come up with a comprehensive conclusion.

Authors+Show Affiliations

Department of Biotechnology, Natural and Computational Sciences Faculty, University of Gondar, Gondar, Ethiopia. anberhanu2007@yahoo.com

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23998014

Citation

Andualem, Berhanu. "Combined Antibacterial Activity of Stingless Bee (Apis Mellipodae) Honey and Garlic (Allium Sativum) Extracts Against Standard and Clinical Pathogenic Bacteria." Asian Pacific Journal of Tropical Biomedicine, vol. 3, no. 9, 2013, pp. 725-31.
Andualem B. Combined antibacterial activity of stingless bee (Apis mellipodae) honey and garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria. Asian Pac J Trop Biomed. 2013;3(9):725-31.
Andualem, B. (2013). Combined antibacterial activity of stingless bee (Apis mellipodae) honey and garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria. Asian Pacific Journal of Tropical Biomedicine, 3(9), 725-31. https://doi.org/10.1016/S2221-1691(13)60146-X
Andualem B. Combined Antibacterial Activity of Stingless Bee (Apis Mellipodae) Honey and Garlic (Allium Sativum) Extracts Against Standard and Clinical Pathogenic Bacteria. Asian Pac J Trop Biomed. 2013;3(9):725-31. PubMed PMID: 23998014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined antibacterial activity of stingless bee (Apis mellipodae) honey and garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria. A1 - Andualem,Berhanu, PY - 2013/05/25/received PY - 2013/07/20/accepted PY - 2013/9/3/entrez PY - 2013/9/3/pubmed PY - 2014/5/16/medline KW - Antimicrobial activity KW - Bactericidal activity KW - Garlic KW - Honey KW - Inhibition zone KW - Minimum bactericidal concentration KW - Minimum inhibitory concentration SP - 725 EP - 31 JF - Asian Pacific journal of tropical biomedicine JO - Asian Pac J Trop Biomed VL - 3 IS - 9 N2 - OBJECTIVE: To investigate the synergic antibacterial activity of garlic and tazma honey against standard and clinical pathogenic bacteria. METHODS: Antimicrobial activity of tazma honey, garlic and mixture of them against pathogenic bacteria were determined. Chloramphenicol and water were used as positive and negative controls, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration of antimicrobial samples were determined using standard methods. RESULTS: Inhibition zone of mixture of garlic and tazma honey against all tested pathogens was significantly (P≤0.05) greater than garlic and tazma honey alone. The diameter zone of inhibition ranged from (18±1) to (35±1) mm for mixture of garlic and tazma honey, (12±1) to (20±1) mm for tazma honey and (14±1) to (22±1) mm for garlic as compared with (10±1) to (30±1) mm for chloramphenicol. The combination of garlic and tazma honey (30-35 mm) was more significantly (P≤0.05) effective against Salmonella (NCTC 8385), Staphylococcus aureus (ATCC 25923), Lyesria moncytogenes (ATCC 19116) and Streptococcus pneumonia (ATCC 63). Results also showed considerable antimicrobial activity of garlic and tazma honey. MIC of mixture of garlic and tazma honey at 6.25% against total test bacteria was 88.9%. MIC of mixture of garlic and tazma honey at 6.25% against Gram positive and negative were 100% and 83.33%, respectively. The bactericidal activities of garlic, tazma honey, and mixture of garlic and tazma honey against all pathogenic bacteria at 6.25% concentration were 66.6%, 55.6% and 55.6%, respectively. CONCLUSIONS: This finding strongly supports the claim of the local community to use the combination of tazma honey and garlic for the treatment of different pathogenic bacterial infections. Therefore, garlic in combination with tazma honey can serve as an alternative natural antimicrobial drug for the treatment of pathogenic bacterial infections. Further in vivo study is recommended to come up with a comprehensive conclusion. SN - 2221-1691 UR - https://www.unboundmedicine.com/medline/citation/23998014/Combined_antibacterial_activity_of_stingless_bee__Apis_mellipodae__honey_and_garlic__Allium_sativum__extracts_against_standard_and_clinical_pathogenic_bacteria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/apjtb-03-09-725 DB - PRIME DP - Unbound Medicine ER -