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Successful fertilization requires the presence of at least one major O-acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis.
Plant Physiol 2013; 163(2):959-72PP

Abstract

The synthesis of cysteine (Cys) is a master control switch of plant primary metabolism that coordinates the flux of sulfur with carbon and nitrogen metabolism. In Arabidopsis (Arabidopsis thaliana), nine genes encode for O-acetylserine(thiol)lyase (OAS-TL)-like proteins, of which the major isoforms, OAS-TL A, OAS-TL B, and OAS-TL C, catalyze the formation of Cys by combining O-acetylserine and sulfide in the cytosol, the plastids, and the mitochondria, respectively. So far, the significance of individual OAS-TL-like enzymes is unresolved. Generation of all major OAS-TL double loss-of-function mutants in combination with radiolabeled tracer studies revealed that subcellular localization of OAS-TL proteins is more important for efficient Cys synthesis than total cellular OAS-TL activity in leaves. The absence of oastl triple embryos after targeted crosses indicated the exclusiveness of Cys synthesis by the three major OAS-TLs and ruled out alternative sulfur fixation by other OAS-TL-like proteins. Analyses of oastlABC pollen demonstrated that the presence of at least one functional OAS-TL isoform is essential for the proper function of the male gametophyte, although the synthesis of histidine, lysine, and tryptophan is dispensable in pollen. Comparisons of oastlABC pollen derived from genetically different parent plant combinations allowed us to separate distinct functions of Cys and glutathione in pollen and revealed an additional role of glutathione for pollen germination. In contrast, female gametogenesis was not affected by the absence of major OAS-TLs, indicating significant transport of Cys into the developing ovule from the mother plant.

Authors+Show Affiliations

Centre for Organismal Studies Heidelberg, Department of Plant Molecular Biology , University of Heidelberg, 69120 Heidelberg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24001608

Citation

Birke, Hannah, et al. "Successful Fertilization Requires the Presence of at Least One Major O-acetylserine(thiol)lyase for Cysteine Synthesis in Pollen of Arabidopsis." Plant Physiology, vol. 163, no. 2, 2013, pp. 959-72.
Birke H, Heeg C, Wirtz M, et al. Successful fertilization requires the presence of at least one major O-acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis. Plant Physiol. 2013;163(2):959-72.
Birke, H., Heeg, C., Wirtz, M., & Hell, R. (2013). Successful fertilization requires the presence of at least one major O-acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis. Plant Physiology, 163(2), pp. 959-72. doi:10.1104/pp.113.221200.
Birke H, et al. Successful Fertilization Requires the Presence of at Least One Major O-acetylserine(thiol)lyase for Cysteine Synthesis in Pollen of Arabidopsis. Plant Physiol. 2013;163(2):959-72. PubMed PMID: 24001608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Successful fertilization requires the presence of at least one major O-acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis. AU - Birke,Hannah, AU - Heeg,Corinna, AU - Wirtz,Markus, AU - Hell,Rüdiger, Y1 - 2013/09/03/ PY - 2013/9/5/entrez PY - 2013/9/5/pubmed PY - 2014/5/7/medline SP - 959 EP - 72 JF - Plant physiology JO - Plant Physiol. VL - 163 IS - 2 N2 - The synthesis of cysteine (Cys) is a master control switch of plant primary metabolism that coordinates the flux of sulfur with carbon and nitrogen metabolism. In Arabidopsis (Arabidopsis thaliana), nine genes encode for O-acetylserine(thiol)lyase (OAS-TL)-like proteins, of which the major isoforms, OAS-TL A, OAS-TL B, and OAS-TL C, catalyze the formation of Cys by combining O-acetylserine and sulfide in the cytosol, the plastids, and the mitochondria, respectively. So far, the significance of individual OAS-TL-like enzymes is unresolved. Generation of all major OAS-TL double loss-of-function mutants in combination with radiolabeled tracer studies revealed that subcellular localization of OAS-TL proteins is more important for efficient Cys synthesis than total cellular OAS-TL activity in leaves. The absence of oastl triple embryos after targeted crosses indicated the exclusiveness of Cys synthesis by the three major OAS-TLs and ruled out alternative sulfur fixation by other OAS-TL-like proteins. Analyses of oastlABC pollen demonstrated that the presence of at least one functional OAS-TL isoform is essential for the proper function of the male gametophyte, although the synthesis of histidine, lysine, and tryptophan is dispensable in pollen. Comparisons of oastlABC pollen derived from genetically different parent plant combinations allowed us to separate distinct functions of Cys and glutathione in pollen and revealed an additional role of glutathione for pollen germination. In contrast, female gametogenesis was not affected by the absence of major OAS-TLs, indicating significant transport of Cys into the developing ovule from the mother plant. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/24001608/Successful_fertilization_requires_the_presence_of_at_least_one_major_O_acetylserine_thiol_lyase_for_cysteine_synthesis_in_pollen_of_Arabidopsis_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=24001608 DB - PRIME DP - Unbound Medicine ER -