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[Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A].
Vopr Virusol. 2013 May-Jun; 58(3):21-5.VV

Abstract

Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested. A covalent linkage of flagellin with M2e significant increased the immunogenicity of the target antigen compared with non-covalent interaction M2e and HBc. Flagellin as a protein carrier of M2e induced mainly IgG1 subclass, whereas HBc stimulated more balanced Th1/Th2 response. Our study showed a decrease in the viral titers in lung tissues of immunized mice after lethal challenge of A/PR/8/34 (H1N1). The study revealed a possibility to obtain a vaccine preparation with equal immunogenicity both against human influenza viruses and highly pathogenic avian influenza viruses.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

rus

PubMed ID

24006628

Citation

Stepanova, L A., et al. "[Immunogenicity of Recombinant Proteins Including Ectodomain of M2 Influenza Virus A]." Voprosy Virusologii, vol. 58, no. 3, 2013, pp. 21-5.
Stepanova LA, Kovaleva AA, Potapchuk MV, et al. [Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A]. Vopr Virusol. 2013;58(3):21-5.
Stepanova, L. A., Kovaleva, A. A., Potapchuk, M. V., Korotkov, A. V., Kupriianov, V. V., Blokhina, E. A., Kotliarov, R. I. u., & Tsybalova, L. M. (2013). [Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A]. Voprosy Virusologii, 58(3), 21-5.
Stepanova LA, et al. [Immunogenicity of Recombinant Proteins Including Ectodomain of M2 Influenza Virus A]. Vopr Virusol. 2013 May-Jun;58(3):21-5. PubMed PMID: 24006628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Immunogenicity of recombinant proteins including ectodomain of M2 influenza virus A]. AU - Stepanova,L A, AU - Kovaleva,A A, AU - Potapchuk,M V, AU - Korotkov,A V, AU - Kupriianov,V V, AU - Blokhina,E A, AU - Kotliarov,R Iu, AU - Tsybalova,L M, PY - 2013/9/7/entrez PY - 2013/9/7/pubmed PY - 2013/9/27/medline SP - 21 EP - 5 JF - Voprosy virusologii JO - Vopr Virusol VL - 58 IS - 3 N2 - Two recombinant proteins with three copies of the ectodomain of the conserved influenza protein M2 (M2e) of influenza viruses were developed: A (H1N1)pdm09, A/Kurgan/05/05 (H5N1), and M2e consensus sequence of the human influenza A virus (H1N1, H2N2, H3N2) based on flagellin and core antigen of hepatitis B (HBc). The first recombinant protein comprised flagellin fused to three tandem copies of M2e, the second preparation was based on non-covalent interaction between M2e peptides and HBc. The immunogenicity of two preparations was comparatively tested. A covalent linkage of flagellin with M2e significant increased the immunogenicity of the target antigen compared with non-covalent interaction M2e and HBc. Flagellin as a protein carrier of M2e induced mainly IgG1 subclass, whereas HBc stimulated more balanced Th1/Th2 response. Our study showed a decrease in the viral titers in lung tissues of immunized mice after lethal challenge of A/PR/8/34 (H1N1). The study revealed a possibility to obtain a vaccine preparation with equal immunogenicity both against human influenza viruses and highly pathogenic avian influenza viruses. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/24006628/[Immunogenicity_of_recombinant_proteins_including_ectodomain_of_M2_influenza_virus_A]_ L2 - https://medlineplus.gov/flu.html DB - PRIME DP - Unbound Medicine ER -