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Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine.
Acta Crystallogr D Biol Crystallogr 2013; 69(Pt 9):1850-60AC

Abstract

A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the `core' structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (λex/λem = 502/511 nm) and red laRFP (λex/λem ≃ 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-ΔS83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ∼20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C(β) atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (∼70 nm) of laRFP was verified by extensive structure-based site-directed mutagenesis.

Authors+Show Affiliations

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23999308

Citation

Pletnev, Vladimir Z., et al. "Structure of the Red Fluorescent Protein From a Lancelet (Branchiostoma Lanceolatum): a Novel GYG Chromophore Covalently Bound to a Nearby Tyrosine." Acta Crystallographica. Section D, Biological Crystallography, vol. 69, no. Pt 9, 2013, pp. 1850-60.
Pletnev VZ, Pletneva NV, Lukyanov KA, et al. Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine. Acta Crystallogr D Biol Crystallogr. 2013;69(Pt 9):1850-60.
Pletnev, V. Z., Pletneva, N. V., Lukyanov, K. A., Souslova, E. A., Fradkov, A. F., Chudakov, D. M., ... Pletnev, S. (2013). Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine. Acta Crystallographica. Section D, Biological Crystallography, 69(Pt 9), pp. 1850-60. doi:10.1107/S0907444913015424.
Pletnev VZ, et al. Structure of the Red Fluorescent Protein From a Lancelet (Branchiostoma Lanceolatum): a Novel GYG Chromophore Covalently Bound to a Nearby Tyrosine. Acta Crystallogr D Biol Crystallogr. 2013;69(Pt 9):1850-60. PubMed PMID: 23999308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine. AU - Pletnev,Vladimir Z, AU - Pletneva,Nadya V, AU - Lukyanov,Konstantin A, AU - Souslova,Ekaterina A, AU - Fradkov,Arkady F, AU - Chudakov,Dmitry M, AU - Chepurnykh,Tatyana, AU - Yampolsky,Ilia V, AU - Wlodawer,Alexander, AU - Dauter,Zbigniew, AU - Pletnev,Sergei, Y1 - 2013/08/17/ PY - 2013/05/08/received PY - 2013/06/03/accepted PY - 2013/9/4/entrez PY - 2013/9/4/pubmed PY - 2014/3/13/medline KW - Branchiostoma lanceolatum KW - GYG chromophore KW - lancelets KW - red fluorescent proteins SP - 1850 EP - 60 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr. D Biol. Crystallogr. VL - 69 IS - Pt 9 N2 - A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the `core' structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (λex/λem = 502/511 nm) and red laRFP (λex/λem ≃ 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-ΔS83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ∼20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C(β) atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (∼70 nm) of laRFP was verified by extensive structure-based site-directed mutagenesis. SN - 1399-0047 UR - https://www.unboundmedicine.com/medline/citation/23999308/Structure_of_the_red_fluorescent_protein_from_a_lancelet__Branchiostoma_lanceolatum_:_a_novel_GYG_chromophore_covalently_bound_to_a_nearby_tyrosine_ L2 - http://scripts.iucr.org/cgi-bin/paper?S0907444913015424 DB - PRIME DP - Unbound Medicine ER -