Tags

Type your tag names separated by a space and hit enter

Graphene oxide based ultrasensitive flow-through chemiluminescent immunoassay for sub-picogram level detection of chicken interferon-γ.
Biosens Bioelectron. 2014 Jan 15; 51:356-61.BB

Abstract

The quantitative detection of chicken interferon-γ (ChIFN-γ) released by T cells after in vitro stimulation is a good evaluation of cell-mediated immunity in chickens after infection or vaccination. This work reports a new flow-through the chemiluminescent (CL) immunoassay method for rapid and specific determination of ChIFN-γ at a level of sub-picogram per milliliter. The biocompatible graphene oxide nanosheet is introduced into CL immunoassay for highly efficient immobilization of capture antibody. The detection limit of the proposed method at a signal to noise ratio of 3 is 0.36 pg ml(-1), which is 138-fold lower than the current lowest value of 50 pg ml(-1) for ChIFN-γ. Coupling with a flow-through system, the whole immunoassay process can be completed within 25 min. The resulting ChIFN-γ immunosensor shows excellent detection and fabrication reproducibility, good specificity and stability. The assay results of nature ChIFN-γ samples with the proposed method are in an acceptable agreement with the reference values. Compared to the present assay methods, this method is more flexible, simple, rapid and sensitive. The aim of this work is to demonstrate that the ultrasensitive, specific and rapid CL immunoassay format can become a very potential application for quantifying ChIFN-γ and further studying its role in immune response in poultry.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering; School of Medicine; Jiangsu Key Lab of Zoonosis, Yangzhou University, Yangzhou 225002, PR China. Electronic address: zjyang@yzu.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23999207

Citation

Yang, Zhanjun, et al. "Graphene Oxide Based Ultrasensitive Flow-through Chemiluminescent Immunoassay for Sub-picogram Level Detection of Chicken Interferon-γ." Biosensors & Bioelectronics, vol. 51, 2014, pp. 356-61.
Yang Z, Zhu J, Dai H, et al. Graphene oxide based ultrasensitive flow-through chemiluminescent immunoassay for sub-picogram level detection of chicken interferon-γ. Biosens Bioelectron. 2014;51:356-61.
Yang, Z., Zhu, J., Dai, H., Li, J., Shen, J., Jiao, X., Hu, X., & Ju, H. (2014). Graphene oxide based ultrasensitive flow-through chemiluminescent immunoassay for sub-picogram level detection of chicken interferon-γ. Biosensors & Bioelectronics, 51, 356-61. https://doi.org/10.1016/j.bios.2013.07.067
Yang Z, et al. Graphene Oxide Based Ultrasensitive Flow-through Chemiluminescent Immunoassay for Sub-picogram Level Detection of Chicken Interferon-γ. Biosens Bioelectron. 2014 Jan 15;51:356-61. PubMed PMID: 23999207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Graphene oxide based ultrasensitive flow-through chemiluminescent immunoassay for sub-picogram level detection of chicken interferon-γ. AU - Yang,Zhanjun, AU - Zhu,Jing, AU - Dai,Hua, AU - Li,Juan, AU - Shen,Juan, AU - Jiao,Xinan, AU - Hu,Xiaoya, AU - Ju,Huangxian, Y1 - 2013/08/12/ PY - 2013/05/18/received PY - 2013/07/09/revised PY - 2013/07/31/accepted PY - 2013/9/4/entrez PY - 2013/9/4/pubmed PY - 2014/5/13/medline KW - Chemiluminescent biosensing KW - Chicken interferon-γ KW - Cytokine KW - Flow-through analysis KW - Graphene oxide KW - Immunoassay SP - 356 EP - 61 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 51 N2 - The quantitative detection of chicken interferon-γ (ChIFN-γ) released by T cells after in vitro stimulation is a good evaluation of cell-mediated immunity in chickens after infection or vaccination. This work reports a new flow-through the chemiluminescent (CL) immunoassay method for rapid and specific determination of ChIFN-γ at a level of sub-picogram per milliliter. The biocompatible graphene oxide nanosheet is introduced into CL immunoassay for highly efficient immobilization of capture antibody. The detection limit of the proposed method at a signal to noise ratio of 3 is 0.36 pg ml(-1), which is 138-fold lower than the current lowest value of 50 pg ml(-1) for ChIFN-γ. Coupling with a flow-through system, the whole immunoassay process can be completed within 25 min. The resulting ChIFN-γ immunosensor shows excellent detection and fabrication reproducibility, good specificity and stability. The assay results of nature ChIFN-γ samples with the proposed method are in an acceptable agreement with the reference values. Compared to the present assay methods, this method is more flexible, simple, rapid and sensitive. The aim of this work is to demonstrate that the ultrasensitive, specific and rapid CL immunoassay format can become a very potential application for quantifying ChIFN-γ and further studying its role in immune response in poultry. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23999207/Graphene_oxide_based_ultrasensitive_flow_through_chemiluminescent_immunoassay_for_sub_picogram_level_detection_of_chicken_interferon_γ_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00540-X DB - PRIME DP - Unbound Medicine ER -