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Biosafety assessment of titanium dioxide nanoparticles in acutely exposed nematode Caenorhabditis elegans with mutations of genes required for oxidative stress or stress response.
Chemosphere. 2013 Nov; 93(10):2289-96.C

Abstract

We used Caenorhabditis elegans to investigate whether acute exposure to TiO2-NPs at the concentration of 20 μg L(-1) reflecting predicted environmental relevant concentration and 25 mg L(-1) reflecting concentration in food can cause toxicity on nematodes with mutations of susceptible genes. Among examined mutants associated with oxidative stress and stress response, we found that genes of sod-2, sod-3, mtl-2, and hsp-16.48 might be susceptible for TiO2-NPs toxicity. Mutations of these genes altered functions of both possible primary and secondary targeted organs in nematodes exposed to 25 mg L(-1) of TiO2-NPs for 24-h. Mutations of these genes caused similar expression patterns of genes required for oxidative stress in TiO2-NPs exposed mutant nematodes, implying their similar mechanisms to form the susceptible property. Nevertheless, acute exposure to 20 μg L(-1) of TiO2-NPs for 24-h and 25 mg L(-1) of TiO2-NPs for 0.48-h or 5.71-h did not influence functions of both possible primary and secondary targeted organs in sod-2, sod-3, mtl-2, and hsp-16.48 mutants. Therefore, our results suggest the relatively safe property of acute exposure to TiO2-NPs with certain durations at predicted environmental relevant concentrations or concentrations comparable to those in food in nematodes with mutations of some susceptible genes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rui Q
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China.
Zhao Y
No affiliation info available
Wu Q
No affiliation info available
Tang M
No affiliation info available
Wang D
No affiliation info available

MeSH

AnimalsCaenorhabditis elegansMutationNanoparticlesOxidative StressRisk AssessmentTitaniumToxicity Tests, Acute

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24001673

Citation

Rui, Qi, et al. "Biosafety Assessment of Titanium Dioxide Nanoparticles in Acutely Exposed Nematode Caenorhabditis Elegans With Mutations of Genes Required for Oxidative Stress or Stress Response." Chemosphere, vol. 93, no. 10, 2013, pp. 2289-96.
Rui Q, Zhao Y, Wu Q, et al. Biosafety assessment of titanium dioxide nanoparticles in acutely exposed nematode Caenorhabditis elegans with mutations of genes required for oxidative stress or stress response. Chemosphere. 2013;93(10):2289-96.
Rui, Q., Zhao, Y., Wu, Q., Tang, M., & Wang, D. (2013). Biosafety assessment of titanium dioxide nanoparticles in acutely exposed nematode Caenorhabditis elegans with mutations of genes required for oxidative stress or stress response. Chemosphere, 93(10), 2289-96. https://doi.org/10.1016/j.chemosphere.2013.08.007
Rui Q, et al. Biosafety Assessment of Titanium Dioxide Nanoparticles in Acutely Exposed Nematode Caenorhabditis Elegans With Mutations of Genes Required for Oxidative Stress or Stress Response. Chemosphere. 2013;93(10):2289-96. PubMed PMID: 24001673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosafety assessment of titanium dioxide nanoparticles in acutely exposed nematode Caenorhabditis elegans with mutations of genes required for oxidative stress or stress response. AU - Rui,Qi, AU - Zhao,Yunli, AU - Wu,Qiuli, AU - Tang,Meng, AU - Wang,Dayong, Y1 - 2013/08/31/ PY - 2013/06/18/received PY - 2013/07/27/revised PY - 2013/08/02/accepted PY - 2013/9/5/entrez PY - 2013/9/5/pubmed PY - 2014/5/29/medline KW - Acute exposure KW - Caenorhabditis elegans KW - Susceptible gene KW - TiO(2)-NPs KW - Toxicity assessment SP - 2289 EP - 96 JF - Chemosphere JO - Chemosphere VL - 93 IS - 10 N2 - We used Caenorhabditis elegans to investigate whether acute exposure to TiO2-NPs at the concentration of 20 μg L(-1) reflecting predicted environmental relevant concentration and 25 mg L(-1) reflecting concentration in food can cause toxicity on nematodes with mutations of susceptible genes. Among examined mutants associated with oxidative stress and stress response, we found that genes of sod-2, sod-3, mtl-2, and hsp-16.48 might be susceptible for TiO2-NPs toxicity. Mutations of these genes altered functions of both possible primary and secondary targeted organs in nematodes exposed to 25 mg L(-1) of TiO2-NPs for 24-h. Mutations of these genes caused similar expression patterns of genes required for oxidative stress in TiO2-NPs exposed mutant nematodes, implying their similar mechanisms to form the susceptible property. Nevertheless, acute exposure to 20 μg L(-1) of TiO2-NPs for 24-h and 25 mg L(-1) of TiO2-NPs for 0.48-h or 5.71-h did not influence functions of both possible primary and secondary targeted organs in sod-2, sod-3, mtl-2, and hsp-16.48 mutants. Therefore, our results suggest the relatively safe property of acute exposure to TiO2-NPs with certain durations at predicted environmental relevant concentrations or concentrations comparable to those in food in nematodes with mutations of some susceptible genes. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/24001673/Biosafety_assessment_of_titanium_dioxide_nanoparticles_in_acutely_exposed_nematode_Caenorhabditis_elegans_with_mutations_of_genes_required_for_oxidative_stress_or_stress_response_ DB - PRIME DP - Unbound Medicine ER -